Assessment of two complementary liquid chromatography coupled to high resolution mass spectrometry metabolomics strategies for the screening of anabolic steroid treatment in calves

Dervilly, Gaud; Weigel, Stefan; Lommen, Arjen; Chéreau, Sylvain; Rambaud, Lauriane; Essers, M.L.; Antignac, Jean‐Philippe; Nielen, Michel W. F.; Bizec, Bruno Le

doi:10.1016/j.aca.2011.02.008

Cited by 61 publications

(51 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Most of the applications reported in literature and dealing with anabolic practices detection in breeding animals through metabolomics have been conducted on urine samples and have involved normalization steps through lyophilization. [14,18] …”

Section: Sample Preparationmentioning

confidence: 99%

“…As no definitive and unique standard operation procedures currently exist, the comparative generation and analysis of data obtained from identical samples but acquired through different analytical conditions should help the interpretation of the information generated. Such developments relating to analysis and comparisons have recently been conducted in several laboratories, [12][13][14][15][16][17][18] leading to the formulation of in-house strategies for each of the steps of interest.…”

Section: Metabolomics Workflow To Highlight Biomarkers Of Anabolic Prmentioning

confidence: 99%

“…Typical reported protocols for the investigation of breeding animals' metabolome to highlight anabolic practices have involved at least ten animals divided into control and treated groups. [14][15][16][17][18] Regarding biological matrices of interest to be collected during the animal experiment, urine, serum or plasma are easy to collect as often as necessary and can be directly collected at the farm for doping control purposes. In addition, plasma and urine provide very complementary information about the state of an organism.…”

Section: Animal Experiments and Biological Samples Of Interestmentioning

confidence: 99%

“…Several approaches have shown potential to discriminate through analysis of urine samples of animals treated with a range of anabolic formulations: DHEA and pregnenolone in bovines, [13] clenbuterol in calves, [14] recombinant equine growth hormone in horses, [15][16][17] and nandrolone and estradiol in calves. [18] Metabolomics is an emerging field of 'omics' research that focuses, in an untargeted mode, on large-scale and high-throughput measurement of small molecules (so-called metabolites) in biological matrices. While metabonomics focuses on understanding systemic change through time in complex multicellular systems, [19] metabolomics refers to an analytical description of biological samples, the metabolome consisting in '.…”

Section: Introductionmentioning

confidence: 99%

“…The relevance and efficiency of this protocol has already been demonstrated through various applications dedicated to the screening of anabolic practices in cattle. [12,[14][15][16][17][18] In particular, the specificity related to the different biological matrices that may be used to perform a metabolomics study will be discussed.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Metabolomics in food analysis: application to the control of forbidden substances

Dervilly

Courant

Chéreau

et al. 2012

Drug Testing and Analysis

Self Cite

View full text Add to dashboard Cite

Metabolomics is a science of interest in food analysis to describe and predict properties of food products and processes. It includes the development of analytical methods with the ultimate goal being the identification of so-called 'quality markers', (i.e. sets of metabolites that correlate with, for example, quality, safety, taste, or fragrance of foodstuffs). In turn, these metabolites are influenced by factors as genetic differences of the raw food ingredients (such as animal breed or crop species differences), growth conditions (such as climate, irrigation strategy, or feeding) or production conditions (such as temperature, acidity, or pressure). In cases where the routine-based measurement of a food property faces some limitations such as the lack of knowledge regarding the target compounds to monitor, monitoring based on a limited set of crucial biomarkers is a good alternative, which is of great interest for food safety purposes regarding growth promoting practices. Such an approach may be more efficient than using a classic approach based on a limited set of known metabolites of anabolic compounds. In this context, screening strategies allowing detection of the physiological response resulting from anabolic compound administration are promising approaches to detect their misuse. The global metabolomics workflow implemented for such studies is presented and illustrated through various examples of biological matrices profiling (tissue, blood, urine) and for different classes of anabolic compounds (steroids, b-agonists and somatotropin).

show abstract

Section: Sample Preparationmentioning

confidence: 99%