Association Between Genetic Polymorphisms in the Promoter Regions of Let-7 and Risk of Papillary Thyroid Carcinoma

Abstract

The aim of this study was to investigate the association between 2 polymorphisms (ie, rs10877887 and rs13293512) in the promoter regions of let-7 and the risk of papillary thyroid carcinoma (PTC).A case-control study of 618 PTC patients and 562 controls was conducted. The rs10877887 polymorphism was genotyped by using polymerase chain reaction-restriction fragment length polymorphism and the rs13293512 polymorphism was genotyped by using a TaqMan Genotyping Assay. The results were confirmed by DNA sequencing.The rs10877887 polymorphism had reduced risks of PTC in heterozygous comparison, dominant model, and overdominant model (TC vs TT: adjusted odds ratio [OR] = 0.73, 95% confidence interval [95% CI] = 0.58–0.94, P = 0.01; TC/CC vs TT: adjusted OR = 0.79, 95% CI = 0.63–1.00, P = 0.047; TC vs TT/CC: adjusted OR = 0.73, 95% CI = 0.57–0.92, P = 0.007, respectively). Stratified analyses showed that PTC patients carrying the rs10877887 CC genotype were more likely to have multiple tumors (adjusted OR = 1.71, 95% CI = 1.03–2.86, P = 0.04), and PTC patients carrying the rs13293512 TC + CC or CC were more likely to develop N0 status (TC/CC vs TT: adjusted OR = 0.64, 95% CI = 0.43–0.94, P = 0.02; CC vs TC/TT: adjusted OR = 0.50, 95% CI = 0.33–0.77, P = 0.001, respectively).Our study suggests that the rs10877887 polymorphism may be associated with the risk of PTC and the rs13293512 polymorphism may correlate to lymph node metastasis in PTC.

Genetic polymorphisms of non-coding RNAs associated with increased head and neck cancer susceptibility: a systematic review and meta-analysis

“…Among them, two SNPs were located into lncRNAs, and the rest were located into miRNAs. The control group of nine cohorts from six studies [ 33 , 43 , 44 , 59 , 65 , 66 ] were not in agreement with HWE ( P > 0.05). Except all the three cohorts of miR-219-1 rs213210 and two of the four cohorts of miR-26a-1: rs7372209, the rest cohorts were of different SNPs (listed in detail in Supplementary Table 1 ).…”

“…The ethnic background of the participants included Asian [ 30 , 32 , 33 , 35 – 38 , 42 , 44 – 51 , 53 – 59 , 61 – 69 ], Black [ 43 ], Caucasian [ 28 , 31 , 34 , 39 – 41 , 52 , 60 ], and mixed [ 29 , 43 ]. Several cancer types were investigated, such as oesophageal cancer [ 30 , 32 , 42 – 44 , 46 , 47 , 49 , 53 , 55 , 57 , 58 , 60 , 63 , 65 , 68 ], head and neck squamous cell carcinoma (HNSCC) [ 29 , 31 , 35 , 40 ], nasopharyngeal carcinoma (NPC) [ 36 , 38 , 48 , 50 , 64 ], oral squamous cell carcinoma (OSCC) [ 33 , 37 , 41 , 51 , 52 , 54 , 59 , 62 ], and thyroid carcinoma [ 28 , 34 , 39 , 45 , 56 , 61 , 66 , 67 , 69 ]. It is important to mention that four studies [ 29 , 41 , 46 , ...…”

Serum miRNAs as Biomarkers for the Diagnosis and Prognosis of Thyroid Cancer: A Comprehensive Review of the Literature

“…The Rs712 sequence is located on the KRAS gene 3′-UTR mutant, which is targeted by Let-7. It has been confirmed that polymorphisms in this region is associated with the risk of developing thyroid cancer [40, 41]. Rs10877887 polymorphism, which is located in the promoter region of Let-7, may be at risk of developing thyroid cancer, and rs13293512 polymorphism, which is located in the promoter region of Let-7, may be linked with lymph node metastasis in thyroid cancer [41].…”

“…It has been confirmed that polymorphisms in this region is associated with the risk of developing thyroid cancer [40, 41]. Rs10877887 polymorphism, which is located in the promoter region of Let-7, may be at risk of developing thyroid cancer, and rs13293512 polymorphism, which is located in the promoter region of Let-7, may be linked with lymph node metastasis in thyroid cancer [41]. In a study, Let-7 expression was measured in the serum of 106 patients with thyroid cancer and was compared with that of 95 patients with benign glands and 45 patients without thyroid problems.…”

An rs4705342 T>C polymorphism in the promoter of miR-143/145 is associated with a decreased risk of ischemic stroke

“…Previous work has shown that single nucleotide polymorphisms (SNPs) in the promoters of miRNAs may modulate individual’s susceptibility to a variety of human diseases 20 21 22 23 24 25 26 27 28 29 30 . Recently, several SNPs in the promoter of miR-143/145 cluster have been identified 21 .…”