Assisted reproduction in Nile tilapia Oreochromis niloticus: Milt preservation, spawning induction and artificial fertilization

Use of hCG hormone in the natural and artificial reproduction of Nile tilapia ( Oreochromis niloticus )

“…The method of hCG administration for artificial reproduction is considered inconsistent among different studies and authors (Piamsomboon et al, 2019). Since the inconsistencies can be related to the use of different protocols, in the second experiment, the effects of different doses of hCG, administration routes, the number of injections and the interaction of these factors on reproductive variables were jointly studied.…”

“…Synchronization of ovulation or spawning by hormonal protocols can allow better reproductive management in a large diversity of domestic animals (Martemucci & D’Alessandro, 2011; Piamsomboon et al, 2019; Srisakultiew & Wee, 1988). In a wide range of marine and freshwater fish species, exogenous hormones have been successfully used to induce ovulation and spermiation (Mylonas et al, 2017; Zadmajid, 2016; Zadmajid et al, 2018; Zohar & Mylonas, 2001).…”

“…In a wide range of marine and freshwater fish species, exogenous hormones have been successfully used to induce ovulation and spermiation (Mylonas et al, 2017; Zadmajid, 2016; Zadmajid et al, 2018; Zohar & Mylonas, 2001). These protocols are species‐specific; however, they aim at common goals including the manipulation of follicle development, the improvement of oocyte quality and the increase in egg/embryo quantity and embryo survival (Bisinotto et al, 2014; Martemucci & D’Alessandro, 2011; Mylonas et al, 2010; Piamsomboon et al, 2019; Saldarriaga et al, 2007). Reproductive synchronization protocols have been applied to optimize both natural and artificial reproduction in fish (Mylonas et al, 2010; Srisakultiew & Wee, 1988).…”

“…Nile tilapia ( Oreochromis niloticus ), one of the most extensively cultured tropical freshwater species (FAO, 2018), is a multiple spawner and maternal mouthbrooder that easily reproduces under captivity conditions (Bhujel, 2000; Piamsomboon et al, 2019; Pullin et al, 1991). Despite this, the seed production of Nile tilapias faces limitations, such as the low egg production and unpredictable spawning (Bhujel, 2000; Little et al, 1993; Ng & Romano, 2013).…”

“…Protocols of spawning induction by hormones in Nile tilapia have been applied mainly for collecting of gametes in procedures of chromosome manipulations (Alvarenga et al, 2020; Fernandes et al, 2013; Pradeep et al, 2014), for reproductive biology studies (Alvarenga et al, 2017; Babikeran & Ibrahim, 1979; El‐Gamal & El‐Greisy, 2005) and for facilitating the breeding of Nile tilapia in pair mating selection (Fernandes et al, 2013; Piamsomboon et al, 2019). However, according to our knowledge, there is only one study of hormonal protocols for synchronization of natural spawning described in Nile tilapia (Srisakultiew & Wee, 1988), in which a partial success of synchronization of spawning was obtained with 0.25 and 0.5 IU hCG/g of female.…”