Assisted Reproductive Technologies and Embryo Culture Methods for Farm Animals

Godke, R.A.; Sansinena, M.; Youngs, Curtis R.

doi:10.1016/b978-0-12-410490-7.00022-0

Cited by 9 publications

(5 citation statements)

References 259 publications

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“…On the other hand, the age of the oocyte influences the tolerance of embryos to in vitro manipulation [ 10 ]. Bisection and in vitro culture allow the identification of viable demi-embryos to increase the number of available embryos [ 1 ]. In the present study, the total rate of viable reconstituted demi-embryos was 139.5% of the manipulated blastocysts, which agrees with 152% reported by Hashiyada [ 2 ] in cattle.…”

Section: Discussionmentioning

confidence: 99%

“…In vitro embryo production (IVEP) has been implemented in genetic improvement programs and basic research on animal reproduction. Blastocysts can be split to increase the number of viable demi-embryos for transfer to recipient females and increase the number of offspring [ 1 ]. On the other hand, the efficiency of embryo bisection is affected by the quality and origin of the whole embryo [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

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In vitro embryo production from ewes at different physiological stages

et al. 2023

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Background The collection of ovaries from slaughterhouses is an important source of oocytes for in vitro embryo production. On the other hand, the physiological stage of slaughtered females varies and influences embryo production. Objectives The study examined the in vitro efficiency of embryos and demi-embryos from young, non-pregnant adult, and pregnant adult ewes from a local slaughterhouse. Methods One thousand three hundred ovaries were collected from August to October 2020. The recovered oocytes were matured, fertilized, and cultured at 5% CO 2 , 38.5°C, and 100% humidity. Embryo bisection was performed in 96 blastocysts (n = 32 per treatment). The demi-embryo pairs were incubated for their reconstitution for 12 h. SAS was used for data analysis. Results The number of oocytes collected from the experimental group of non-pregnant adult ewes was higher ( p ≤ 0.007) than those collected from the group of pregnant adult ewes (2.67 ± 0.19 vs. 2.18 ± 0.15 oocytes/group, respectively). The blastocyst rate was higher ( p ≤ 0.0001) in the non-pregnant adult group (36.39%) than in the young (17.96%). The ratio of demi-embryos that recovered the blastocoelic cavity was higher ( p < 0.05) in the young group (81.25%) than in the pregnant adult group (59.38%). The diameter of the demi-embryos was higher ( p < 0.05) in the non-pregnant adult group (186.54 ± 8.70 μm) than those in the young and pregnant adult groups. Conclusions In conclusion, the in vitro embryo production efficiency was highest when using oocytes from non-pregnant adult ewes under the conditions of this study.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In vitro embryo production from ewes at different physiological stages

et al. 2023

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“…Doudna, Charpentier, and co-workers [Jinek et al, 2012;Doudna and Charpentier, 2014] focused on and exposed how the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) protein complexes (CRISPR/Cas), which roughly 50% of bacteria and 90% of archaea possess [Makarova et al, 2015], act together to excise and/or neutralize intrusive foreign genetic material by precision genome-editing and manipulation [Diemen et al, 2015;Nelles et al, 2016;Nun~ez et al, 2016;Wright et al, 2016]. The CRISPR/Cas9 system, and evolving engineered variations [Dunn and Pinkert, 2014;Abudayyeh et al, 2016;Komor et al, 2016;Luo et al, 2016;Slaymaker et al, 2016;Wang et al, 2016a,b], are quickly becoming the gene-editing tools of choice for specificity in human cells-with great potential for drug discovery and development [Golkar et al, 2016], and for correcting disease-causing mutatedgenes in human embryos [Boeke et al, 2016;Ledford, 2016], but hopefully not as an incremental step toward the creation of "designer" babies [Godke et al, 2014;Pinkert et al, 2014;Bosley et al, 2015;Annas, 2016;Greely, 2016].…”

Section: Synthetic Biology Potentialmentioning

confidence: 99%

Epigenetic Treatment of Persistent Viral Infections

Moos¹,

Pinkert

Irwin

et al. 2016

Drug Development Research

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Preclinical Research Approximately 2,500 years ago, Hippocrates used the word herpes as a medical term to describe lesions that appeared to creep or crawl on the skin, advocating heat as a possible treatment. During the last 50 years, pharmaceutical research has made great strides, and therapeutic options have expanded to include small molecule antiviral agents, protease inhibitors, preventive vaccines for a handful of the papillomaviruses, and even cures for hepatitis C virus infections. However, effective treatments for persistent and recurrent viral infections, particularly the highly prevalent herpesviruses, continue to represent a significant unmet medical need, affecting the majority of the world's population. Exploring the population diversity of the human microbiome and the effects its compositional variances have on the immune system, health, and disease are the subjects of intense investigational research and study. Among the collection of viruses, bacteria, fungi, and single-cell eukaryotes that comprise the human microbiome, the virome has been grossly understudied relative to the influence it exerts on human pathophysiology, much as mitochondria have until recently failed to receive the attention they deserve, given their critical biomedical importance. Fortunately, cellular epigenetic machinery offers a wealth of druggable targets for therapeutic intervention in numerous disease indications, including those outlined above. With advances in synthetic biology, engineering our body's commensal microorganisms to seek out and destroy pathogenic species is clearly on the horizon. This is especially the case given recent breakthroughs in genetic manipulation with tools such as the CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated) gene-editing platforms. Tying these concepts together with our previous work on the microbiome and neurodegenerative and neuropsychiatric diseases, we suggest that, because mammalian cells respond to a viral infection by triggering a cascade of antiviral innate immune responses governed substantially by the cell's mitochondria, small molecule carnitinoids represent a new class of therapeutics with potential widespread utility against many infectious insults. Drug Dev Res 78 : 24-36, 2017. © 2016 Wiley Periodicals, Inc.

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“…This tool allows a genetically superior female to produce a greater number of embryos (and consequently offspring) during her reproductive life. Under natural mating or artificial insemination systems, a cow would give birth to only one offspring per year [3].…”

Section: Introductionmentioning

confidence: 99%

Effect of cryopreservation of in vitro produced embryos on pregnancy rate of cows transferred at fixed time in the dry tropics

Kayser-Alarcón,

Montiel-Palacios,

Canseco-Sedano

et al. 2024

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The objective of this study was to determine the effect of cryopreservation (CP) of in vitro produced embryos (EIV) on the pregnancy rate (PR) of cows transferred at fixed time (FTET) in the dry tropics. The experimental design was completely randomized with a 2×2 factorial arrangement. The sample consisted of 280 embryos produced in vitro. The factors were the CP (vitrified and fresh) and the Rural Development District (DDR 01 and 02). The experiment consisted of 70 replicates per treatment: T1) fresh transferred embryos; T2) vitrified embryos; T3) DDR + fresh embryos; and 4) DDR + vitrified embryos. The pregnancy diagnosis was carried out 60 days after embryo transfer. The Xi2 test was used to analyze PR which was the response variable per treatment. PR was higher in fresh-transferred embryos than in vitrified embryos (53.6±4.2 vs 27.1±3.7; P<0.05), and in DDR02 than in DDR01 (47.1±4.2 vs 33.6±3.9; P<0.05). Therefore, the transfer of fresh embryos and the DDR02 had a positive overall effect on PR (40.3%).

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Assisted Reproductive Technologies and Embryo Culture Methods for Farm Animals

Cited by 9 publications

References 259 publications

In vitro embryo production from ewes at different physiological stages

In vitro embryo production from ewes at different physiological stages

Epigenetic Treatment of Persistent Viral Infections

Effect of cryopreservation of in vitro produced embryos on pregnancy rate of cows transferred at fixed time in the dry tropics

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