Association Between Antinuclear Antibodies and the HLA Class II Locus and Heterogeneous Characteristics of Staining Patterns: The Nagahama Study

Terao, Chikashi; Ohmura, Koichiro; Yamada, Ryo; Kawaguchi, Takahisa; Shimizu, Masakazu; Tabara, Yasuharu; Takahashi, Meiko; Setoh, Kazuya; Nakayama, Takeo; Kosugi, Shinji; Sekine, Akihiro; Matsuda, Fumihiko; Mimori, Tsuneyo

doi:10.1002/art.38867

Cited by 18 publications

(27 citation statements)

References 39 publications

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“…In the majority of our cases (93%) the staining pattern was stable over time, but in four patients the pattern changed at least once. Nevertheless, the distribution herein was largely similar to that found in a previous Swedish SLE cohort, but with a higher observed proportion of H‐ANA‐positive individuals compared to both a large Japanese study with volunteers where S‐ANA constituted the dominating staining pattern and in a Mexican report which evaluated relatives of patients with autoimmune disease .…”

Section: Discussionsupporting

confidence: 85%

Longitudinal anti-nuclear antibody (ANA) seroconversion in systemic lupus erythematosus: a prospective study of Swedish cases with recent-onset disease

Frodlund

Wetterö

Dahle

et al. 2019

Clinical &Amp; Experimental Immunology

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Summary Serum immunoglobulin (Ig)G anti‐nuclear antibodies (ANA) detected by indirect immunofluorescence (IF) microscopy remains a hallmark of systemic lupus erythematosus (SLE). Whether or not IF‐ANA status varies over time is controversial. We therefore designed a prospective study with longitudinal follow‐up of patients with recent‐onset SLE. The study population consisted of 54 recently diagnosed SLE cases, all meeting the 1982 American College of Rheumatology (ACR) and/or the 2012 Systemic Lupus International Collaborating Clinics (SLICC) criteria. Clinical follow‐up data, including disease activity, organ damage and sera, were collected from clinical onset of SLE and onwards, in most cases yearly (0‒96 months). IF‐ANA was analysed on human epithelial cells‐2 (HEp‐2) cells and categorized regarding staining patterns. Using an addressable laser bead assay (FIDIS™ Connective profile), we measured IgG‐ANA fine specificities against Ro52/SSA, Ro60/SSA, Sjögren’s syndrome type B antigen (La/SSB), Smith antigen (Sm), Smith antigen/ribonucleoprotein (Sm/RNP), U1 RNP (U1RNP), dsDNA, ribosomal‐P protein and histone. At baseline, all patients were judged ANA‐positive at an abnormal titre corresponding to the 95th percentile of healthy blood donors, but seven of 54 patients (13%) lost ANA‐positivity over time. Homogeneous (AC‐1; 46%) and speckled (AC‐4 or 5; 31%) were the most frequently observed patterns at inclusion, whereas 7% switched pattern at least once during follow‐up. Established associations between ANA fine specificities and clinical data were confirmed. Levels of anti‐Sm/RNP, but not of anti‐dsDNA, correlated with clinical disease activity [modified SLE disease activity 2000 (mSLEDAI‐2K)]. Our data indicate that a considerable proportion of Swedish patients with SLE lose ANA‐positivity over time, whereas consistent staining patterns were frequent. The clinical and mechanistic relevance of ANA seroconversion remains uncertain. Further prospective evaluations in larger SLE populations with more diverse ethnicities are warranted.

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Section: Discussionsupporting

confidence: 85%

Longitudinal anti-nuclear antibody (ANA) seroconversion in systemic lupus erythematosus: a prospective study of Swedish cases with recent-onset disease

Frodlund

Wetterö

Dahle

et al. 2019

Clinical &Amp; Experimental Immunology

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“…While ANAs are observed in healthy populations as well as in patients with autoimmune diseases such as SLE, one genetic study was performed in Japanese participants that quantified ANA levels using indirect immunofluorescence to determine any correlation of ANA positivity with a genetic locus. The results showed that the nucleolar pattern showed different associations from other staining patterns, correlating with ANA positivity and with the HLA locus 8 . In this study, ANA positivity in Japanese volunteers ages range 30-75 years with low titres were found between 12.5 and 45.2%, and medium titres were found only 2.8%.…”

Section: Discussionmentioning

confidence: 86%

Serum antinuclear antibody in adult Thais

et al. 2016

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“…Titers above 1:40 were considered positive in the EAC. Titers above 1:80 were considered positive in the Nagahama study [28]. …”

Section: Methodsmentioning

confidence: 99%

Smoking is associated with the concurrent presence of multiple autoantibodies in rheumatoid arthritis rather than with anti-citrullinated protein antibodies per se: a multicenter cohort study

Wesemael¹,

Ajeganova²,

Humphreys³

et al. 2016

Arthritis Res Ther

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BackgroundThe contribution of smoking to rheumatoid arthritis (RA) is hypothesized to be mediated through formation of anti-citrullinated protein antibodies (ACPA). In RA, however, autoantibodies such as ACPA, rheumatoid factor (RF), and anti-carbamylated protein antibodies (anti-CarP) often occur together, and it is thus unclear whether smoking is specifically associated with some autoantibodies rather than others. We therefore investigated whether smoking is only associated with ACPA or with the presence of multiple RA-related autoantibodies.MethodsA population-based Japanese cohort (n = 9575) was used to investigate the association of smoking with RF and anti-cyclic citrullinated peptide antibodies (anti-CCP2) in individuals without RA. Furthermore, RA patients fulfilling the 1987 criteria from three early arthritis cohorts from the Netherlands (n = 678), the United Kingdom (n = 761), and Sweden (n = 795) were used. Data on smoking, RF, anti-CCP2, and anti-CarP were available. A total score of autoantibodies was calculated, and odds ratios (ORs) and 95% confidence intervals (95% CIs) were calculated by logistic regression.ResultsIn the population-based non-RA cohort, no association was found between smoking and one autoantibody (RF or anti-CCP2), but smoking was associated with double-autoantibody positivity (OR 2.95, 95% CI 1.32–6.58).In RA patients, there was no association between smoking and the presence of one autoantibody (OR 0.99, 95% CI 0.78–1.26), but smoking was associated with double-autoantibody positivity (OR 1.32, 95% CI 1.04–1.68) and triple-autoantibody positivity (OR 2.05, 95% CI 1.53–2.73).ConclusionsSmoking is associated with the concurrent presence of multiple RA-associated autoantibodies rather than just ACPA. This indicates that smoking is a risk factor for breaking tolerance to multiple autoantigens in RA.Electronic supplementary materialThe online version of this article (doi:10.1186/s13075-016-1177-9) contains supplementary material, which is available to authorized users.

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Association Between Antinuclear Antibodies and the HLA Class II Locus and Heterogeneous Characteristics of Staining Patterns: The Nagahama Study

Cited by 18 publications

References 39 publications

Longitudinal anti-nuclear antibody (ANA) seroconversion in systemic lupus erythematosus: a prospective study of Swedish cases with recent-onset disease

Longitudinal anti-nuclear antibody (ANA) seroconversion in systemic lupus erythematosus: a prospective study of Swedish cases with recent-onset disease

Serum antinuclear antibody in adult Thais

Smoking is associated with the concurrent presence of multiple autoantibodies in rheumatoid arthritis rather than with anti-citrullinated protein antibodies per se: a multicenter cohort study

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