Association between circular RNA expression content and severity of coronary atherosclerosis in human coronary artery

Hou, Can; Gu, Lingfeng; Guo, Yesong; Zhou, Yaqing; Liu, Hua; Chen, Jiaxin; He, Shu; Zhang, Sheng; Jia, Qiaowei; Zhao, Cheng; Zhang, Jing; Xu, Guangxu; Jia, En-Zhi

doi:10.1002/jcla.23552

Cited by 15 publications

(9 citation statements)

References 23 publications

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“…CircRNAs are widely expressed in human cells and involved in transcriptional and posttranscriptional gene regulation, thereby regulating cell cycle, organ differentiation, and disease pathology. 20 The potential role of circRNAs in the process of cardiovascular diseases has also been reported. 21 Heart-related circRNA can act as miR-233 sponge and attenuates the development of heart failure.…”

Section: Discussionmentioning

confidence: 99%

Microarray profile of circular RNAs identifies hsa_circ_000455 as a new circular RNA biomarker for deep vein thrombosis

Lou

et al. 2021

Vascular

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Objects Deep vein thrombosis is a type of severe venous thromboembolism that can result in high mortality and morbidity. The expression alternation of circular RNAs (circRNAs) has been found in various diseases. However, the function of circRNAs in deep vein thrombosis still remains unknown. Method The blood samples of deep vein thrombosis patients and health control were selected, circRNA microarray was performed, and qPCR was used to verify the expression of circRNAs. Also, GO/KEGG analysis was performed, and hsa_circ_RNA_000455-targeted miRNA–mRNA network was predicted. Result Here, we found that 303 circRNAs were differentially expressed in deep vein thrombosis using microarray, of which 83 circRNAs were upregulated and 220 circRNAs were downregulated. The expression of five circRNAs verified by quantitative real-time PCR was consistent with the result of microarray. GO analysis showed that the top 100 differentially expressed circRNAs in deep vein thrombosis patients were closely related to protein transport, cytoplasm, and Adenosine Triphosphate (ATP) binding. The most significantly enriched pathways by KEGG analysis included thyroid hormone-signaling pathway, endocytosis, proteoglycans in cancer, Fc gamma R-mediated phagocytosis, focal adhesion, insulin-signaling pathway, p53-signaling pathway, biosynthesis of antibiotics, bacterial invasion of epithelial cells, and AMP-activated protein kinase-signaling pathway. Then, hsa_circ_000455 was selected, and the function of hsa_circ_000455 in the pathogenesis of deep vein thrombosis was analyzed via circRNA–miRNA–mRNA network. We therefore hypothesized that hsa_circRNA_000455/hsa-miR-22-3p/NLRP3 may involve in the development of deep vein thrombosis. Conclusion This study provided valuable information on circRNA profile in deep vein thrombosis for the first time and gave clues on the possible role and mechanism of hsa_circRNA_000455 in deep vein thrombosis.

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Section: Discussionmentioning

confidence: 99%

Microarray profile of circular RNAs identifies hsa_circ_000455 as a new circular RNA biomarker for deep vein thrombosis

Lou

et al. 2021

Vascular

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“…circRNAs were identified by a comprehensive literature search and the circRNA2Disease database [ 22 ]. In five separate experiments, a total of 116 circRNAs associated with CVD were obtained [ 23 , 24 , 25 , 26 , 27 ] ( Table S1 ). These circRNAs were individually submitted to a computational tool known as the “Circular RNA Interactome” that helps in the mapping and prediction of binding positions for particular miRNAs on reported circRNAs [ 28 ].…”

Section: Methodsmentioning

confidence: 99%

Competing Endogenous RNA Regulatory Networks of hsa_circ_0126672 in Pathophysiology of Coronary Heart Disease

Rafiq

Dandare

Javed

et al. 2023

Genes

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Coronary heart disease (CHD) is a global health concern, and its molecular origin is not fully elucidated. Dysregulation of ncRNAs has been linked to many metabolic and infectious diseases. This study aimed to explore the role of circRNAs in the pathogenesis of CHD and predicted a candidate circRNA that could be targeted for therapeutic approaches to the disease. circRNAs associated with CHD were identified and CHD gene expression profiles were obtained, and analyzed with GEO2R. In addition, differentially expressed miRNA target genes (miR-DEGs) were identified and subjected to functional enrichment analysis. Networks of circRNA/miRNA/mRNA and the miRNA/affected pathways were constructed. Furthermore, a miRNA/mRNA homology study was performed. We identified that hsa_circ_0126672 was strongly associated with the CHD pathology by competing for endogenous RNA (ceRNA) mechanisms. hsa_circ_0126672 characteristically sponges miR-145-5p, miR-186-5p, miR-548c-3p, miR-7-5p, miR-495-3p, miR-203a-3p, and miR-21. Up-regulation of has_circ_0126672 affected various CHD-related cellular functions, such as atherosclerosis, JAK/STAT, and Apelin signaling pathways. Our results also revealed a perfect and stable interaction for the hybrid of miR-145-5p with NOS1 and RPS6KB1. Finally, miR-145-5p had the highest degree of interaction with the validated small molecules. Henchashsa_circ_0126672 and target miRNAs, notably miR-145-5p, could be good candidates for the diagnosis and therapeutic approaches to CHD.

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“…Recent research shows that the profile expression of circRNAs is associated with different types of cardiovascular diseases such as cardiomyopathies, chronic heart failure, and coronary artery disease [ 53 , 54 , 55 , 56 , 57 , 58 , 59 ]. Jakobi et al [ 60 ] reported that circRNAs generated from Hectd1 , Ppp2r3a , Slc8a1 , Dmd , and Ttn host genes were associated with cardiomyopathies.…”

Section: Introductionmentioning

confidence: 99%

Circular RNAs: New Players in Cardiomyopathy

Moghaddam

Maleki

Oveisee

et al. 2022

Genes

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Cardiomyopathies comprise a heterogeneous group of cardiac diseases identified by myocardium disorders and diminished cardiac function. They often lead to heart failure or heart transplantation and constitute one of the principal causes of morbidity and mortality worldwide. Circular RNAs (circRNAs) are a novel type of noncoding RNAs. They are covalently closed and single-stranded and derived from the exons and introns of genes by alternative splicing. This specific structure renders them resistant to exonuclease digestion. Many recent studies have demonstrated that circRNAs are highly abundant and conserved and can play central roles in biological functions such as microRNA (miRNA) sponging, splicing, and transcription regulation. Emerging evidence indicates that circRNAs can play significant roles in cardiovascular diseases, including cardiomyopathies. In this review, we briefly describe the current understanding regarding the classification, nomenclature, characteristics, and function of circRNAs and report recent significant findings concerning the roles of circRNAs in cardiomyopathies. Furthermore, we discuss the clinical application potential of circRNAs as the therapeutic targets and diagnostic biomarkers of cardiomyopathies.

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Association between circular RNA expression content and severity of coronary atherosclerosis in human coronary artery

Abstract: This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

Cited by 15 publications

References 23 publications

Microarray profile of circular RNAs identifies hsa_circ_000455 as a new circular RNA biomarker for deep vein thrombosis

Microarray profile of circular RNAs identifies hsa_circ_000455 as a new circular RNA biomarker for deep vein thrombosis

Competing Endogenous RNA Regulatory Networks of hsa_circ_0126672 in Pathophysiology of Coronary Heart Disease

Circular RNAs: New Players in Cardiomyopathy

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