Screening for S32G mutation of BMP15 gene in 18 goat breeds

Feng, Tao; Chu, Mingxing; Cao, G. L.; Huang, Dongwei; Di, Ran

doi:10.3906/vet-1401-97

Cited by 5 publications

(6 citation statements)

References 16 publications

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“…They suggested that BMP15 gene may be a major gene which affects the prolificacy in Funiu white goats. Feng et al (40) identified one mutation (A898 G) in BMP15 gene in Chinese goats and the allele G was found to be associated with high litter size in Jining Grey goats and Matou goats. This mutation is a nonsynonymous mutation which changes serine to glycine in the putative protein.…”

Section: Resultsmentioning

confidence: 98%

Genotyping of Novel SNPs in BMPR1B, BMP15, and GDF9 Genes for Association with Prolificacy in Seven Indian Goat Breeds

Ahlawat

Sharma

Roy

et al. 2016

Animal Biotechnology

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Goats form the backbone of rural livelihood and financial security systems in India but their population is showing decreasing trend. Improvement of reproductive traits such as prolificacy offers a solution to stabilize the decreasing goat population and to meet the nutritional needs of growing human population. In the present study, six novel SNPs in three candidate genes for prolificacy (BMPR1B, BMP15, and GDF9) were genotyped in seven breeds of Indian goats to evaluate their association with litter size. Tetra primer ARMS-PCR and PCR-RFLP based protocols were developed for genotyping six novel SNPs, namely, T(-242)C in BMPR1B; G735A and C808G in BMP15; and C818T, A959C, and G1189A in GDF9 gene. The effect of breed was highly significant (p ≤ 0.01) on litter size but the effect of genotype was nonsignificant. The effect of parity on litter size was also significant in the prolific Black Bengal breed. The litter size differences observed between breeds are attributed to breed differences. Novel mutations observed at different loci in GDF9, BMP15, and BMPR1B genes do not contribute to the reproductive capability of the investigated breeds. Further studies with more number of breeds and animals exploring association of these novel SNPs with reproductive traits may be fruitful.

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Section: Resultsmentioning

confidence: 98%

Genotyping of Novel SNPs in BMPR1B, BMP15, and GDF9 Genes for Association with Prolificacy in Seven Indian Goat Breeds

Ahlawat

Sharma

Roy

et al. 2016

Animal Biotechnology

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“…To define the source of heterogeneity, we performed a sensitivity analysis by removing studies one by one. The results indicated that studies performed by Dong and Du (2010) on the Lubei White breed and by Feng et al (2014) and Moghadaszadeh et al (2015) influenced overall results of the meta-analysis by increasing the P value under an additive genetic model. One possible reason for this could be that studies with a larger Z value have SMD farther from overall SMD, which can lead to the increased P value.…”

Section: Discussionmentioning

confidence: 89%

“…On the contrary, sensitivity analysis showed significant difference in litter size by dropping studies performed by Dong and Du (2010) on the Lubei White breed and by Feng et al (2014) andMoghadaszadeh et al (2015) under the additive model (Table 8 and Fig. 3).…”

Section: Sensitivity Analysis and Publication Biasmentioning

confidence: 87%

“…For the variant c.963A>G in exon 2 of the BMP15 gene, ambiguous effects have been reported. Some researchers observed a significant effect of c.963A>G on litter size in goats (Chu et al, 2007;Feng et al, 2009;Dong and Du, 2010;Feng et al, 2014;Moghadaszadeh et al, 2015), whereas others did not find any association between this single-nucleotide polymorphism (SNP) and litter size (Dong and Du, 2010). Because of small sample sizes, some of the mentioned studies reported low statistical power to validate negative or positive effects of c.963A>G variants on litter size.…”

Section: Introductionmentioning

confidence: 99%

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Meta-analysis of association between c.963A>G single-nucleotide polymorphism on BMP15 gene and litter size in goats

et al. 2022

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Abstract. Litter size is an important economic trait in the goat industry. Previous studies on the bone morphogenetic protein 15 (BMP15) gene detected some single-nucleotide polymorphisms (SNPs) such as c.963A>G that were associated with an increase in ovulation rate and litter size. The aim of this study was to conduct a meta-analysis on the effect of this polymorphism on litter size. We gathered and pooled data from five eligible published studies. To investigate the effect of c.963A>G on litter size, we utilized four different genetic models assuming dominant (GG + GA vs. AA), recessive (GG vs. GA + AA), additive (GG vs. AA) and co-dominant (GG + AA vs. GA) model of inheritance. Data were analyzed under random-effects models based on the I2 value. Furthermore, sensitivity analysis was carried out to validate the stability of results. The results showed that the c.963A>G polymorphism is associated with litter size when applying a dominant model (standardized mean difference (SMD) is 0.815, 95 % CI [0.170, 1.461], P value = 0.013) and also with an additive model (SMD = 0.755, 95 % CI [0.111, 1.400], P value = 0.022). However, the effect of c.963A>G polymorphism was not significant under recessive (SMD = 0.186, 95 % CI [−0.195, 4.259], P value = 0.339) and co-dominant (SMD = −0.119, 95 % CI [−0.525, 0.288], P value = 0.568) models. Sensitivity analysis demonstrated that dropping studies with wide confidence intervals affects overall results under the assumption of an additive model. The meta-analysis results revealed that the AA genotype could be positively connected with litter size in goats.

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“…Theses base changes resulting in substitutions of amino acids that might be a major mechanism that caused the differential prolificacy of mentioned goat breeds. In Chinese goats, Feng et al (2014) detected by using PCR-RFLP one mutation, A898G in BMP15 gene. The results indicated that the allele G associated with high litter size in two strains of such goats, Jining Grey and Matou goats.…”

Section: Pcr-sscp Of Fecx I Of Bmp15 Genementioning

confidence: 99%

Effect of genetic polymorphism of BMP15 gene on improving twin production in Egyptian small ruminants

2018

Res. J. Ani. & Vet. Sci.

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The present work was designed to study the genetic polymorphism in BMP15 gene at four sites, FecX B , FecX G , FecX H and FecX I and its association with improving twin production of Egyptian sheep and goats. This study was performed on 139 females including 113 mothers (83 of sheep and 30 of goats) at first , second, third and fourth parity and 26 animals (12 of sheep and 14 of goats) at age of sexual maturity to quantify the females that have favorable gene marker for their utilizing in successful breeding program. Sheep breeds involved Barki, Osseimi, Rahmani, Saudanez and Awase, whereas, goats breeds involved Zaraibi, Damascus, Boer, Sanan and Barki. Blood samples were collected from above all animals and genomic DNA was extracted. The fragments at size of 153 bp, 141 bp, 240 bp and 154 were amplified in PCR conditions for FecX B , FecX G

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Screening for S32G mutation of BMP15 gene in 18 goat breeds

Abstract: doi:10.3906/vet-1401-9

Cited by 5 publications

References 16 publications

Genotyping of Novel SNPs in BMPR1B, BMP15, and GDF9 Genes for Association with Prolificacy in Seven Indian Goat Breeds

Genotyping of Novel SNPs in BMPR1B, BMP15, and GDF9 Genes for Association with Prolificacy in Seven Indian Goat Breeds

Meta-analysis of association between c.963A>G single-nucleotide polymorphism on BMP15 gene and litter size in goats

Effect of genetic polymorphism of BMP15 gene on improving twin production in Egyptian small ruminants

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