Association between expression of HOTAIR and invasiveness of gliomas, and its predictive value

Abstract

Association between expression of HOTAIR and invasiveness of gliomas, and its predictive value. AbstractBackground. Hox transcript antisense intergenic RNA (HOTAIR) is upregulated and associated with a poor prognosis in many cancer types. Besides, it is involved in the invasion and metastasis of non-small-cell lung cancer and nasopharyngeal carcinoma.Objectives. The aim of this study was to investigate the association between the expression of HOTAIR and the grades of gliomas, and to explore its possible mechanism, as well as to evaluate the value of HOTAIR applied in predicting the grades of gliomas. Material and methods.A total of 123 patients undergoing glioma surgeries were enrolled. Patients with grade I and grade II-IV tumors were regarded as the control group (n = 36) and the case group (n = 87), respectively. The expression of HOTAIR, matrix metalloproteinase 7 (MMP-7), matrix metalloproteinase 9 (MMP-9), and vascular endothelial growth factor (VEGF) was detected with quantitative reverse transcriptionpolymerase chain reaction (qRT-PCR) in glioma tissues and then compared between grade I and grades II-IV. The correlation between the relative expression of HOTAIR and that of MMP-7, MMP-9 and VEGF was analyzed. Multivariate analysis was performed to identify independent risk factors. Receiver operating characteristic (ROC) curve was employed to evaluate the predictive value.Results. The relative expression of HOTAIR, MMP-7, MMP-9, and VEGF was lower in glioma tissues of grade I than in the case of grades II-IV, and the relative expression of HOTAIR was positively correlated with the relative expression of MMP-7, MMP-9 and VEGF. Multivariate analysis showed that the relative expression of HOTAIR was independently associated with the grades of gliomas, but the relative expression of MMP-7, MMP-9 and VEGF was not. Besides, multivariate analysis showed that the expression level of HOTAIR >0.40 was an independent risk factor for grades II-IV after classifying the relative expression of HOTAIR, and ROC analysis showed that the expression level of HOTAIR >0.40 had a moderate value when applied in predicting grades II-IV. Conclusions.Hox transcript antisense intergenic RNA might promote the invasion of gliomas through upregulating the expression of MMP-7, MMP-9 and VEGF, and the expression level of HOTAIR >0.40 had a moderate value when applied in predicting grades II-IV.

Long Non-Coding RNA GABPB1-AS1 Augments Malignancy of Glioma Cells by Sequestering MicroRNA-330 and Reinforcing the ZNF367/Cell Cycle Signaling Pathway

“…26 Likewise, a hox transcript antisense intergenic RNA (HOTAIR) was found with a higher abundancy in patients in Grades II-IV than those in Grade I. 27 Here, the GEO GSE2223 dataset suggested that GABPB1-AS1 was highly expressed in glioma tissues. This trend was validated in the clinical samples, and this lncRNA was analyzed to show an ideal prognostic value in glioma and indicate increased glioma grades.…”

The HOTAIR lncRNA: A remarkable oncogenic promoter in human cancer metastasis (Review)

“…Collectively, these results suggest that HOTAIR may affect the potential of NSCLC cells to invade and migrate by altering the expression levels of MMPs and HOXA5 (72). In addition, Zhao et al (73) assessed 123 patients undergoing surgery for glioma and demonstrated that HOTAIR may facilitate invasion by increasing the expression levels of MMP-9, MMP-7 and VEGF. Kim et al reported that HOTAIR accelerates the invasiveness of cervical cancer cells by upregulating VEGF and MMP-9 expression (74).…”

Coding of Glioblastoma Progression and Therapy Resistance through Long Noncoding RNAs

“…Expression levels of HOTAIR > 0.40 had moderate predictive value for grades II-IV glioma. It promotes the invasion of gliomas through upregulation of the expression of matrix metalloproteinase-7 (MMP-7), matrix metalloproteinase-9 (MMP-9), and vascular endothelial growth factor (VEGF) [76]. Integrated in silico analysis, chromatin immunoprecipitation, and quantitative PCR data have shown that homeobox A9 (HOXA9) binds directly to the promoter of HOTAIR and might serve as a novel direct regulator of HOTAIR [77].…”