Associated SNPs, Heritabilities, Trait Correlations, and Genomic Breeding Values for Resistance in Snap Beans (Phaseolus vulgaris L.) to Root Rot Caused by Fusarium solani (Mart.) f. sp. phaseoli (Burkholder)

Abstract

Root rot is a major constraint to snap bean (Phaseolus vulgaris) production in the United States and around the world. Genetic resistance is needed to effectively control root rot disease because cultural control methods are ineffective, and the pathogen will be present at the end of one season of production on previously clean land. A diversity panel of 149 snap bean pure lines was evaluated for resistance to Fusarium root rot in Oregon. Morphological traits potentially associated with root rot resistance, such as aboveground biomass, adventitious roots, taproot diameter, basal root diameter, deepest root angle, shallowest root angle, root angle average, root angle difference, and root angle geometric mean were evaluated and correlated to disease severity. A genome wide association study (GWAS) using the Fixed and random model Circulating Probability Unification (FarmCPU) statistical method, identified five associated single nucleotide polymorphisms (SNPs) for disease severity and two SNPs for biomass. The SNPs were found on Pv03, Pv07, Pv08, Pv10, and Pv11. One candidate gene for disease reaction near a SNP on Pv03 codes for a peroxidase, and two candidates associated with biomass SNPs were a 2-alkenal reductase gene cluster on Pv10 and a Pentatricopeptide repeat domain on Pv11. Bean lines utilized in the study were ranked by genomic estimated breeding values (GEBV) for disease severity, biomass, and the root architecture traits, and the observed and predicted values had high to moderate correlations. Cross validation of genomic predictions showed slightly lower correlational accuracy. Bean lines with the highest GEBV were among the most resistant, but did not necessarily rank at the very top numerically. This study provides information on the relationship of root architecture traits to root rot disease reaction. Snap bean lines with genetic merit for genomic selection were identified and may be utilized in future breeding efforts.

A robust SNP-haplotype assay for Bct gene region conferring resistance to beet curly top virus in common bean (Phaseolus vulgaris L.)

“…The SnAP expands on the 149 snap bean accessions for the Snap Bean Diversity Panel (SBDP) that composed part of the BeanCAP (Common Bean Coordinated Agricultural Project) panel, which was comprised of both dry and snap beans (http:// arsftfbean.uprm.edu/beancap/). The SBDP was used in a diversity analysis of snap bean (Wallace et al, 2018) and for GWAS of total phenolic content and root rot resistance (Myers et al, 2019;Huster et al, 2021). In addition to the SnAP, a dry bean population (DBP) comprised of 88 advanced inbred breeding lines and cultivars representing different market classes was included from the USDA-ARS bean breeding program in Prosser, WA.…”

Influence of organic plant breeding on the rhizosphere microbiome of common bean (Phaseolus vulgaris L.)

“…The elite parent Hystyle has resistance to Bean Common Mosaic Virus (BCMV), Bacterial Brown Spot, and Curly Top Virus, while the elite parent OR5630 has resistance to BCMV, alone (Cornell University, 2012). The older cultivar Provider is resistant to BCMV, while the older cultivar Black Valentine was recently reported to have some resistance to the root rot complex found in western Oregon (Huster et al, 2021).…”

“…Given the inherent disease resistance of OR5630, and other studies which used Black Valentine as a disease-susceptible control, it is possible that some of this shift could be attributed to this less resilient parent (Scott and Fulton, 1978;Pflieger et al, 2014). However, previous research into the root rot susceptibility of the four parental lines used in the production of the RIL population determined that Black Valentine has high resistance to the specific root rot complex prevalent in western Oregon (Huster et al, 2021).…”

Optimalisasi Populasi Tanaman pada Varietas Kacang Buncis (Phaseolus vulgaris L.) Crindo 19

“…Kacang buncis bertipe tumbuh merambat lebih peka terhadap tekanan kekeringan (stress air) dan suhu tinggi dibanding kacang buncis bertipe tegak karena kacang buncis bertipe merambat biasanya memiliki pajang tanaman yang lebih tinggi dan jumlah daun yang lebih banyak. Sementara itu, pertumbuhan dan perkembangan kacang buncis dipengaruhi oleh faktor genetik dan faktor lingkungan seperti pH, suhu, kelembaban dan ketersediaan unsur hara (Arfarita et al, 2020;Huster et al, 2021). Demikian juga, produksi polong tanaman buncis dipengaruhi oleh genotipe dan lingkungan tumbuh, seperti adanya perbedaan dalam kesuburan tanah dan cuaca (Arfarita et al, 2020).…”