Assessment of the response of <i>Plasmodiophora brassicae</i> in contaminated horticultural land, using lime‐based fertilizer concentrations

Faggian, Robert; Hanson, Mary; Kennedy, Roy; Petch, Geoffrey; Wakeham, Alison

doi:10.1002/fes3.122

Cited by 6 publications

(4 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…With the development of high-throughput sequencing technology, it has become possible to explore dynamic changes in microbial communities with the application of biological control agents [ 15 , 33 , 34 , 35 , 36 ]. Furthermore, the use of quantitative polymerase chain reaction (qPCR) based technologies enables quantification of microorganisms in soil [ 8 , 37 , 38 , 39 , 40 ]. These methods were used in this study to facilitate the assessment of Trichoderma biological control and its impact upon the P. brassicae population.…”

Section: Introductionmentioning

confidence: 99%

Trichoderma harzianum Inoculation Reduces the Incidence of Clubroot Disease in Chinese Cabbage by Regulating the Rhizosphere Microbial Community

Philp

et al. 2020

Microorganisms

View full text Add to dashboard Cite

Clubroot is a disease of cruciferous crops that causes significant economic losses to vegetable production worldwide. We applied high-throughput amplicon sequencing technology to quantify the effect of Trichodermaharzianum LTR-2 inoculation on the rhizosphere community of Chinese cabbage (Brassica rapa subsp. pekinensis cv. Jiaozhou) in a commercial production area. T. harzianum inoculation of cabbage reduced the incidence of clubroot disease by 45.4% (p < 0.05). The disease control efficacy (PDIDS) was 63%. This reduction in disease incidence and severity coincided with a drastic reduction in both the relative abundance of Plasmodiaphora brassicae, the causative pathogen of cabbage clubroot disease, and its copy number in rhizosphere soil. Pathogenic fungi Alternaria and Fusarium were also negatively associated with Trichoderma inoculation according to co-occurrence network analysis. Inoculation drastically reduced the relative abundance of the dominant bacterial genera Delftia and Pseudomonas, whilst increasing others including Bacillus. Our results demonstrate that T. harzianum LTR-2 is an effective biological control agent for cabbage clubroot, which acts through modulation of the soil and rhizosphere microbial community.

show abstract

Section: Introductionmentioning

confidence: 99%

Trichoderma harzianum Inoculation Reduces the Incidence of Clubroot Disease in Chinese Cabbage by Regulating the Rhizosphere Microbial Community

Philp

et al. 2020

Microorganisms

View full text Add to dashboard Cite

show abstract

“…To date, Verticillium stripe has been identified in Europe [37,38], North America [2,39], and Asia [40], where another significant disease of crucifers, clubroot, is also frequently reported [41]. Since clubroot development is favored in acidic soils, liming to increase soil pH is often recommended as a method for controlling this disease [41][42][43][44]. However, liming should be reconsidered in fields or regions where Verticillium stripe or other diseases favored by alkaline environments are also an issue.…”

Section: Discussionmentioning

confidence: 99%

Influence of pH on the Growth of Verticillium longisporum and Verticillium Stripe Severity in Canola (Brassica napus)

Wang,

Strelkov,

Hwang

2024

Horticulturae

View full text Add to dashboard Cite

Verticillium stripe, caused by Verticillium longisporum, is an emerging disease of canola (Brassica napus) in Canada. Studies were conducted to assess the impact of pH on both the growth of V. longisporum and its virulence on the canola host. Fungal growth was assessed by measuring the colony diameter following 14 and 21 days of incubation on potato dextrose agar at varying pH levels (4.7, 5.5, 6.5, 7.4, or 8.6). The results indicated that colonies of V. longisporum were approximately 16% greater in diameter at pH 7.4 and 8.6 compared with those at pH 5.5. The impact of pH on disease development at the seedling stage was investigated using a semi-hydroponic system with different pH levels of 4.4, 5.4, 6.3, 7.5, and 8.4 in half-strength Hoagland’s solution. Verticillium stripe was most severe at pH 7.5 and 8.4 after a 10-day period in the semi-hydroponic system. In a second inoculation experiment, canola seedlings previously inoculated with the fungus were transplanted into potting mix amended to four pH levels (5.6, 6.4, 7.2, and 7.8). The transplants were cultivated under greenhouse conditions and evaluated for Verticillium stripe severity at plant maturity. Disease severity was greatest at pH 7.8. This is the first study on the effects of pH on V. longisporum in canola. It suggests a substantial risk of increased disease severity and yield losses due to Verticillium stripe in regions with neutral to slightly alkaline soils.

show abstract

“…Traditional clubroot control methods use crop rotations and lime treatment [8][9][10]. Breeding resistant cultivars however is the primary clubroot management technique, although several recent strains are now exhibiting resistance [11].…”

Section: Introductionmentioning

confidence: 99%

Production of Clubroot Standards Using a Recombinant Surrogate to Overcome Natural Genetic Variability

Patel

Kennedy

2023

Plants

Self Cite

View full text Add to dashboard Cite

Clubroot is caused by the obligate pathogen Plasmodiophora brassicae. The organism targets root hair cells for entry and forms spores in numbers so large that they eventually develop characteristic galls or clubs on the roots. Clubroot incidence is rising globally and impacting the production of oil seed rape (OSR) and other economically important brassica crops where fields are infected. P. brassicae has a wide genetic diversity, and different isolates can vary in virulence levels depending on the host plant. Breeding for clubroot resistance is a key strategy for managing this disease, but identifying and selecting plants with desirable resistance traits are difficult due to the symptom recognition and variability in the gall tissues used to produce clubroot standards. This has made the accurate diagnostic testing of clubroot challenging. An alternative method of producing clubroot standards is through the recombinant synthesis of conserved genomic clubroot regions. This work demonstrates the expression of clubroot DNA standards in a new expression system and compares the clubroot standards produced in a recombinant expression vector to the standards generated from clubroot-infected root gall samples. The positive detection of recombinantly produced clubroot DNA standards in a commercially validated assay indicates that recombinant clubroot standards are capable of being amplified in the same way as conventionally generated clubroot standards. They can also be used as an alternative to standards generated from clubroot, where access to root material is unavailable or would take great effort and time to produce.

show abstract

Assessment of the response of Plasmodiophora brassicae in contaminated horticultural land, using lime‐based fertilizer concentrations

Cited by 6 publications

References 35 publications

Trichoderma harzianum Inoculation Reduces the Incidence of Clubroot Disease in Chinese Cabbage by Regulating the Rhizosphere Microbial Community

Trichoderma harzianum Inoculation Reduces the Incidence of Clubroot Disease in Chinese Cabbage by Regulating the Rhizosphere Microbial Community

Influence of pH on the Growth of Verticillium longisporum and Verticillium Stripe Severity in Canola (Brassica napus)

Production of Clubroot Standards Using a Recombinant Surrogate to Overcome Natural Genetic Variability

Contact Info

Product

Resources

About