Association Between Microbial Bioburden and Healing Outcomes in Venous Leg Ulcers: A Review of the Evidence

Tuttle, Marie S.

doi:10.1089/wound.2014.0535

Cited by 43 publications

(43 citation statements)

References 48 publications

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“…While the value of NGS and advanced bioinformatics for the characterization of chronic wounds is widely accepted, their impact on individual therapeutic management decisions which might justify their use in spite of considerable costs is still poorly assessed (Tuttle, 2015). …”

Section: Common Sites Of Multiple Pathogen Detections and Interpretatmentioning

confidence: 99%

More Pathogenicity or Just More Pathogens?—On the Interpretation Problem of Multiple Pathogen Detections with Diagnostic Multiplex Assays

et al. 2017

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Modern molecular diagnostic approaches in the diagnostic microbiological laboratory like real-time quantitative polymerase chain reaction (qPCR) have led to a considerable increase of diagnostic sensitivity. They usually outperform the diagnostic sensitivity of culture-based approaches. Culture-based diagnostics were found to be insufficiently sensitive for the assessment of the composition of biofilms in chronic wounds and poorly standardized for screenings for enteric colonization with multi-drug resistant bacteria. However, the increased sensitivity of qPCR causes interpretative challenges regarding the attribution of etiological relevance to individual pathogen species in case of multiple detections of facultative pathogenic microorganisms in primarily non-sterile sample materials. This is particularly the case in high-endemicity settings, where continuous exposition to respective microorganisms leads to immunological adaptation and semi-resistance while considerable disease would result in case of exposition of a non-adapted population. While biofilms in chronic wounds show higher pathogenic potential in case of multi-species composition, detection of multiple pathogens in respiratory samples is much more difficult to interpret and asymptomatic enteric colonization with facultative pathogenic microorganisms is frequently observed in high endemicity settings. For respiratory samples and stool samples, cycle-threshold-value-based semi-quantitative interpretation of qPCR results has been suggested. Etiological relevance is assumed if cycle-threshold values are low, suggesting high pathogen loads. Although the procedure is challenged by lacking standardization and methodical issues, first evaluations have led to promising results. Future studies should aim at generally acceptable quantitative cut-off values to allow discrimination of asymptomatic colonization from clinically relevant infection.

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Section: Common Sites Of Multiple Pathogen Detections and Interpretatmentioning

confidence: 99%

More Pathogenicity or Just More Pathogens?—On the Interpretation Problem of Multiple Pathogen Detections with Diagnostic Multiplex Assays

et al. 2017

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“…3 Lack of adequate biofilm control and bacterial load implies in serious complications, such as delayed healing, cellulitis, and sepsis. 25 Removing biofilm is a major challenge for the health care provider because of its firm adherence to the surrounding tissue. Attinger and Wolcott 3 also emphasize that debridement of this hard-to-remove biofilm opens a time-dependent window (2-3 days in wounds) where topic agents might be more effective.…”

Section: Assessment Of the Patient With Vlumentioning

confidence: 99%

All about compression: A literature review

Carvalho

Andrade

Abreu

et al. 2016

Journal of Vascular Nursing

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“…Few antibiotics are available in the market, but the development of resistance by pathogens against particular antibiotic is a common process, thus a high dose of such antibiotic is required [37][38][39][40][41]. Wound healing is a very prevalent phenomenon, but in diabetic wound, the healing process gets delayed due to lowering of several physiological processes [42,43], which ultimately leads to infectious wounds and then delayed healing [44][45][46][47]. Hence, in this study, an ecofriendly approach for biosynthesizing metal nanoparticles has been applied, which is low cost, facilely scaled up, feasible, and may act as better therapeutic agent for wound management.…”

Section: Introductionmentioning

confidence: 99%

Effect of Silver Nanoparticle-Mediated Wound Therapy on Biochemical, Hematological, and Histological Parameters

Chauhan

Shrivastava

Prasad

et al. 2018

Asian J Pharm Clin Res

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Methods: Extraction of Syzygium cumini was done and was used for silver nanoparticle synthesis. The synthesized nanoparticles were characterized by ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy, scanning electron microscopy, and transmission electron microscopy, which confirms the availability of nanosilver particles with marginally spherical morphology. The particles were then carried forward for treating impaired diabetic wounds in Wistar rat models. Regular photography was done and wound healing parameters were monitored throughout the study along with analyzing other parameters such as biochemical, hematological, and histological parameters. Results:The study showed that the sizes of the synthesized nanoparticles are below 100 nm. The results obtained from in vivo studies showed efficient wound healing potential of silver nanoparticles as compared to pre-existing drug povidone-iodine, i.e., the percentage reduction in wound area after therapy is 96.09% in case of biosynthesized silver nanoparticle-treated group, 97.7% reduction in chemically synthesized silver nanoparticle-treated group, 64.28% reduction in case of pre-existing drug povidone-iodine-treated group, 37.5% reduction in case of diabetic control group (diabetic), and 97.5% reduction in normal control group (non-diabetic). Results showed that biosynthesized silver nanoparticles showed less toxicity with respect to liver and kidney functions. Skin histology results showed increased sign of wound healing in biosynthesized silver nanoparticles. Hematology results showed no such variation. Conclusion:The study will help to synthesize new economically viable potential biosynthesized nanoparticles along with providing the approach to develop the medication at nanoscale level.

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Association Between Microbial Bioburden and Healing Outcomes in Venous Leg Ulcers: A Review of the Evidence

Cited by 43 publications

References 48 publications

More Pathogenicity or Just More Pathogens?—On the Interpretation Problem of Multiple Pathogen Detections with Diagnostic Multiplex Assays

More Pathogenicity or Just More Pathogens?—On the Interpretation Problem of Multiple Pathogen Detections with Diagnostic Multiplex Assays

All about compression: A literature review

Effect of Silver Nanoparticle-Mediated Wound Therapy on Biochemical, Hematological, and Histological Parameters

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