Assessment the Activity of Some Enzymes and Antibiotic Substances Sensitivity on Pathogenic Bacteria Species

Abstract

Microbial and opportunistic pathogens cause serious problem to human host by releasing different compounds which are involved in colonization, invasion or in alteration of immune process. In this study we propose ourselves to analyze the virulence profile of microorganisms involved in urinary tract infections and bacterial vaginosis in order to compare the virulence mechanisms developed by these microorganisms. We assessed the virulence profile (motility, adhesion to HeLa cell line, biofilm formation, production of enzymes, antibiotic susceptibility) using phenotypic methods. The microbial strains isolated from urinary tract infections present a high ability to release amylases, caseinase, siderophore-like, half of them have present high motility and also were highly resistant to antibiotics, while the microbial strains isolated from bacterial vaginosis present a high ability to bind human epithelial cells and to release hemolyzin and DN-ase.

Bioavailability and Bioactivities of Polyphenols Eco Extracts from Coffee Grounds after In Vitro Digestion

“…For this study, two Gram-positive strains (S. aureus ATCC BAA 1026, S. aureus 1004, MRSA phenotype, and two Gram-negative strains ( E. coli ACTCC 25922; E. coli B11, ESBL phenotype) were selected. The multidrug-resistant (MDR) strains were isolated from wound infections and were previously characterized [ 28 , 29 ]. Ethanol 50% was used as control.…”

The Evolution of Measles in Romania Between 2016 and 2018. A comprehesive overview

“…In the age group 0-6 months such infections are predominantly caused by Candida albicans species and can affect the oropharynx and/or the esophagus of persons with dysfunctions of the adaptive immune system [21][22][23]. Because simple changes in the physiology of the human organism can lead to disturbance of the balance in microbiota, the Candida genus species exhibit a very high pathogen profile [24][25][26].…”

Synthesis and Structural Characterization of Some 1,3-Dithiol-2-ylium Salts

“…Amongst these, sulfur and nitrogen-containing heterocycles receive a great deal of attention [6][7][8][9][10][11][12][13][14][15][16]. In the field of conducting materials charge-transfer [17][18][19][20][21][22] and push-pull [23][24][25][26][27][28][29][30][31][32][33][34][35][36][37][38] compounds have important applications. 1,3-Dithiolium derivatives have been found to exhibit an excellent biological activity, especially against gram-positive and gram-negative bacteria [39].…”