Assisted delivery of anti-tumour platinum drugs using DNA-coiling gold nanoparticles bearing lumophores and intercalators: towards a new generation of multimodal nanocarriers with enhanced action

Caballero, Ana B.; Cardo, Lucia; Claire, Sunil; Craig, James S.; Hodges, Nikolas J.; Vladyka, Anton; Albrecht, Tim; Rochford, Luke A.; Pikramenou, Zoe; Hannon, Michael J.

doi:10.1039/c9sc02640a

Cited by 20 publications

(15 citation statements)

References 72 publications

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“…Gold nanoparticles are known for their excellent biocompatibility with biomolecules and possess remarkable structural, electronic, magnetic, optical and catalytic properties [26]. Caballero et al [27] found that α-lipoic acid/aminoanthraquinone functionalized gold nanoparticles caused notable coiling/wrapping of DNA despite their anionic surface charge. The functionalization of magnetic nanoparticles with gold was demonstrated through the conjugation of thiolated DNA to the gold shell [28].…”

Section: Resultsmentioning

confidence: 99%

constaNt current chronopotenTiometry Study of dna for the Detection of African Swine Fever Virus

Rychly¹,

Hosnedlová

Parikesit

et al. 2021

NANOCON 2021 Conference Proeedings

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African Swine Fever Virus (ASFV) is a DNA virus of the Asfivirus genus of the Asfarviridae family that is found in blood, body fluids, and internal organs. ASFV was described more than 40 years ago. This virus spreads pandemically and the mortality rate of the virus-related disease ranges from 90 to 100 %. The aim of this study was to propose the detection of the specific nucleic acid of ASFV using electrochemical detection. Determination of DNA was conducted by chronopotentiometric stripping analysis (time of accumulation 120 s, stripping current 20, 10, 5, 1 µA, measurement time 240 s). The volume of the analyzed sample was 10 µL. Sufficient analysis duration (200-600 s) is required to fully record the H peak signal of DNA at a given applied current. Extending the DNA analysis time at a given current significantly improved the dt/dE records of the H peak and the reproducibility of the analysis (RSD up to 10%). It was found that the suitable time at which the H peak is plotted reliably is around 240 s. The detected dsDNA concentration dependence (0 to 60 µg/mL) on the catalytic signal of the H peak was described according to the equation y = 260.4 + 366.7x, r = 0.961, RSD 23.1%. Subsequently, the SPION particle was modified with dsDNA. After the thermal release of DNA from SPION, DNA was immediately determined by chronopotentiometric stripping analysis method.

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Section: Resultsmentioning

confidence: 99%

constaNt current chronopotenTiometry Study of dna for the Detection of African Swine Fever Virus

Rychly¹,

Hosnedlová

Parikesit

et al. 2021

NANOCON 2021 Conference Proeedings

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“…In order to achieve conjugation with nanocarriers the structure of crucial clinical platinum drugs such as cisplatin (a) oxaliplatin, and (b) carboplatin (c) (Figure 1) has to be modified with suitable functionalized linkers [26,27]. This concept is based on covalent binding of the drug to relevant nanocarrier.…”

Section: Platinum Drugs Conjugatesmentioning

confidence: 99%

Nanoarchitectonics: Complexes and Conjugates of Platinum Drugs with Silicon Containing Nanocarriers. An Overview

Piorecka

Kurjata

Stańczyk

2021

IJMS

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The development in the area of novel anticancer prodrugs (conjugates and complexes) has attracted growing attention from many research groups. The dangerous side effects of currently used anticancer drugs, including cisplatin and other platinum based drugs, as well their systemic toxicity is a driving force for intensive search and presents a safer way in delivery platform of active molecules. Silicon based nanocarriers play an important role in achieving the goal of synthesis of the more effective prodrugs. It is worth to underline that silicon based platform including silica and silsesquioxane nanocarriers offers higher stability, biocompatibility of such the materials and pro-longed release of active platinum drugs. Silicon nanomaterials themselves are well-known for improving drug delivery, being themselves non-toxic, and versatile, and tailored surface chemistry. This review summarizes the current state-of-the-art within constructs of silicon-containing nano-carriers conjugated and complexed with platinum based drugs. Contrary to a number of other reviews, it stresses the role of nano-chemistry as a primary tool in the development of novel prodrugs.

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“…Luminescent nanoparticles can be monitored in live cells using conventional microscopy techniques, enabling time-resolved information to be collected to track AuNP uptake, fate, and drug delivery. 5 We have previously used luminescent metals to decorate the surface of gold nanoparticles to produce luminescent nanoparticles with a large Stokes shift and high photostability 6 − 8 and show cellular internalization in the absence of cytotoxicity. 9 We have studied the design of the metal complexes for their distance from the gold particle to eliminate any quenching mechanisms from the gold.…”

Section: Introductionmentioning

confidence: 99%

Quantification by Luminescence Tracking of Red Emissive Gold Nanoparticles in Cells

Dosumu

Claire

Watson

et al. 2021

JACS Au

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Optical microscopy techniques are ideal for live cell imaging for real-time nanoparticle tracking of nanoparticle localization. However, the quantification of nanoparticle uptake is usually evaluated by analytical methods that require cell isolation. Luminescent labeling of gold nanoparticles with transition metal probes yields particles with attractive photophysical properties, enabling cellular tracking using confocal and time-resolved microscopies. In the current study, gold nanoparticles coated with a red-luminescent ruthenium transition metal complex are used to quantify and track particle uptake and localization. Analysis of the red-luminescence signal from particles is used as a metric of cellular uptake, which correlates to total cellular gold and ruthenium content, independently measured and correlated by inductively coupled plasma mass spectrometry. Tracking of the luminescence signal provides evidence of direct diffusion of the nanoparticles across the cytoplasmic membrane with particles observed in the cytoplasm and mitochondria as nonclustered “free” nanoparticles. Electron microscopy and inhibition studies identified macropinocytosis of clusters of particles into endosomes as the major mechanism of uptake. Nanoparticles were tracked inside GFP-tagged cells by following the red-luminescence signal of the ruthenium complex. Tracking of the particles demonstrates their initial location in early endosomes and, later, in lysosomes and autophagosomes. Colocalization was quantified by calculating the Pearson’s correlation coefficient between red and green luminescence signals and confirmed by electron microscopy. Accumulation of particles in autophagosomes correlated with biochemical evidence of active autophagy, but there was no evidence of detachment of the luminescent label or breakup of the gold core. Instead, accumulation of particles in autophagosomes caused organelle swelling, breakdown of the surrounding membranes, and endosomal release of the nanoparticles into the cytoplasm. The phenomenon of endosomal release has important consequences for the toxicity, cellular targeting, and therapeutic future applications of gold nanoparticles.

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Assisted delivery of anti-tumour platinum drugs using DNA-coiling gold nanoparticles bearing lumophores and intercalators: towards a new generation of multimodal nanocarriers with enhanced action

Abstract: Nanocarriers with unusual DNA binding properties provide enhanced cytotoxic activity beyond that conferred by the platinum agents they release.

Cited by 20 publications

References 72 publications

constaNt current chronopotenTiometry Study of dna for the Detection of African Swine Fever Virus

constaNt current chronopotenTiometry Study of dna for the Detection of African Swine Fever Virus

Nanoarchitectonics: Complexes and Conjugates of Platinum Drugs with Silicon Containing Nanocarriers. An Overview

Quantification by Luminescence Tracking of Red Emissive Gold Nanoparticles in Cells

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