Assessment of urinary total testosterone production by a highly sensitive time‐resolved fluorescence immunoassay

Bao, Shihua; Peng, Yifeng; Shen, Sheng; Lin, Qi

doi:10.1002/jcla.20283

Cited by 6 publications

(3 citation statements)

References 24 publications

(26 reference statements)

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“…In contrast to common procedure among anti-doping laboratories, E. coli ß glucuronidase hydrolyzed with dilute HCl was applied in order to deconjugate the sulphate metabolites together with glucuronide forms [30,31].…”

Section: Discussionmentioning

confidence: 99%

“…For hydrolysis, 4 mL 0.25 M HCl (0.25 M in methanol containing 8% water) were added and swirled carefully, followed by incubation of the tubes for one hour at 40 • C (heated water bath) under gentle shaking. The mild acidic pre-treatment conditions used similar to [28][29][30][31] resulted in deconjugation of both androgen sulphates and androgen glucuronides that are present in human urine.…”

Section: Sample Preparationmentioning

confidence: 99%

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Bioanalytical Detection of Steroid Abuse in Sports Based on the Androgenic Activity Measurement

Martín‐Escudero

Muñoz‐Guerra²,

García-Tenorio³

et al. 2021

Chemosensors

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The anabolic androgenic steroids (AAS) are the most frequently consumed performance enhancing drugs (PED) in sports. In the anti-doping field, the detection of AAS is carried out by the analysis of the athlete’s urine using methodologies based on liquid/gas chromatography-mass spectrometry. Unfortunately, the detection of unknown compounds is not possible. BDS’s AR CALUX® bio detection technology was studied as an indirect method to detect the administration of a single dose of testosterone (T). Twelve T and placebo single dose administered men volunteers underwent a triple-blind crossover clinical trial. The UGT2B17 deletion was present among the volunteers and evenly distributed in heterozygous (ins/del), wild-type homozygous (ins/ins), and mutated homozygous (del/del) groups. A significant statistical difference in terms of bioluminescence was observed after the testosterone (T) administration for the three types of polymorphic groups. The ratio of means between the pre- and post-T administration periods, depending on the type of polymorphism, was in group ins/ins 3.31 (CI. 95%: 2.07–5.29), group ins/del 4.15 (CI 95%: 3.05–5.67), and group del/del 2.89 (CI 95%: 2.42–3.46). The results of the study are very promising, as they may offer us the possibility of designing a detection approach that, based on intra-individual monitoring of androgenic values, in the UGT2B17 deletion type.

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Section: Discussionmentioning

confidence: 99%

Section: Sample Preparationmentioning

confidence: 99%

Bioanalytical Detection of Steroid Abuse in Sports Based on the Androgenic Activity Measurement

Martín‐Escudero

Muñoz‐Guerra²,

García-Tenorio³

et al. 2021

Chemosensors

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“…Urine is another sample type that can be used to assess reproductive hormone levels using radioimmunoassays (Bao, Peng, Sheng, & Lin, 2008). Importantly, urine samples do not capture hormone concentrations on shorter timescales, meaning this sample type is not well suited for studies interested in diurnal/pulsatile hormone patterns (Valeggia, 2007).…”

Section: Collection and Analysis Techniques: Urinementioning

confidence: 99%

Reproductive hormone measurement from minimally invasive sample types: Methodological considerations and anthropological importance

Gildner

2020

American J Hum Biol

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Energetic investment in human reproduction has long been recognized as costly, influencing developmental, physiological, and behavioral patterns in males and females. These effects are largely coordinated through the actions of reproductive hormones (eg, testosterone, estradiol, and progesterone). Here, the utility and limitations of minimally invasive sampling techniques are explored, providing a novel perspective on how reproductive hormone measurements can enhance reproductive endocrinology research. Salivary steroid measures are most commonly used, although several dried blood spot and urine assays are also available, and researchers continue to explore the efficacy of other sample types. These relatively simple measures have facilitated the collection of multiple samples from a single participant, allowing researchers to more accurately track the diurnal and cyclical variation exhibited by many reproductive hormones. Ultimately, the ability to collect fine‐grained participant data allows biological anthropologists to better test questions central to human reproductive ecology, life history theory, and public health. For example, fieldwork using these techniques suggests that testosterone profile variation across populations is influenced by energetic constraints and reproductive status. Moreover, hormone concentrations shape the development of sex characteristics, with implications for evolutionary questions related to sexual selection. Hormone levels also can be used to identify a range of medical concerns (eg, suppressed hormone production levels linked with psychosocial stress). These findings highlight how minimally invasive collection techniques can be applied to test diverse evolutionary hypotheses and identify important health concerns. Still, more work is needed to standardize collection and laboratory analysis procedures, thereby enabling more direct data comparisons between researchers.

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Profiling oestrogens and testosterone in human urine by stable isotope dilution/benchtop gas chromatography–mass spectrometry

et al. 2010

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Assessment of urinary total testosterone production by a highly sensitive time‐resolved fluorescence immunoassay

Cited by 6 publications

References 24 publications

Bioanalytical Detection of Steroid Abuse in Sports Based on the Androgenic Activity Measurement

Bioanalytical Detection of Steroid Abuse in Sports Based on the Androgenic Activity Measurement

Reproductive hormone measurement from minimally invasive sample types: Methodological considerations and anthropological importance

Profiling oestrogens and testosterone in human urine by stable isotope dilution/benchtop gas chromatography–mass spectrometry

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