Association between algal productivity and phycosphere composition in an outdoor <i>Chlorella sorokiniana</i> reactor based on multiple longitudinal analyses

Steichen, Seth A.; Gao, Song; Waller, Peter; Brown, Judith K.

doi:10.1111/1751-7915.13591

Cited by 18 publications

(10 citation statements)

References 80 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The members of this family are highly versatile, interacting with animals, humans, plants and fungi ( Bontemps et al, 2010 ; Hoffman and Arnold, 2010 ). They have been associated with micro -and macro-algae bacterial communities ( Hurtado-McCormick et al, 2019 ; Steichen et al, 2020 ; Qu et al, 2021 ; Yu et al, 2022 ). These bacteria can fix N2 providing nutrients for the algae host ( Bontemps et al, 2010 ; Gyaneshwar et al, 2011 ).…”

Section: Discussionmentioning

confidence: 99%

The microbiome of the dinoflagellate Prorocentrum cordatum in laboratory culture and its changes at higher temperatures

Sanchez-Garcia

Wang²,

Wagner‐Döbler³

2022

Front. Microbiol.

View full text Add to dashboard Cite

In the ocean, phytoplankton are dependent on communities of bacteria living in the phycosphere, a hot spot of metabolic and genetic exchange. Many types of interactions between phytoplankton and phycosphere bacteria have been shown, but it is unclear if the microbial communities associated with microalgae strains in culture collections are beneficial or harmful to the host strain. Here, we studied the microbial communities associated with four strains of the dinoflagellate Prorocentrum cordatum that had been isolated from distant geographical locations and maintained in culture collection for hundreds of generations. Community composition was determined by 16S rRNA gene amplicon sequencing. The dinoflagellate host strain was the strongest parameter separating communities, while growth phase, lifestyle (particle-attached versus free-living) and temperature had only a modulating effect. Although the strains had been isolated from distant locations in the Atlantic and Pacific Ocean, 14 ASVs were shared among all strains, the most abundant ones being Gilvibacter, Marivita, uncultivated Rhodobacteraceae, Marinobacter, Hyphomonadaceae, Cupriavidus, Variovorax, and Paucibacter. Adaptation to higher temperatures resulted in specific changes in each phycosphere microbiome, including increased abundance of rare community members. We then compared the growth of the four xenic cultures to that of the axenic P. cordatum CCMP1329. At 20°C, growth of the xenic cultures was similar or slower than that of CCMP1329. At 26°C, all four xenic cultures experienced a death phase, while the axenic culture stably remained in the stationary phase. At 30°C, only two of the xenic cultures were able to grow. A shift of dinoflagellate metabolism from autotrophy to mixotrophy and competition between dinoflagellate and bacteria for limiting nutrients, including essential vitamins, may contribute to these differences in growth patterns.

show abstract

Section: Discussionmentioning

confidence: 99%

The microbiome of the dinoflagellate Prorocentrum cordatum in laboratory culture and its changes at higher temperatures

Sanchez-Garcia

Wang²,

Wagner‐Döbler³

2022

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Besides, bacteria that are used for bioremediation processes include members of the genera Pseudomonas , Sphingomonas , Chryseobacterium , as well as Flavobacterium [ 51 ]. Most of these bacteria were also detected as main representatives of microbial communities in other algae–bacteria systems that grew in other wastewater systems [ 52 ] and freshwater reactors [ 53 ]. Interestingly, representatives of the orders Rhizobiales , Betaproteobacteriales , and Chitinophagales correlated with biomass productivity of C. sorokiniana strain DOE1412 during outdoor cultivation [ 53 ].…”

Section: Resultsmentioning

confidence: 99%

Assessment of Chlorella sorokiniana Growth in Anaerobic Digester Effluent

Ziganshina

Bulynina

Ziganshin

2021

Plants

View full text Add to dashboard Cite

Microalgae are considered a potential source of valuable compounds for multiple purposes and are potential agents for bioremediation of aquatic environments contaminated with different pollutants. This work evaluates the use of agricultural waste, unsterilized and anaerobically digested, to produce biomass from a strain of Chlorella sorokiniana. Furthermore, the presence of bacteria in these wastes was investigated based on the bacterial 16S rRNA gene sequencing. The results showed a specific growth rate ranging between 0.82 and 1.45 day–1, while the final biomass yield in different digestate-containing treatments (bacterial-contaminated cultures) ranged between 0.33 and 0.50 g L−1 day−1. Besides, substantial amounts of ammonium, phosphate, and sulfate were consumed by C. sorokiniana during the experimental period. The predominant bacteria that grew in the presence of C. sorokiniana in the effluent-containing treatments belonged to the genera Chryseobacterium, Flavobacterium, Sphingomonas, Brevundimonas, Hydrogenophaga, Sphingobacterium, and Pseudomonas. Therefore, this microalga can tolerate and grow in the presence of other microorganisms. Finally, these results show that anaerobically digested agricultural waste materials are a good substitute for growth media for green microalgae; however, phosphate and sulfate levels must also be controlled in the media to maintain adequate growth of microalgae.

show abstract

“…For instance, bacterial sporulation, such as that reported for species of the order Bacillales , increases the level of dye uptake, resulting in higher emission on the respective channel ( Zhang et al, 2020 ). Members affiliated with the order of Bacillales were also reported for microalgal cultures ( Steichen et al, 2020 ). To study those interactions, fluorescence-activated cell sorting would allow separating the different prokaryotic populations of interest.…”

Section: Discussionmentioning

confidence: 99%

Automated Online Flow Cytometry Advances Microalgal Ecosystem Management as in situ, High-Temporal Resolution Monitoring Tool

Haberkorn

Off

Besmer³

et al. 2021

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Microalgae are emerging as a next-generation biotechnological production system in the pharmaceutical, biofuel, and food domain. The economization of microalgal biorefineries remains a main target, where culture contamination and prokaryotic upsurge are main bottlenecks to impair culture stability, reproducibility, and consequently productivity. Automated online flow cytometry (FCM) is gaining momentum as bioprocess optimization tool, as it allows for spatial and temporal landscaping, real-time investigations of rapid microbial processes, and the assessment of intrinsic cell features. So far, automated online FCM has not been applied to microalgal ecosystems but poses a powerful technology for improving the feasibility of microalgal feedstock production through in situ, real-time, high-temporal resolution monitoring. The study lays the foundations for an application of automated online FCM implying far-reaching applications to impel and facilitate the implementation of innovations targeting at microalgal bioprocesses optimization. It shows that emissions collected on the FL1/FL3 fluorescent channels, harnessing nucleic acid staining and chlorophyll autofluorescence, enable a simultaneous assessment (quantitative and diversity-related) of prokaryotes and industrially relevant phototrophic Chlorella vulgaris in mixed ecosystems of different complexity over a broad concentration range (2.2–1,002.4 cells ⋅μL–1). Automated online FCM combined with data analysis relying on phenotypic fingerprinting poses a powerful tool for quantitative and diversity-related population dynamics monitoring. Quantitative data assessment showed that prokaryotic growth phases in engineered and natural ecosystems were characterized by different growth speeds and distinct peaks. Diversity-related population monitoring based on phenotypic fingerprinting indicated that prokaryotic upsurge in mixed cultures was governed by the dominance of single prokaryotic species. Automated online FCM is a powerful tool for microalgal bioprocess optimization owing to its adaptability to myriad phenotypic assays and its compatibility with various cultivation systems. This allows advancing bioprocesses associated with both microalgal biomass and compound production. Hence, automated online FCM poses a viable tool with applications across multiple domains within the biobased sector relying on single cell–based value chains.

show abstract

Association between algal productivity and phycosphere composition in an outdoor Chlorella sorokiniana reactor based on multiple longitudinal analyses

Cited by 18 publications

References 80 publications

The microbiome of the dinoflagellate Prorocentrum cordatum in laboratory culture and its changes at higher temperatures

The microbiome of the dinoflagellate Prorocentrum cordatum in laboratory culture and its changes at higher temperatures

Assessment of Chlorella sorokiniana Growth in Anaerobic Digester Effluent

Automated Online Flow Cytometry Advances Microalgal Ecosystem Management as in situ, High-Temporal Resolution Monitoring Tool

Contact Info

Product

Resources

About