Assisted fertilization with intracytoplasmic sperm injection

Tsirigotis, M.; Yang, Dunsong; Redgment, Christopher; Nicholson, Natasha; Pelekanos, M.; Craft, Ian

doi:10.1016/s0015-0282(16)57004-6

Cited by 35 publications

(7 citation statements)

References 12 publications

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“…In our large cohort, we confirm the superiority of ICSI over IVF in terms of strong ART outcomes as previously demonstrated. Indeed, several uncontrolled studies suggested that the pregnancy rate ranged from 9% to 12% in IVF 57 and from 16% to 26% in ICSI cycles 58–60 . However, the real reason for this difference is not clear.…”

Section: Discussionmentioning

confidence: 99%

“…Indeed, several uncontrolled studies suggested that the pregnancy rate ranged from 9% to 12% in IVF 57 and from 16% to 26% in ICSI cycles. 58 , 59 , 60 However, the real reason for this difference is not clear. Potentially, ICSI cycles were initially applied to couples with male infertility causes, in which normal female fertility could lead to better quality embryos with superior implantation rates.…”

Section: Discussionmentioning

confidence: 99%

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Are sperm parameters able to predict the success of assisted reproductive technology? A retrospective analysis of over 22,000 assisted reproductive technology cycles

et al. 2021

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Background: An explosive increase in couples attending assisted reproductive technology has been recently observed, despite an overall success rate of about 20%-30%.Considering the assisted reproductive technology-related economic and psycho-social costs, the improvement of these percentages is extremely relevant. However, in the identification of predictive markers of assisted reproductive technology success, male parameters are largely underestimated so far. Study design: Retrospective, observational study.Objectives: To evaluate whether conventional semen parameters could predict assisted reproductive technology success. Materials and methods:All couples attending a single third-level fertility center from 1992 to 2020 were retrospectively enrolled, collecting all semen and assisted reproductive technology parameters of fresh cycles. Fertilization rate was the primary end-point, representing a parameter immediately dependent on male contribution. Pregnancy and live birth rates were considered in relation to semen variables. Statistical analyses were performed using the parameters obtained according to the World Health Organization manual editions used for semen analysis.Results: Note that, 22,013 in vitro fertilization and intracytoplasmic sperm injection cycles were considered. Overall, fertilization rate was significantly lower in patients with abnormal semen parameters compared to normozoospermic men, irrespective of the World Health Organization manual edition. In the in vitro fertilization setting, both progressive motility (p = 0.012) and motility after capacitation (p = 0.002) significantly predicted the fertilization rate (statistical accuracy = 71.1%). Sperm motilities also predicted pregnancy (p < 0.001) and live birth (p = 0.001) rates. In intracytoplasmic sperm injection cycles, sperm morphology predicted fertilization rate (p = 0.001, statistical accuracy = 90.3%). Sperm morphology significantly predicted bothThis is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Are sperm parameters able to predict the success of assisted reproductive technology? A retrospective analysis of over 22,000 assisted reproductive technology cycles

et al. 2021

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“…partial zonal dissection and subzonal insemination, was expected to improve fertilization and pregnancy rates, but the results were disappointing [15,16]. However, with the establishment of the clinical application of the IeSI technique, and the high fertilization and pregnancy rates observed in cases with extreme male-factor infertility [2,4] it was inevitable that sperm obtained by microsurgical methods from sites proximal to an obstruction would also be used for IeSI to try to achieve a high incidence of fertilization and pregnancy. Factors which may influence the success of IVF following MESA, e.g.…”

Section: Methodsmentioning

confidence: 99%

“…The management of male-factor infertility has undergone dramatic changes in the last decade. The introduction of assisted fertilization and particularly intracytoplasmic sperm injection (ICSI) [1][2][3][4] have made treatment possible for those who were previously considered sterile, and has invalidated the concept that a sperm should go through the full length of the genital tract before it is able to achieve fertilization [5].…”

Section: Introductionmentioning

confidence: 99%

Simplified sperm retrieval and intracytoplasmic sperm injection in patients with azoospermia

Tsirigotis¹,

Pelekanos²,

Yazdani³

et al. 1995

British Journal of Urology

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“…One technique of assisted fertilisation, intracytoplasmic sperm injection (ICSI), might circumvent the second of these 2 reasons for the poor results of IVF in equids. The technique of ICSI is now used widely in human assisted fertility clinics (Tsirigotis et al 1994;Palermo et al 1996). There have been several reports of the use of ICSI in farm animals, including the production of offspring in cattle (Goto et al 1990), a confirmed pregnancy in the horse (Squires et al 1996) and cleavage of horse oocytes (Meintjes et al 1996).…”

Section: Introduction Oocyte Collection and Maturationmentioning

confidence: 99%

Treatment of equine oocytes with A23187 after intracytoplasmic sperm injection

Kato

Seidel

Squires

et al. 1997

Equine Veterinary Journal

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Summary In vitro matured horse oocytes with a first polar body (n = 68) were each injected with a single spermatozoon and divided into 2 groups: Group 1 oocytes were treated with 10 μM calcium ionophore A23187 for 5 min while Group 2 oocytes received no activation treatment. After culture in vitro for 2 days, significantly more oocytes treated with A23187 (5/24, 21%) cleaved than oocytes without activation treatment (2/44, 5%, P < 0.05). All 7 cleaved zygotes from both treatment groups were transferred to recipient mares but no pregnancies resulted.

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Assisted fertilization with intracytoplasmic sperm injection

Cited by 35 publications

References 12 publications

Are sperm parameters able to predict the success of assisted reproductive technology? A retrospective analysis of over 22,000 assisted reproductive technology cycles

Are sperm parameters able to predict the success of assisted reproductive technology? A retrospective analysis of over 22,000 assisted reproductive technology cycles

Simplified sperm retrieval and intracytoplasmic sperm injection in patients with azoospermia

Treatment of equine oocytes with A23187 after intracytoplasmic sperm injection

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