Association Between -1562C/T Polymorphisms in the Matrix Metalloproteinase-9 and the Risk of Lung Cancer Among South-Central Chinese Population

Lu, Li-jie; Li, Wen; Liu, Cun; Jia, Mingxi; Xing, Fu-qi; Jiang, Pei; Tang, Jianxin

doi:10.1166/jbns.2016.1382

Cited by 2 publications

(6 citation statements)

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“…Moreover, the serum levels of MMP13 were detected between NSCLC and controls. Some scientific researchers reported that high expression of MMP13 was associated with NSCLC infiltration, metastasis, and recurrence [12,17,19,26,31]. The G allele of MMP13 showed a higher expression than the A allele in our experiment.…”

Section: Discussionsupporting

confidence: 47%

“…Contrary to these findings, a recent case–control study and a minireview by Banday et al found that the CT heterozygous genotype of MMP9-1562C/T SNP showed a significant association with increased risk for the development of colorectal cancer in Kashmiri population, compared with T allele [5]. Differently, our previous studies found that compared with the CT genotype, the MMP9-1562CC genotype might be a marker of increased genotype susceptibility to NSCLC among the South-Central Chinese population [19]. Although the genotypes were evenly distributed between NSCLC patients and healthy individuals in current research, no difference were founded between MMP9 polymorphisms and NSCLC with histological type, clinical stage, lymph node metastasis parameters ( p > 0.05).…”

Section: Discussionmentioning

confidence: 99%

“…The C allele of MMP9 showed a higher expression than the T allele. Our dataset further proved that the serum levels of MMP-9 with NSCLC were compared with the obvious increase in the control group ( p < 0.05), the C allele of MMP-9 showed a higher expression s than in T allele, the serum levels of MMP-9 in peripheral blood can reflect the load state of the body, and a large number of scientific studies have shown that high expression of MMP-9 is closely related to tumorigenesis, invasion and metastasis, and patient prognosis [12,17,19,26], and there is a correlation between genetic findings and enzymatic activity, the results demonstrated that C allele of MMP9-1562C/T could increase serum level of NSCLC compared with the A allele (OR = 1.19, 95% CI = 0.75–1.89) and CC genotype, and the C allele could increase the risk of lung cancer, which was consistent with the results of the SNP analysis.…”

Section: Discussionmentioning

confidence: 99%

“…Our data demonstrated evident compliance between genetic findings and enzymatic activity; however this correlation is still only a trend. Because the occurrence and development of tumors are affected by many factors [12,17,19,31], and the single-nucleotide polymorphism (SNP) depends on the genetic background of the individual as well as environmental factors, a limitation of the present study was the small number of patients with each category of gene type, which may affect the estimates of results for genotype, and some further scientific researchers should be needed in future.…”

Section: Discussionmentioning

confidence: 99%

“…The polymorphism in MMP13 was determined by PCR-RFLP, the oligonucleotide primers used for amplification of the specific promoter region containing the MMP13 -77A/G site were 5′-GATACGTTCTTACAGAAGGC-3′ (Forward) and 5′-GACAAATCATCTTCATCACC-3′ (Reverse) [19]. An initial denaturation step at 95 °C for 2 min was followed by 40 cycles of 94 °C for 30 s, 64 °C for 30 s, 72 °C for 30 s, and a final extension step at 72 °C for 10 min.…”

Section: Methodsmentioning

confidence: 99%

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Association of MMP9-1562C/T and MMP13-77A/G Polymorphisms with Non-Small Cell Lung Cancer in Southern Chinese Population

Jia

Wang

et al. 2019

Biomolecules

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Background: Matrix metalloproteinases (MMPs) are capable of degrading and modifying most components of the extracellular matrix (ECM) and the basal membrane (BM), and play crucial roles in cancer invasion and metastasis. MMP gene expressions were regulated primarily at the transcriptional level, which was associated with tumor spread and patient prognosis. Polymorphisms in MMPs have been reported to be associated with non-small cell lung cancer (NSCLC). The objective of this study aim to evaluate the serum levels and polymorphisms of MMP-9 and MMP-13 in non-small cell lung cancer patients compared to normal subjects and their correlation to non-small cell lung cancer histopathology findings in Southern Chinese people. Methods: This case–control study included 245 patients with NSCLC and 258 healthy controls. Genomic DNA was extracted by using DNA extraction kit, genotyping was confirmed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and direct DNA sequencing, and serum levels of MMP-9 and MMP-13 were measured by using a specific ELISA, Human Matrix Metalloproteinase Enzyme Immunoassay Kits. Statistical analysis was carried out using the SPSS 23.0 software package. Results: The subjects carrying the TT genotype had a decreased risk of lung cancer in MMP9-1562C/T comparing with the CC genotype (p = 0.00, OR = 0.45, 95% CI = 0.29–0.68), and the MMP13-77 AA genotype was associated with a decreased risk of NSCLC by comparing with the GG genotype (p = 0.03, OR = 0.56, 95% CI = 0.33–0.94). Moreover, the C allele of MMP9-1562C/T could increase serum level of NSCLC in compared with the A allele (OR = 1.19, 95% CI = 0.75–1.89). Similarly, the AA genotype of MMP13 might be a marker of decreased serum level of lung cancer (OR = 0.76, 95% CI = 0.51–1.14). Conclusions: The results of these analyses underline the support of the notion that the CC genotype of MMP9-1562C/T and GG genotypes of MMP13-77G/A were associated with the increased risk NSCLC, and the serum levels of MMP9 and MMP13 were consistent with the results of the SNP analysis. MMP13 and MMP9 might be function as a key oncogene in NSCLC with a Southern Chinese population. Combined detection of SNP and enzyme activity between MMP9 and MMP13 are expected to be a potential diagnostic method of non-small cell lung cancer.

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Section: Discussionsupporting

confidence: 47%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Association of MMP9-1562C/T and MMP13-77A/G Polymorphisms with Non-Small Cell Lung Cancer in Southern Chinese Population

Jia

Wang

et al. 2019

Biomolecules

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Role of matrix metalloproteinase 9 gene polymorphism as genetic risk factor for lung cancer

Rashid

Al-Husseini

2022

ijhs

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Lung cancer (LC) is one of the most incident malignancies worldwide. This study investigated the potential role of matrix metalloproteinase9 gene (MMP9) single nucleotide polymorphisms (SNPs) in Lung cancer patients. The study population was included 75 Iraqi patients with confirmed Lung cancer and 25 healthy people as controls. In this study, the -1562 C/T polymorphism in MMP9 gene was detected by restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) technique. The genotype distribution results of the -1562 C/T SNP of MMP9 gene have shown no significant differences (p>0.05) between controls (CC: n= 15, 60%; CT: n=9, 36%; TT: n=1, 4%) and Lung cancer patients (CC: n= 44, 58.66%; CT: n=30, 40%; TT: n=1, 1.34%). Results of the association analysis between genotype distribution of the -1562 C/T SNP of MMP9 gene and clinicopathological criteria of cases such as histological grading, TNM staging and lymph node metastasis, had shown no significant association (p>0.05). While analysis of the association between genotype distribution of the -1562 C/T SNP of MMP9 gene and smoking status among patients were statically significant (p<0.05). These results suggested that the SNP in MMP9 gene was involved in lung cancer progression, tissue invasion and metastasis involvement.

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Association Between -1562C/T Polymorphisms in the Matrix Metalloproteinase-9 and the Risk of Lung Cancer Among South-Central Chinese Population

Cited by 2 publications

References 0 publications

Association of MMP9-1562C/T and MMP13-77A/G Polymorphisms with Non-Small Cell Lung Cancer in Southern Chinese Population

Association of MMP9-1562C/T and MMP13-77A/G Polymorphisms with Non-Small Cell Lung Cancer in Southern Chinese Population

Role of matrix metalloproteinase 9 gene polymorphism as genetic risk factor for lung cancer

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