Assessment of tissue glutathione status during experimental sepsis

Breuillé, Denis; Malmezat, T.; Rose, Felicity R. A. J.; Pouyet, Corinne; Obled, Christiane

doi:10.1016/0261-5614(94)90131-7

Cited by 7 publications

(4 citation statements)

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“…It appears that, in our experimental conditions, glutathione concentrations were probably not a good indicator of the impact of poor sanitary conditions on weaned pigs. In rats suffering from severe inflammations, Breuillé et al (1994) showed that tissue and blood glutathione concentrations increased during the first day after the experimental infection before decreasing during the later stages of infection. Indeed, gluthathione concentrations can depend on the balance between glutathione synthesis and its utilization as a free radical scavenger (Malmezat et al, 2000).…”

Section: Discussionmentioning

confidence: 98%

The effect of sanitary status degradation and dietary tryptophan content on growth rate and tryptophan metabolism in weaning pigs1

Floc'H

LeBellego²,

Matte

et al. 2009

Journal of Animal Science

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Health degradation modifies Trp metabolism through induction of Trp catabolism. This could limit the amount of Trp available for growth. The aims of the present experiment were to investigate the effects of a low grade inflammation and dietary Trp on growth and Trp metabolism. Eighty weaned pigs were assigned to 4 experimental treatments according to a 2 x 2 factorial arrangement: 2 sanitary statuses x 2 dietary Trp contents. The Trp content was deficient (low-Trp: 2.4 and 1.9 g of Trp/kg of the phase I and phase II diets, respectively) or adequate (high-Trp: 2.9 and 2.4 g of Trp/kg of the phase I and phase II diets, respectively). A low grade inflammatory response was induced by housing pigs in unsanitary environment, whereas control pigs were housed in good sanitary conditions. Pigs were not fed ad libitum to avoid feed refusals. Growth performance was calculated 3, 5, and 7 wk after weaning. Blood was sampled 12, 33, and 47 d after weaning for the determination of plasma concentrations of Trp and related metabolites, kynurenine and pyridoxal-5-phosphate. The interaction between sanitary status and dietary Trp was not statistically significant in all measured criteria. Pigs kept in poor sanitary conditions grew slower (P < 0.001) during the entire experimental period and had greater plasma concentrations of haptoglobin (P < 0.001) than pigs housed in good sanitary conditions. Pigs housed in poor sanitary conditions had also decreased Trp plasma concentrations (P < 0.001), but plasma kynurenine concentrations were not affected. Our results indicated that a moderate inflammatory response was obtained by degrading the sanitary quality of environment. Additionally, poor sanitary conditions modified Trp metabolism, indicating that the amount of Trp available for growth and other metabolic functions might be reduced.

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Section: Discussionmentioning

confidence: 98%

The effect of sanitary status degradation and dietary tryptophan content on growth rate and tryptophan metabolism in weaning pigs1

Floc'H

LeBellego²,

Matte

et al. 2009

Journal of Animal Science

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“…Thus, as we already suggested (Mayot et al, 2007), albumin may not be a good nutritional marker in old rats. Glutathione which is known to be very sensitive to protein intake (Hum et al, 1992) and to decrease during infection (Breuillé et al, 1994) was highly depleted in the stressed old rats. The overall effect of the present stress applied in old rats on plasma levels of acute phase proteins and liver glutathione status was largely consistent with known effects of protein-deficient nutrition in young animals, albumin excepted.…”

Section: Accepted Manuscriptmentioning

confidence: 94%

Presence of low-grade inflammation in old rats does not worsen skeletal muscle loss under an endotoxemic and dietary stress

Mayot¹,

Vidal²,

Combaret³

et al. 2007

Experimental Gerontology

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The study aimed to determine if age-associated low-grade inflammation aggravates the response to a stress, especially regarding to sarcopenia. Initial inflammatory status in 22-month-old rats was based on plasma alpha(2)-macroglobulin and fibrinogen concentrations. The stress applied was a single intra-peritoneal injection of lipopolysaccharide followed by a 23-day period of malnutrition, i.e. a 4% casein diet distributed in quantity limited to 50% of spontaneous food intake. The response to the stress was analyzed in non-inflamed and low-grade inflamed rats and compared to non-inflamed and low-grade inflamed rats, which received the control treatment (i.e. no lipopolysaccharide injection and an 18% casein diet). The stress-induced body weight loss was higher in inflamed than non-inflamed rats, but the decrease in muscle weight was not worsened. Muscle protein turnover was not affected by the stress. Plasma alpha(2)-macroglobulin levels increased after the stress, whatever the initial inflammatory status. However, fibrinogen levels decreased more in inflamed than non-inflamed rats and albumin levels were not affected by the stress. Independently of the initial inflammatory status, the liver glutathione content was strongly depleted by the stress. These results extend and support our previous findings by demonstrating that age-associated low-grade inflammation does not aggravate sarcopenia in old rats.

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“…In contrast, during the first days following infection, tissue GSH levels are elevated in treated animals compared with controls (41,42). However, in the later stages of infection, depletion of tissue GSH levels occurs (43,44).…”

Section: Discussionmentioning

confidence: 92%

“…Breuille et al (43) have reported that in spite of strong anorexia, GSH concentration of septic rats was greater than pair-fed rats in nearly all tissues, only blood GSH level was decreased, liver GSH concentrations were even greater than the values that had measured in healthy fed rats and suggested that the inflammatory challenge overcomes the influence of food restriction. Others have described the unchanged or increased GSH status in different tissues 24 and 48 h after induction of stress in animal models (41,46,48).…”

Section: Discussionmentioning

confidence: 96%