<i>In vitro</i> embryo production from ewes at different physiological stages

Lorenzo-Torres, Alfredo; Rangel-Santos, Raymundo; Ruíz-Flores, Agustín; Ambríz-García, Demetrio Alonso

doi:10.4142/jvs.22168

Cited by 4 publications

(1 citation statement)

References 37 publications

(56 reference statements)

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“…Hernández et al [47] reported a low number of nuclei (44.2 ± 9.9) present in parthenogenetic blastocysts of O. aries and in interspecies cloned blastocysts of O. aries and Ovis canadensis mexicana (46.7 ± 8.1 nuclei). Lorenzo-Torres et al [48] reported 122 nuclei in good-quality blastocysts produced by IVF. These results are lower than those obtained in the present study, but they are similar to those reported by Agata et al [49], Mastrorocco et al [50], and Nadri et al [51].…”

Section: Effect Of Resveratrol On Embryo Qualitymentioning

confidence: 99%

Resveratrol Reduces ROS by Increasing GSH in Vitrified Sheep Embryos

González-Garzón,

Ramón-Ugalde,

Ambríz-García

et al. 2023

Animals

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The in vitro production and cryopreservation of mammalian embryos generates reactive oxygen species (ROS) due to conditions of the system that can overcome their antioxidant protection. Resveratrol is an antioxidant used in in vitro systems to improve blastocyst rates, but its effect on antioxidant enzymes such as glutathione (GSH) in embryos produced by in vitro fertilization (IVF) after vitrification has not been reported. The objective of this study was to evaluate the effects of resveratrol on the in vitro maturation medium (IVM) of sheep oocytes (Ovis aries) on the levels of ROS and GSH in embryos produced by IVF subjected to vitrification. Resveratrol was added at 0 µM, 0.25 µM, 0.5 µM, and 1 µM during oocyte in vitro maturation (IVM). Matured oocytes were fertilized with thawed ram sperm. Embryos were cultured in sequential media until blastocysts, were then vitrified for 24 h, and, after heating, they were stained with DCFH-DA (2′,7′-dichlorodihydrofluorescein diacetate) to determine the presence of ROS and with Cell Tracker Blue® for the presence of GSH. The quantitative values of ROS and GSH were obtained through the Image J image processor. The results showed that resveratrol increased GSH and decreased ROS production (p < 0.05) in a dose-dependent manner. It is concluded that its use in sheep oocytes during IVM has a beneficial effect on embryos produced by IVF subjected to vitrification by decreasing reactive oxygen species levels and facilitating the generation of embryo antioxidant enzymes like glutathione.

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Section: Effect Of Resveratrol On Embryo Qualitymentioning

confidence: 99%

Resveratrol Reduces ROS by Increasing GSH in Vitrified Sheep Embryos

González-Garzón,

Ramón-Ugalde,

Ambríz-García

et al. 2023

Animals

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A retrospective analysis of sheep generated by somatic cell nuclear transfer

Liu,

Stott,

Regouski

et al. 2024

Theriogenology

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In vitro embryo production in small ruminants: what is still missing?

Souza-Fabjan,

Leal,

Monteiro

et al. 2023

Anim. Reprod.

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In vitro embryo production (IVEP) is an extremely important tool for genetic improvement in livestock and it is the biotechnology that has grown the most recently. However, multiple ovulation followed by embryo transfer is still considered the leading biotechnology for embryo production in small ruminants. This review aimed to identify what is still missing for more efficient diffusion of IVEP in small ruminants, going through the IVEP steps and highlighting the main factors affecting the outcomes. Oocyte quality is essential for the success of IVEP and an aspect to be considered in small ruminants is their reproductive seasonality and strategies to mitigate the effect of season. The logistics for oocyte collection from live females is more complex than in cattle, and tools to simplify this collection system and/or to promote an alternative way of recovering oocytes may be an important point in this scenario. The heterogeneity of oocytes collected from growing follicles in live females or from ovaries collected from abattoirs remains a challenge, and there is a demand to standardize/homogenize the hormonal stimulatory protocols and IVM protocols for each source of oocytes. The use of sexed semen is technically possible, however the low market demand associated with the high costs of the sexing process prevents the routine use of this technique, but its higher availability is an important aspect aiming for greater dissemination of IVEP. New noninvasive approaches for embryo selection are key factors since the selection for transfer or cryopreservation is another difficulty faced among laboratories. Embryo selection is based on morphological traits, although these are not necessarily reliable in predicting pregnancy. Several issues described in this review must be considered by researchers in other to promote the diffusion of IVEP in small ruminants.

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In vitro embryo production from ewes at different physiological stages

Cited by 4 publications

References 37 publications

Resveratrol Reduces ROS by Increasing GSH in Vitrified Sheep Embryos

Resveratrol Reduces ROS by Increasing GSH in Vitrified Sheep Embryos

A retrospective analysis of sheep generated by somatic cell nuclear transfer

In vitro embryo production in small ruminants: what is still missing?

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