Assessment of various stresses and carbohydrates for their effect on the induction of embryogenesis in isolated wheat microspores

Indrianto, Ari; Heberle‐Bors, Erwin; Touraev, Alisher

doi:10.1016/s0168-9452(99)00022-9

Cited by 64 publications

(43 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Isolated Orchid pollinia of Dendrobium hybrid 1, Vanda tricolor and Spathoglottis plicata were subjected to starvation in carbohydrate and nitrogen-free medium B, which was shown to trigger the formation of embryogenic microspores in wheat and tobacco (Indrianto, et al, 1999;Touraev et al, 1997). Pollinia were cultured in starvation medium B and incubated at various temperatures and duration of time to evaluate embryogenic response and simultaneously the block of gametophytic pathway.…”

Section: Starvation Of Isolated Orchid Polliniamentioning

confidence: 99%

“…Sucrose starvation, in particular, appears to be the major signal controlling the developmental fate of tobacco microspores, as it is the most effective treatment for embryogenic induction from different developmental stages, uninucleate microspores as well as mid-bicellular pollen. Starvation may also be a general trigger of pollen embryogenesis in higher plants, as it is effective not only in tobacco but also in other species such as barley (Hoekstra et al, 1992), wheat (Indrianto et al, 1999); rice (Ogawa et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Induction of Sporophytic Division in Orchids Microspores by Stress

Indrianto¹,

Siregar

Linuhung

et al. 2015

KLS

Self Cite

View full text Add to dashboard Cite

Orchid is one of the important ornamental plants in Indonesia this plant generally propagated by seed. Enhancing quality of this plant through breeding technology by various plant tissue culture methods and biotechnology, including doubled haploid technology are necessary. The most efficient method in creating doubled haploids plant is via microspore embryogenesis. We have develop new, innovative doubled haploid technology using the technique of isolated microspore culture. The goals are to obtain data on the male gametophyte development, viable embryogenic microspores, microspores derived embryos and double haploid plants of Orchid.Development of male gametophytes were analysed by isolation of microspores and pollen at various stages and staining with DAPI. Isolated orchid buds of Dendrobium hybrid 1, Vanda tricolor and Spathoglotis plicata were subjected to cold temperatures (4 o C) for 7 days, microspores were then isolated by crushing the pollinia using glass rod and cultured them in embryogenesis A2, NP, MS and VW medium, viability of the microspores were determine by using Flourescein diacetate (FDA). Isolated Orchid pollinia were cultured in starvation medium B at various temperatures and duration of time to evaluate embryogenic response, isolated microspores then were cultured further in the basic embryogenesis medium and incubated at 25 o C in the darkness.The result showed that floral characteristics for the late-uninucleate stage of the microspores were different for every orchid spesies. Ovulum lenght was used for Vanda, while in Dendrobium, Phalaenopsis, Arachnis, Spathoglottis plicata and Cattleya, varied length of flower bud was used. Isolated microspores of Dendrobium hybrid 1, Vanda tricolor and Spathoglotis plicata at 7 th days of culture in different media formulation showing different respond of viability. Medium A2 keeping viability of Dendrobium hybrid 1 microspores better than any other medium, while in Vanda tricolor and Spathoglotis plicata embryogenesis NP medium was superior. Incubation of orchid pollinia at 4 and 25 o C were successfully maintain viability of the microspores during starvation periods but not able to block gametophytic development. In contrast starved pollinia at 33 o C were succesfully block gametophytic development, percentage of embryogenic microspores after starvation of isolated pollinia at 33°C for 4 days was superior compare to any other treatments. Symmetrical divisions and some multicellular structures were observed, which were clear indication for the sporophytic development of microspore-derived embryos, they had developed and after a few weeks they degenerated and died.

show abstract

Section: Starvation Of Isolated Orchid Polliniamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Induction of Sporophytic Division in Orchids Microspores by Stress

Indrianto¹,

Siregar

Linuhung

et al. 2015

KLS

Self Cite

View full text Add to dashboard Cite

show abstract

“…These researchers verified the formation of embryos on surface of cultured anthers of Datura innoxia. Since then, microspore embryogenesis has been reported in more than 200 species of angiosperms (Indrianto et al, 1999). Double haploid plants obtained from anther culture are increasingly used in plant breeding for production of homozygous lines, but its application has been limited in many species mainly because androgenetic mechanism is largely unknown.…”

Section: Introductionmentioning

confidence: 99%

“…Four stress treatments have been shown to induce microspore embryogenesis: cold shock, heat shock, carbohydrate and nitrogen starvation and colchicine. Heat shock has been reported to increase the embryogenic route in several plants like wheat (Touraev et al, 1996c), tobacco (Touraev et al, 1996a and1996b) and Brassica (Telmer et al, 1995;Binarova et al, 1997;Custers et al, 1994) while the cold shock and starvation induced microspore embryogenesis in maize, wheat, barley and rice (Indrianto et al, 1999). The developmental stage of microspore at the time of culture initiation is of crucial importance, with late vacuolated microspore to early bicellular pollen grain being the responsive stages to heat stress (Pechan and Keller, 1988).…”

Section: Introductionmentioning

confidence: 99%

Effect of temperature shock on soybean microspore embryogenesis

Moraes

Bonadese-Zanettini

Callegari-Jacques

et al. 2004

Braz. arch. biol. technol.

View full text Add to dashboard Cite

show abstract

“…Cold pre-treatment has become an important technique to enhance the induction rate of embryos in barley (Kasha et al, 2001;Lazaridou et al, 2005;Lezin et al, 1996), wheat (Indrianto et al, 1999;Saidi et al, 1997), maize (Barnabas et al, 1999), and other important crops. In apple, cold pre-treatment was used extensively after the report of Wang and Ogata (1988), who described that 16 days of cold pre-treatment were used.…”

Section: Introductionmentioning

confidence: 99%

Effects of the Microspore Development Stage and Cold Pre-treatment of Flower Buds on Embryo Induction in Apple (Malus ^|^times; domestica Borkh.) Anther Culture

Zhang

Tsukuni

Ikeda

et al. 2013

J. Japan. Soc. Hort. Sci.

View full text Add to dashboard Cite

To establish better embryo induction procedures in anther culture of apple, the relationships between the microspore stage and the flower bud stage, and between embryo induction and the developmental stage of microspores during cold pre-treatment of flower buds were investigated. We classified the flower bud stages into four: "Tight cluster", "First pink", "Pink", and "Full pink". Each stage was defined by a morphological characteristic. The microspore stages were classified into seven: "tetrads", "early uninucleate", "miduninucleate", "late uninucleate", "early binucleate", "late binucleate", and "mature grain". The morphological characteristic in each stage was clarified. The "Tight cluster" flower bud stage mainly corresponded with the microspore stage of tetrad to mid-uninucleate. The "First pink" of the flower cluster mainly corresponded with the mid-uninucleate and late uninucleate microspore. The "Pink" and "Full pink" of flowering corresponded respectively with the late binucleate and the mature grain. Cold pre-treatment for more than 25 days of flower buds was found to be an efficient means of increasing embryo formation in anther culture. The microspore of late uninucleate and early binucleate stages grew into embryos efficiently after 25 days of cold pre-treatment. To achieve that condition, flower buds at late "Tight cluster" to early "First pink" stage were collected because the microspore stage progresses during 25 days of cold pre-treatment. Most microspores were early uninucleate to early binucleate stages at the "Tight cluster" to "First pink" flower bud stages. The shoots regenerated from embryos were analyzed using SSR markers. The investigation revealed that all shoots had a haploid origin.

show abstract

Assessment of various stresses and carbohydrates for their effect on the induction of embryogenesis in isolated wheat microspores

Cited by 64 publications

References 26 publications

Induction of Sporophytic Division in Orchids Microspores by Stress

Induction of Sporophytic Division in Orchids Microspores by Stress

Effect of temperature shock on soybean microspore embryogenesis

Effects of the Microspore Development Stage and Cold Pre-treatment of Flower Buds on Embryo Induction in Apple (Malus ^|^times; domestica Borkh.) Anther Culture

Contact Info

Product

Resources

About