Association between Brain and Plasma Glutamine Levels in Healthy Young Subjects Investigated by MRS and LC/MS

Takado, Yuhei; Sato, Naoto; Kanbe, Yuta; Tomiyasu, Moyoko; Xin, Lijing; Near, Jamie; Yoshikawa, Kohki; Sahara, Naruhiko; Higashi, Tatsuya; Suhara, Tetsuya; Higuchi, Makoto; Obata, Takayuki

doi:10.3390/nu11071649

Cited by 22 publications

(17 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Glutamine, an important glutamatergic metabolite, and its concentrations in the posterior cingulate cortex of the brain, have shown a positive correlation with its concentrations in plasma when measured with in vivo magnetic resonance spectroscopy (MRS) [11]. Largescale genome-wide association study (GWAS) data have revealed a protective effect of circulating glutamine against AD, suggesting that more circulating glutamine might result in more available substrates during times of stress and act as a neuroprotectant [12].…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, unlike MS, NMR requires less sample preparation and has the potential to be implemented in in vivo observations with widely available MRI scanners. Using NMR and MS, AD metabolism and metabolomics have investigated human biofluids ranging from cerebrospinal fluid [1][2][3][4][5][6], to less-invasive blood [7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25], to entirely non-invasive saliva and urine [26,27].…”

mentioning

confidence: 99%

See 1 more Smart Citation

A Nuclear Magnetic Resonance Spectroscopy Method in Characterization of Blood Metabolomics for Alzheimer’s Disease

et al. 2022

View full text Add to dashboard Cite

There is currently a crucial need for improved diagnostic techniques and targeted treatment methods for Alzheimer’s disease (AD), a disease which impacts millions of elderly individuals each year. Metabolomic analysis has been proposed as a potential methodology to better investigate and understand the progression of this disease. In this report, we present our AD metabolomics results measured with high resolution magic angle spinning (HRMAS) nuclear magnetic resonance (NMR) on human blood plasma samples obtained from AD and non-AD subjects. Our study centers on developments of AD and non-AD metabolomics differentiating models with procedures of quality assurance (QA) and quality control (QC) through pooled samples. Our findings suggest that analysis of blood plasma samples using HRMAS NMR has the potential to differentiate between diseased and healthy subjects, which has important clinical implications for future improvements in AD diagnosis methodologies.

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

A Nuclear Magnetic Resonance Spectroscopy Method in Characterization of Blood Metabolomics for Alzheimer’s Disease

et al. 2022

View full text Add to dashboard Cite

show abstract

“…55,56 Because of the blood-brain barrier, changes in plasma concentrations of neurochemicals do not correlate with cerebral concentrations in healthy individuals. 57 However, with the disruption of the blood-brain barrier in CKD, higher serum concentrations could theoretically affect cerebral neurochemical concentrations. Our study indicates normalization of Cho and mI post-KT, perhaps by better elimination with tubular secretion, 52,53 which cannot be restored with dialysis or measured by creatinine clearance or serum creatinine-based eGFR.…”

Section: Discussionmentioning

confidence: 99%

Normalization of Cerebral Blood Flow, Neurochemicals, and White Matter Integrity after Kidney Transplantation

Lepping¹,

Montgomery²,

Sharma³

et al. 2020

JASN

View full text Add to dashboard Cite

BackgroundCKD is associated with abnormalities in cerebral blood flow, cerebral neurochemical concentrations, and white matter integrity. Each of these is associated with adverse clinical consequences in the non-CKD population, which may explain the high prevalence of dementia and stroke in ESKD. Because cognition improves after kidney transplantation, comparing these brain abnormalities before and after kidney transplantation may identify potential reversibility in ESKD-associated brain abnormalities.MethodsIn this study of patients with ESKD and age-matched healthy controls, we used arterial spin labeling to assess the effects of kidney transplantation on cerebral blood flow and magnetic resonance spectroscopic imaging to measure cerebral neurochemical concentrations (N-acetylaspartate, choline, glutamate, glutamine, myo-inositol, and total creatine). We also assessed white matter integrity measured by fractional anisotropy (FA) and mean diffusivity (MD) with diffusion tensor imaging. We used a linear mixed model analysis to compare longitudinal, repeated brain magnetic resonance imaging measurements before, 3 months after, and 12 months after transplantation and compared these findings with those of healthy controls.ResultsStudy participants included 29 patients with ESKD and 19 controls; 22 patients completed post-transplant magnetic resonance imaging. Cerebral blood flow, which was higher in patients pretransplant compared with controls (P=0.003), decreased post-transplant (P<0.001) to values in controls. Concentrations of neurochemicals choline and myo-inositol that were higher pretransplant compared with controls (P=0.001 and P<0.001, respectively) also normalized post-transplant (P<0.001 and P<0.001, respectively). FA increased (P=0.001) and MD decreased (P<0.001) post-transplant.ConclusionsCertain brain abnormalities in CKD are reversible and normalize with kidney transplantation. Further studies are needed to understand the mechanisms underlying these brain abnormalities and to explore interventions to mitigate them even in patients who cannot be transplanted.Clinical Trial registry name and registration number:Cognitive Impairment and Imaging Correlates in End Stage Renal Disease, NCT01883349

show abstract

“…We previously examined the glutamate concentration in the vitreous, but it was not increased in GLAST +/- mice [ 3 ]. We tried to detect glutamate concentration using magnetic resonance spectroscopy i n vivo [ 18 , 19 ], but failed because mouse eyes were too small for the methods. We would like to determine the possibility of increased glutamate levels in the mouse eye in the future experiments.…”

Section: Discussionmentioning

confidence: 99%

Effects of lighting environment on the degeneration of retinal ganglion cells in glutamate/aspartate transporter deficient mice, a mouse model of normal tension glaucoma

Ōhashi

Namekata

Guo

et al. 2022

Biochemistry and Biophysics Reports

View full text Add to dashboard Cite

Lighting conditions may affect the development of retinal degenerative diseases such as macular degeneration. In this study, to determine whether the lighting environment affects the progression of degeneration of retinal ganglion cells (RGCs), we examined glutamate/aspartate transporter (GLAST) heterozygous (GLAST +/- ) mice, a mouse model of normal tension glaucoma. GLAST +/- mice were reared under a 12-h light-dark cycle (Light/Dark) or complete darkness (Dark/Dark) condition after birth. The total RGC number in the Dark/Dark group was significantly decreased compared with the Light/Dark group at 3 weeks old, while the number of osteopontin-positive αRGCs were similar in both groups. At 6 and 12 weeks old, the total RGC number were not significantly different in both conditions. In addition, the retinal function examined by multifocal electroretinogram were similar at 12 weeks old. These results suggest that lighting conditions may regulate the progression of RGC degeneration in some types of glaucoma.

show abstract

Association between Brain and Plasma Glutamine Levels in Healthy Young Subjects Investigated by MRS and LC/MS

Cited by 22 publications

References 45 publications

A Nuclear Magnetic Resonance Spectroscopy Method in Characterization of Blood Metabolomics for Alzheimer’s Disease

A Nuclear Magnetic Resonance Spectroscopy Method in Characterization of Blood Metabolomics for Alzheimer’s Disease

Normalization of Cerebral Blood Flow, Neurochemicals, and White Matter Integrity after Kidney Transplantation

Effects of lighting environment on the degeneration of retinal ganglion cells in glutamate/aspartate transporter deficient mice, a mouse model of normal tension glaucoma

Contact Info

Product

Resources

About