Association between HLA-DQB1 and cervical dysplasia in Vietnamese women

Abstract

Host genetic background seems to play a key role in cervical carcinogenesis as only a small subset of women infected with high-risk human papillomaviruses (HPVs) develop cervical cancer. The rate of cervical cancer in Vietnamese women is notably high. To explore the association of human leukocyte antigen (HLA)-DQB1 alleles, HPV infection, and cervical dysplasia in this population, cervical smears were obtained from 101 women attending the obstetrics and gynecology clinic of Da Nang General Hospital in Vietnam. Besides the Papanicolaou test, HPV and HLA-DQB1 genotyping were performed using cervical smear DNA. Additionally, a subset of 30 blood samples was used as the gold standard for HLA genotyping. HLA-DQB1 alleles showed no association with HPV infection status. However, a positive association with cervical dysplasia was found for HLA-DQB1*0302 (P= 0.0229, relative risk (RR) = 4.737) and HLA-DQB1*0601 (P= 0.0370, RR = 4.038), whereas HLA-DQB1*0301 (P= 0.0152, RR = 0.221) was found negatively associated. The low diversity of HLA-DQB1 alleles, wide spectrum of HPV genotypes, and high prevalence of HPV 16 and HPV 18 observed in the study population suggest a permissive/susceptible genetic background that deserves further research. Total concordance of HLA-DQB1 genotyping results between blood and cervical cells confirms the potential value of cervical smears as an effective tool for the development of cervical cancer biomarkers.

Association between human leukocyte antigen class II alleles and human papillomavirus-mediated cervical cancer in Indian women

“…Individually, DQB1*0602 was not associated with cervical cancer cases; however, contrary reports are available from Tanzanian [41] populations and Honduran [42] and Swedish cervical cancer cases [27] with HR-HPV infection, as well as Iranian [43], Japanese [44], African American [40], and Vietnamese populations [45]. In addition, DQB1*06 in haplotypic association with TNF-11 (DQB1*06-TNF-11 haplotype) was also significantly associated with cervical cancer cases in a Swedish population [46].…”

A comprehensive review on host genetic susceptibility to human papillomavirus infection and progression to cervical cancer

“…[10111420–47] A protective association has also been found with DQA1*0501 and DQB1*0201, *0103, *0301/*0501, *04, *05, *050201, *06 alleles. [273334444548–51] DRB1 *4, *5, *11, *13, *15, *16 were found to be susceptibleloci. [12141530313638–40424351–57] and DR *1, *2, *4, *6, *12, *13, *14, *15 were found to be protective loci.…”

Optimized pH Method for DNA Elution from Buccal Cells Collected in Whatman FTA®Cards

“…To evaluate the applicability of the described procedure, DNA isolated from buccal cells and blood collected from two subjects were subjected to HLA-DQB1 genotyping using the Olerup SSP™ Kits (Olerup et al 1993;Olerup and Zetterquist 1992), which utilizes 60 ng of DNA per PCR amplification as described previously (Dao et al 2005;Lema et al 2006).…”