Association between HL‐A and Red Cell Antigens. An AutoAnalyzer Study

Abstract

Abstract. Sera containing cytotoxic HL‐A antibodies were examined in order to detect red cell antibodies, using an AutoAnalyzer. In sera with cytotoxic anti‐HL‐A2 antibodies, we were able to demonstrate haemagglutinins that reacted mainly with red cells from HL‐A28 positive donors. In sera with cytotoxic anti‐HL‐A8 antibodies, we found antibodies that reacted with red cells from some, although not all, HL‐A8 positive donors. The haemagglutinins gave indentical and highly reproducible reaction patterns, but there were marked variations in antigenic strength.

Cross-Reactions in the HLA System Revealed by Red Blood Cells Expressing HLA Determinants, with Particular Reference to Cross-Reaction between HLA-A2 and B17

“…By current lymphocyte cytotox icity techniques, no anti-HLA-B17 activity was demon strated in any of the sera. The two sera used in the absorption experiments, sera 90 and 120, have both been described in detail before [10]. The three anti-HLA-B17 sera (249, 424 and 443) have been tested in a large series on the AA with RBC from HLA-typed donors, and never found to react with the cells of any HLA-B17 negative donor.…”

“…HLA-A28 and A2 determinants in the HLA-A series, and HLA-B17 in the B series are fairly often expressed on RBC, as dem onstrated by sensitive techniques such as the AutoAnalyzer (AA) [10,13,14] and by cap illary methods [3][4][5]. HLA-B17 and HLA-A28 were previously known as the Table I.…”

“…In one of our previous studies, both sera were shown to contain anti-HLA-B7 related haemagglutinins [13]. Serum 158 was described in a previous study [10] . It showed strong anti-HLA-A2 and weak anti-HLA-B7 activity by cytotoxicity testing on lympho cytes, and haemagglutinating activity towards the same specificities as well as HLA-A28.…”

“…Parallel testing of haemagglutinating anti-HLA-A2/28 and anti-HLA-B17 sera on the AA with RBC from 29 HLA-B17 positive donors The HLA antisera were oligo/monospecific sera from women immunized in pregnancy, the HLA types of the serum donors or the immunizing cells are not known to us. The sera have been selected by cytotox icity testing with lymphocytes, and most of the anti-HLA-A2 sera have previously been tested for HLA haemagglutinating activity in large series [10,14], They had all shown haemagglutinating activity towards HLA-A2 and A28, but had not previously reacted with the RBC from any donor being HLA-A2 or A28 nega tive. However, as it appeared retrospectively, the cell panel did not contain RBC from any donor expressing HLA-B17 on the RBC.…”

“…Serum 534 is a fairly good oligospecific anti-HLA-B12 serum by lymphocyte cytotoxicity testing. The AA procedure for testing of HLA-related haemagglutinins has been described be fore [10]. Absorption experiments were always per formed with WBC, since RBC have been shown to absorb very little of the HLA-related haemagglutinins [11] .…”