Association between metabolic profile and microbiomic changes in rats with functional dyspepsia

Abstract

Functional dyspepsia (FD) is one of the most prevalent functional gastrointestinal disorders (FGIDs).Accumulated evidence has shown that FD is a metabolic disease that might relate to gut microbiota, but the relationship between microbiome and the host metabolic changes is still uncertain. To clarify the host-microbiota co-metabolism disorders related to FD, an integrated approach combining 1 H NMRbased metabolomics profiles, polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and 16S rRNA gene sequencing was used to investigate the relationship among FD, metabolism of gut microbiota and the host. 34 differential urinary metabolites and 19 differential fecal metabolites, which affected the metabolism of energy, amino acids, nucleotides and short chain fatty acids (SCFAs), were found to have associated with FD. Based on the receiver operating characteristic (ROC) analysis, 10 biomarkers were screened out as diagnostic markers of FD. Meanwhile, the concentrations of Flintibacter, Parasutterella, Eubacterium and Bacteroides significantly increased in the FD group, whereas Eisenbergiella, Butyrivibrio, Intestinimonas, Saccharofermentans, Acetivibrio, Lachnoanaerobaculum and Herbinix significantly decreased. Furthermore, the above altered microbiota revealed a strong correlation with the intermediate products of the tricarboxylic acid (TCA) cycle, amino acids and SCFAs. In our study, it suggested that the energy metabolism was mainly disturbed in FD rats. Our findings also demonstrated that FD might be the result of gut microbiota and metabolism disorders, which was potentially valuable to enrich our understanding of the pathogenesis of FD.

Understanding the mechanisms of efficacy of fecal microbiota transplant in treating recurrent Clostridioides difficile infection and beyond: the contribution of gut microbial-derived metabolites

“…The decrease in malonate after FMT has also not been described before. The capacity to metabolize malonate by the gut microbiota and its impact on host metabolism has not previously been characterized in detail, but appears to be negatively-correlated with acetate across different experimental landscapes, 44 , 45 and could be associated with an increase in gut bacteria using this metabolite as energy source.

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Therapeutic Assessment of Fractions of Hawthorn on Gastrointestinal Motility Disorder by UPLC-MS/MS-based Metabolomics

“…In this study, citric acid content in the gastrointestinal dysmotility model rats (induced by atropine sulfate) decreased when compared with the NC group, indicating that the TCA cycle metabolic pathway weakened [29][30][31]. After treatment of different polar parts of the products of hawthorn in the model rats, citric acid content and TCA cycle metabolism increased.…”

Urinary Metabolomic Profiling after Administration of Corydalis Tuber and Pharbitis Seed Extract in Healthy Korean Volunteers

“…Except for ε-(γ-glutamyl)-lysine, most of the candidates were found to affect the purine metabolic pathway, lipid, fatty acid metabolism, and lipid peroxidation. Most of the metabolite and microbiome changes in FD, previously analyzed in rats, belonged to the metabolism of energy, amino acids, nucleotides, and short chain fatty acids (SCFAs) [ 17 ]. Another study concluded that uric acid and metabolites involved in energy metabolism were somehow involved in dyspepsia, after administration of the Chinese medicine Weikangning for functional dyspepsia model rat [ 18 ].…”