Assessment of Yam mild mosaic virus coat protein gene sequence diversity reveals the prevalence of cosmopolitan and African group of isolates in Ghana and Nigeria

Abstract

Highlights Sequence diversity show lack of geographical association between isolates from Ghana and Nigeria. YMMV isolates from Ghana and Nigeria fall within four of the 11 monophyletic groups. Need for stringent control of germplasm exchange as infection is mainly by infected tubers.

Viruses of Yams (Dioscorea spp.): Current Gaps in Knowledge and Future Research Directions to Improve Disease Management

“…RPA was shown to have the same level of sensitivity as conventional PCR in YMV detection [41], whereas LAMP was around 100 times more sensitive than conventional PCR [48]. A drawback of the LAMP assay is that it requires a set of six primers, which can be challenging to design, especially when dealing with genetically highly variable viruses such as YMMV and yam badnaviruses [26,46,48,63,83]. Despite the advantages of RPA and LAMP techniques in virus diagnostics, these are recently developed techniques with only a few reports on their use for the diagnosis of yam potyviruses to date.…”

“…These assays have also been reported to be more tolerant than PCR to inhibitory substances present in yam nucleic acid samples [42,43]. RPA was shown to have the same level of sensitivity as conventional PCR in YMV detection [41], whereas LAMP was around 100 times more sensitive than conventional PCR [48]. A drawback of the LAMP assay is that it requires a set of six primers, which can be challenging to design, especially when dealing with genetically highly variable viruses such as YMMV and yam badnaviruses [26,46,48,63,83].…”

“…However, the role of recombination in the evolutionary process of yam viruses remains poorly documented overall and should be investigated further. Overall, phylogeography approaches have proved an efficient method for unravelling the complex processes driving the emergence, spread, and evolution of yam viruses [21,39,[46][47][48]. It would be advisable to extend these studies to all viruses infecting yams.…”

“…Major efforts have been, and are still, placed on the development of accurate, sensitive, and cost-effective tools for the diagnosis of yam viruses [8,38,[41][42][43]58,61]. However, such development can be particularly challenging because most yam viruses display a high molecular variability that can affect the accuracy and sensitivity of serological and molecular diagnoses [40,48,62,63].…”

Editorial Special Issue: Plant interactions with microbes and environment

“…Nkere et al [ 5 ] describe the prevalence of Yam mild mosaic virus (YMMV), a potyvirus in Ghana and Nigeria by assessing the coat protein gene sequence diversity. Yam ( Dioscorea spp .…”