Assessment of trophic transfer of benzo(a)pyrene genotoxicity from the post-larval pink shrimp F. brasiliensis to the juvenile Florida pompano T. carolinus

Rocha, Arthur José da Silva; Santos, Thaís da Cruz Alves dos; Gomes, Vicente; Bı́cego, Márcia Caruso; Albergaria-Barbosa, Ana Cecília Rizzatti de; Passos, Maria José de Arruda Campos Rocha; Hasue, Fabio Matsu; Ngan, Phan Van

doi:10.1016/j.etap.2012.07.010

Cited by 9 publications

(6 citation statements)

References 52 publications

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“…It is known, for example, that some genotoxicity tests are highly sensitive to DNA damage, even at reduced concentrations of pollutants (Monserrat et al, 2007). This fact has been confirmed in micronucleus tests on fish (Cavas et al, 2005;Deguchia et al, 2007;Adam et al, 2010;Sponchiado et al, 2011;Arslan et al, 2015), mollusks (BURGEOT et al, 1995Eskandari et al, 2012;Sacchi et al, 2013;Kandroo et al, 2015), and crustaceans (Fossi, 2000;Nudi et al, 2010;Klobučar et al, 2012;Rocha et al, 2012;Pinheiro et al, 2013), even when the exposure time to a xenobiotic was 24 h or less.…”

Section: Discussionmentioning

confidence: 91%

Multi-level biological responses in Ucides cordatus (Linnaeus, 1763) (Brachyura, Ucididae) as indicators of conservation status in mangrove areas from the western atlantic

Duarte

Souza

Nobre

et al. 2016

Ecotoxicology and Environmental Safety

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There is a global lack of knowledge on tropical ecotoxicology, particularly in terms of mangrove areas. These areas often serve as nurseries or homes for several animal species, including Ucides cordatus (the uçá crab). This species is widely distributed, is part of the diet of human coastal communities, and is considered to be a sentinel species due to its sensitivity to toxic xenobiotics in natural environments. Sublethal damages to benthic populations reveal pre-pathological conditions, but discussions of the implications are scarce in the literature. In Brazil, the state of São Paulo offers an interesting scenario for ecotoxicology and population studies: it is easy to distinguish between mangroves that are well preserved and those which are significantly impacted by human activity. The objectives of this study were to provide the normal baseline values for the frequency of Micronucleated cells (MN‰) and for neutral red retention time (NRRT) in U. cordatus at pristine locations, as well to indicate the conservation status of different mangrove areas using a multi-level biological response approach in which these biomarkers and population indicators (condition factor and crab density) are applied in relation to environmental quality indicators (determined via information in the literature and solid waste volume). A mangrove area with no effects of impact (areas of reference or pristine areas) presented a mean value of MN‰<3 and NRRT>120min, values which were assumed as baseline values representing genetic and physiological normality. A significant correlation was found between NRRT and MN, with both showing similar and effective results for distinguishing between different mangrove areas according to conservation status. Furthermore, crab density was lower in more impacted mangrove areas, a finding which also reflects the effects of sublethal damage; this finding was not determined by condition factor measurements. Multi-level biological responses were able to reflect the conservation status of the mangrove areas studied using information on guideline values of MN‰, NRRT, and density of the uçá crab in order to categorize three levels of human impacts in mangrove areas: PNI (probable null impact); PLI (probable low impact); and PHI (probable high impact). Results confirm the success of U. cordatus species' multi-level biological responses in diagnosing threats to mangrove areas. Therefore, this species represents an effective tool in studies on mangrove conservation statuses in the Western Atlantic.

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Section: Discussionmentioning

confidence: 91%

Multi-level biological responses in Ucides cordatus (Linnaeus, 1763) (Brachyura, Ucididae) as indicators of conservation status in mangrove areas from the western atlantic

Duarte

Souza

Nobre

et al. 2016

Ecotoxicology and Environmental Safety

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“…Rocha et al (2012) showed that this fish suffered significant damage to its DNA after being fed with shrimp exposed to benzo(a)pyrene concentrations, thus reinforcing its usefulness for genotoxicity studies.…”

Section: Discussionmentioning

confidence: 99%

“…This feeding habit can contribute to the ingestion of many organisms that may have been previously contaminated. Moreover, it is being used as a sentinel organism for genotoxicity of PAHs and other pollutants in our laboratory (SANTOS et al, 2006;ROCHA et al, 2012). The aim of the present study was to evaluate the DNA damage in juvenile T. carolinus exposed to different concentrations of anthracene for a specific period then subsequently allowed to depurate in clean water for different periods of time using the comet assay.…”

Section: Introductionmentioning

confidence: 99%

Assessment of genotoxicity and depuration of anthracene in the juvenile coastal fish Trachinotus carolinus using the comet assay

et al. 2013

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A B S T R A C TIn the environment, anthracene is characterized as being persistent, bioaccumulative and toxic to aquatic organisms. Biotransformation of xenobiotic substances, such as anthracene, produces reactive oxygen species that may induce DNA strand breaks. The aim of the present study was to evaluate the DNA damage in juvenile T. carolinus exposed to different concentrations (8, 16 and 32 µg.L -1 ) of anthracene for 24 h in the dark then subsequently allowed to depurate in clean water for different periods of time (48, 96 or 144 h) using the comet assay. Our results show that anthracene is genotoxic to T. carolinus and that DNA damage was dose-and depuration/time-dependent. Anthracene genotoxicity was observed in all experimental concentrations. Depuration seemed to be more efficient in fish exposed to the lowest anthracene concentration and maintained in clean water for 96 h. R E S U M OAmbientalmente, o antraceno é considerado persistente, bioacumulativo e tóxico para organismos aquáticos. A biotransformação de substâncias xenobióticas, como o antraceno, produz espécies reativas de oxigênio que podem agir sobre o DNA. O objetivo deste trabalho foi avaliar o dano ao DNA em T. carolinus expostos a diferentes concentrações de antraceno (8, 16 e 32 µg.L -1 ) por 24 h no escuro e subsequentemente mantidos em água limpa para depuração por três diferentes períodos de tempo (48, 96 e 144 h) através de ensaio cometa. Os resultados obtidos demonstram que o antraceno é genotóxico para T. carolinus e o dano ao DNA foi dose-e tempo de depuração-dependente. A genotoxicidade do antraceno foi detectada nas três concentrações utilizadas. A depuração pareceu ser mais eficiente nos peixes expostos a menor concentração de antraceno e mantidos por 96 h em água limpa.

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“…Danos ao material genético já foram associados à redução do crescimento, anormalidades no desenvolvimento e diminuição na sobrevivência de larvas, juvenis e adultos de uma grande variedade de organismos aquáticos (LEE; STEINERT, 2003), fatores fundamentais para o bom desenvolvimento de uma criação. O ensaio cometa é um método muito utilizado para a detecção desses danos por ser relativamente rápido, de baixo custo, alta sensibilidade e requerer um pequeno número de células ROCHA et al, 2012;HASUE et al, 2013). Basicamente, ele consiste em uma eletroforese que separa o DNA intacto daquele fragmentado por algum agente estressor.…”

Section: Parte I: Avaliação Do Estado Fisiológico De Juvenis De Beijuunclassified

Biomarcadores na avaliação do estado fisiológico de juvenis de beijupirás, <i>Rachycentron canadum</i> (Linnaeus, 1766), de cultivo.

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Assessment of trophic transfer of benzo(a)pyrene genotoxicity from the post-larval pink shrimp F. brasiliensis to the juvenile Florida pompano T. carolinus

Cited by 9 publications

References 52 publications

Multi-level biological responses in Ucides cordatus (Linnaeus, 1763) (Brachyura, Ucididae) as indicators of conservation status in mangrove areas from the western atlantic

Multi-level biological responses in Ucides cordatus (Linnaeus, 1763) (Brachyura, Ucididae) as indicators of conservation status in mangrove areas from the western atlantic

Assessment of genotoxicity and depuration of anthracene in the juvenile coastal fish Trachinotus carolinus using the comet assay

Biomarcadores na avaliação do estado fisiológico de juvenis de beijupirás, <i>Rachycentron canadum</i> (Linnaeus, 1766), de cultivo.

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