Assignment of the human dihydrofolate reductase gene to the q11----q22 region of chromosome 5.

Abstract

Cells from a dihydrofolate reductase-deficient Chinese hamster ovary cell line were hybridized to human fetal skin fibroblast cells. Nineteen dihydrofolate reductase-positive hybrid clones were isolated and characterized. Cytogenetic and biochemical analyses of these clones have shown that the human dihydrofolate reductase (DHFR) gene is located on chromosome 5. Three of these hybrid cell lines contained different terminal deletions of chromosome 5. An analysis of the breakpoints of these deletions has demonstrated that the DHFR gene resides in the q11----q22 region.

Report of the committee on the genetic constitution of chromosome 5

“…Locus Keats et al, 1991Wciffenbach et al, 1991Gilliam et al, 1989Feder et al, 1987Maurer et al, 1985Funanage et al, 1984Anagnou et al, 1984Yang et al, 1984Chcn et al, 1984Maurer et al, 1984bMaurer et al, 1984aWolman et al, 1983Masters and Attardi, 1983 Sherrington et al, 1991Gilliam et al, 1989St. Clair et al, 1989Kennedy et al, 1988Leppert et al, 1987bC Daniels et al, 1991Mclki et al, 1990bMclki et al, 1990aGilliam ct al., 1990Brzustowicz et al, 1990C Nomiyama et al, 1986Carlock et al, 1985Carlock and Wasmuth, 1985Su et al, 1984 5ql Lentes et al, 1988Frielle et al, 1987Emorine et al, 1987Kobilka et al, 1987bSchofield et al, 1987Kobilka et al, 1987aDixon et al, 1986Sheppard et al, 1983 5q31-q32 GRL + glucocorticoid receptor A,R,S c Francke and Foellmer, 1989 5q31-q33 DHLAG major histocompatibility complex, class II, gamma polypeptide A,R,S Theriault et al, 1989Evans, 1988Giuffra et al, 1988Hollenberg et al, 1985Weinberger et al, 1985Eliard et al, 1985Gehring et al, 1985…”

No Evidence for Linkage between Chromosome 5 Markers and Schizophrenia

“…The four allele system asso ciated with 105-599HA and the two alleles seen with 105-153RA have been reported elsewhere [3]. We estimated the distance between 105-153 RA and CHB213 to be 0.144 M. CHB213, a dihydrofolate reduc- tase gene (DHFR) probe [14], had frag ment sizes similar to those previously re ported [15]. The listed distances refer to male recombination fractions.…”

Cystic fibrosis and Helicobacter pylori gastritis, megaloblastic anaemia, subnormal mentality and minor anomalies in two siblings: a new syndrome?

“…It is possible that the deceased father had the autosomal dominant DHFR transmitted to his children; in addition he and his wife were heterozygotes for CF. A contiguous gene defect involving both the CF and the DHFR is ruled out since CF is located on the long arm of chromosome 7 [4] and the DHFR gene is assigned to the long arm of chromosome 5 [1]. Another alternative is that these sibs have a distinct autosomal recessive disorder resembling CF and causing folate deficiency through an enzyme defect.…”