Assisted fertilization: Micro-assisted fertilization with human gametes

Fishel, Simon; Dowell, Ken; Timson, Judy; Green, Steve; Hall, Jenny; Klentzeris, Lucas D.

doi:10.1093/oxfordjournals.humrep.a137933

Cited by 21 publications

(4 citation statements)

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“…Thus, proper management of these patients is critical to maximizing fertility outcomes and usage of limited resources; yet, controversy about management of these patients remains. This confusion has been driven by continuous modifications of the morphologic scoring criteria (1, 3-8), advancement of potential therapeutic techniques (14,15), and advancement of IVF protocols and reagents (30,31). After considering these factors, we were curious how to make current clinical recommendations for management of teratozoospermic patients when they initiate IVF.…”

Section: Discussionmentioning

confidence: 99%

“…Laboratory techniques such as high insemination concentrations of sperm (14) and intracytoplasmic sperm injection (ICSI) (15) were developed to treat various causes of poor fertilization in IVF; thus, they were investigated as potential treatments for teratozoospermia. Several studies validated the use of these procedures by showing an improvement in fertilization rate and a decrease in fertilization failure rate (16)(17)(18).…”

mentioning

confidence: 99%

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Isolated teratozoospermia does not affect in vitro fertilization outcome and is not an indication for intracytoplasmic sperm injection

Keegan

Barton

Sanchez

et al. 2007

Fertility and Sterility

101

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Isolated teratozoospermia does not affect in vitro fertilization outcome and is not an indication for intracytoplasmic sperm injection

Keegan

Barton

Sanchez

et al. 2007

Fertility and Sterility

101

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“…When enough spermatozoa were present in the semen sample, sperm morphology was evaluated according to Tygerberg's strict criteria (Kruger et al, 1986). We adopted the unit 'total number of motile spermatozoa in the ejaculate' (TMSE), as recommended by Fishel et al (1993), to classify the semen samples.…”

Section: Patient Selection and Semen Evaluationmentioning

confidence: 99%

Two essential steps for a successful intracytoplasmic sperm injection: injection of immobilized spermatozoa after rupture of the oolema

Vanderzwalmen¹,

Bertin²,

Lejeune³

et al. 1996

Human Reproduction

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A total of 740 cycles of intracytoplasmic sperm injection (ICSI) were performed: 625 cycles when < 6 x 10(5) total motile spermatozoa were harvestable from the ejaculate and 115 cycles in cases with a history of previous fertilization failure after classic in-vitro fertilization or subzonal sperm injection. An average of two pronuclei were observed in 63% of the injected oocytes, allowing 725 transfers of a maximum of three embryos (98%). Of 214 pregnancies initiated, 179 were established (25% of ICSI attempts). Because the fertilization rates from our initial 80 ICSI cycles were 2-fold less than those achieved previously, we changed the injection procedure and analysed, in 740 ICSI attempts, the importance of interfering technical factors and how to establish a successful ICSI programme. A remarkable change in the fertilization rate up to 68% (595 cycles) occurred when two steps in the injection procedure were performed well, i.e. immobilization of the spermatozoon and placement of the spermatozoon into the ooplasm after cytoplasmic aspiration into the pipette until oolema rupture. This immobilization, by touching the tail with the pipette, is mandatory for increasing the percentage of fertilization, even with totally non-motile spermatozoa (41%). Because aspiration of the cytoplasm is an invasive part of the ICSI procedure and influences the quality of the embryos, it is essential to reduce the amount of cytoplasm drawn into the pipette. This could be attained by using a spikeless injection pipette with the smallest possible internal diameter.

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“…6 The third method, subzonal sperm insertion (SZI) in which sperm totally bypass interaction with the zona pellucida and are directly placed in the perivitelline space has gained widespread clinical acceptance. [7][8][9] The fourth technique, intracytoplasmic injection (ICSI) has been successfully developed and applied in Belgium; it involves placement of a single sperm in the ooplasm bypassing both zona and oolemma interaction. 10,11 Normal healthy babies have been born from PZD, SZI, and ICSI.…”

Section: Micromanipulationmentioning

confidence: 99%

Efficiency Enhancement of Manipulated Male and Female Gametes

Minhas¹

1994

Semin Reprod Med

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Assisted fertilization: Micro-assisted fertilization with human gametes

Cited by 21 publications

References 0 publications

Isolated teratozoospermia does not affect in vitro fertilization outcome and is not an indication for intracytoplasmic sperm injection

Isolated teratozoospermia does not affect in vitro fertilization outcome and is not an indication for intracytoplasmic sperm injection

Two essential steps for a successful intracytoplasmic sperm injection: injection of immobilized spermatozoa after rupture of the oolema

Efficiency Enhancement of Manipulated Male and Female Gametes

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