Profiling oestrogens and testosterone in human urine by stable isotope dilution/benchtop gas chromatography–mass spectrometry

Hoffmann, P; Hartmann, Michaela F.; Remer, Thomas; Zimmer, Klaus–Peter; Wudy, Stefan A.

doi:10.1016/j.steroids.2010.06.014

Cited by 28 publications

(17 citation statements)

References 32 publications

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“…Although circulating estrogen is predominantly bound to a steroid binding protein, significant concentrations of estradiol and various metabolites are filtered and present in the urine. 17 We further find cortical GPR30 upregulation in the HS mRen2 female. It is not known whether salt directly affects GPR30 expression within the renal cortex or whether the increase in GPR30 reflects a compensatory mechanism of enhanced transcription or mRNA stabilization to oppose the damaging effects of salt within the kidney.…”

Section: Discussionsupporting

confidence: 51%

Estrogen Receptor GPR30 Reduces Oxidative Stress and Proteinuria in the Salt-Sensitive Female mRen2.Lewis Rat

et al. 2011

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The current study assessed whether activation of the novel estrogen receptor GPR30 ameliorates salt-dependent renal damage in intact mRen2.Lewis (mRen2) females. Hemizygous mRen2 were maintained on either a normal salt (NS, 0.5% Na) or high salt (HS, 4% Na) diet for 10 wks (5 to 15 wks of age), and HS animals were treated with the GPR30 agonist G-1 or vehicle for two weeks. Systolic blood pressure markedly increased with HS [149 ± 3 to 219 ± 5 mmHg, P<0.01], but G-1 did not influence pressure [P=0.42]. G-1 and estradiol induced relaxation of pre-constricted mesenteric vessels from NS mRen2, but both responses were attenuated in the HS group. Despite the lack of an effect on blood pressure, G-1 decreased renal hypertrophy, proteinuria, urinary 8-isoprostane excretion, and tubular 4-HNE staining. HS significantly increased GPR30 mRNA [1.01 ± 0.04 vs. 1.59 ± 0.13; P<0.01] and protein [0.60 ± 0.31 vs. 3.99 ± 0.75; P<0.01] in the renal cortex. GPR30 was highly expressed in the brush border of proximal tubules and co-localized with megalin. Finally, megalin expression was reduced by HS and restored with G-1. We conclude that GPR30-mediated beneficial effects in salt-sensitive mRen2 females occurred independent of changes in systolic blood pressure. The failure of G-1 to influence pressure may reflect a salt-induced impairment in GPR30-mediated vasorelaxation. The renoprotective actions of GPR30 may involve attenuation of tubular oxidative stress and activation of megalin-mediated protein reabsorption.

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Section: Discussionsupporting

confidence: 51%

Estrogen Receptor GPR30 Reduces Oxidative Stress and Proteinuria in the Salt-Sensitive Female mRen2.Lewis Rat

et al. 2011

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“…The LOQs in Milli-Q water were 0.01 ng/mL, which were ten times lower than the reported method by off-line SPE or online SPE analysis [15,19,26,33]. This was attributed to the combination of online SPE with large volume injection and high sensitive MS-MS spectrometry.…”

Section: Quality Control and Methods Performancementioning

confidence: 66%

“…Furthermore, AAS are even misused by athletes to improve their sports performance [6,15]. Although the use of AAS has been banned in sports and governed by the World Anti-Doping Agency since 1976, some of the priority controlled substances such as methenolone were still detected in urine at the [19,20] and LC-MS/MS [21][22][23]. Immunoassay is a feasible method in the clinical laboratory but it is susceptible to matrix interference especially for determination of low concentration.…”

Section: Introductionmentioning

confidence: 99%

Automated and sensitive determination of four anabolic androgenic steroids in urine by online turbulent flow solid-phase extraction coupled with liquid chromatography–tandem mass spectrometry: A novel approach for clinical monitoring and doping control

Guo

Shao²,

Liu

et al. 2014

Talanta

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“…In recent years, separation methods such as gas and liquid chromatography coupled with mass spectrometry are widely used due to their excellent sensitivity and high selectivity to analyze testosterone in biological matrices (e.g. urine, blood, serum, plasma, hair, saliva, nail) [11][12][13][14][15][16][17][18]. However, most of these approaches require expensive instrumentation, highly skilled personnel, and multiple-steps sample pretreatment procedures such as derivatization, extraction and purification, which would be tedious and timeconsuming.…”

Section: Introductionmentioning

confidence: 99%

Voltammetric Behavior of Testosterone on Bismuth Film Electrode: Highly Sensitive Determination in Pharmaceuticals and Human Urine by Square‐Wave Adsorptive Stripping Voltammetry

et al. 2015

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In this paper, an electrochemical application of bismuth‐film electrode (BiFE) fabricated via ex‐situ electrodeposition onto a glassy carbon electrode for testosterone determination was investigated in aqueous and aqueous/surfactant solutions. In cyclic voltammetry, the compound showed one irreversible and adsorption‐controlled reduction peak. The BiFE revealed good linear response in the examined concentration range of 1 to 45 nmol L−1 testosterone in BrittonRobinson buffer, pH 5.0 containing 3 mmol L−1 cetyltrimethylammonium bromide. The limit of detection was 0.3 nmol L−1 (0.09 ng mL−1). Finally, the BiFE was satisfactorily applied for quantitation of testosterone in both pharmaceutical (oil‐based ampoule) and biological (human urine) samples.

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Profiling oestrogens and testosterone in human urine by stable isotope dilution/benchtop gas chromatography–mass spectrometry

Cited by 28 publications

References 32 publications

Estrogen Receptor GPR30 Reduces Oxidative Stress and Proteinuria in the Salt-Sensitive Female mRen2.Lewis Rat

Estrogen Receptor GPR30 Reduces Oxidative Stress and Proteinuria in the Salt-Sensitive Female mRen2.Lewis Rat

Automated and sensitive determination of four anabolic androgenic steroids in urine by online turbulent flow solid-phase extraction coupled with liquid chromatography–tandem mass spectrometry: A novel approach for clinical monitoring and doping control

Voltammetric Behavior of Testosterone on Bismuth Film Electrode: Highly Sensitive Determination in Pharmaceuticals and Human Urine by Square‐Wave Adsorptive Stripping Voltammetry

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