Assessment of the potential genotoxic risk of Phyllantus orbicularis HBK aqueous extract using in vitro and in vivo assays

Sánchez-Lamar, Ángel; Fuentes, Jorge Luı́s; Fonseca, Gladys; Cápiro, Nancy; Ferrer, Mirle; Alonzo, Alena; Baluja, Ligia; Cozzi, Renata; Salvia, R. De; Fiore, Mario; Llagostera, Montserrat

doi:10.1016/s0378-4274(02)00255-2

Cited by 14 publications

(5 citation statements)

References 24 publications

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“…Many studies have shown positive results for the mutagenic action of plant extracts (Sánchez-Lamar et al, 2002;Zanoni et al, 2005). Studies such as ours indicate the importance of scientifically evaluating the security of the products used in popular medicine, especially those derived from plants.…”

Section: ***mentioning

confidence: 73%

Mutagenicity of the Musa paradisiaca (Musaceae) fruit peel extract in mouse peripheral blood cells in vivo

Andrade

Perazzo²,

Maistro³

2008

Genet. Mol. Res.

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ABstrACt. Plants are a source of many biologically active products and nowadays they are of great interest to the pharmaceutical industry. In the present study, the mutagenic potential of the Musa paradisiaca fruit peel extract was assessed by the single-cell gel electrophoresis (SCGE) and micronucleus assays. Animals were treated orally with three different concentrations of the extract (1000, 1500, and 2000 mg/kg body weight). Peripheral blood cells of Swiss mice were collected 24 h after treatment for the SCGE assay and 48 and 72 h for the micronucleus test. The results showed that the two higher doses of the extract of M. paradisiaca induced statistically significant increases in the average numbers of DNA damage in peripheral blood leukocytes for the two higher doses and a significant increase in the mean of micronucleated polychromatic erythrocytes in the three doses tested. The polychromatic/ normochromatic erythrocyte ratio scored in the treated groups was not statistically different from the negative control. The data obtained indicate that fruit peel extract from M. paradisiaca showed mutagenic effect in the peripheral blood cells of Swiss albino mice.

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Section: ***mentioning

confidence: 73%

Mutagenicity of the Musa paradisiaca (Musaceae) fruit peel extract in mouse peripheral blood cells in vivo

Andrade

Perazzo²,

Maistro³

2008

Genet. Mol. Res.

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“…Eukaryotic organisms: Saccharomyces cerevisiae. The most widely used Saccharomyces cerevisiae assay, proposed by Zimmermann et al in 1975(Sanchez-Lamar et al, 2002Nohynek et al, 2004), relies on the diploid D7 strain. When compounds mutate the DNA of this yeast strain, easily scorable phenotypes (color of colonies, growth on particular media) are produced at three separate genomic sites.…”

Section: In Vitro Methods For Genotoxicity Assessmentmentioning

confidence: 99%

“…Nonetheless, some herbal products and their secondary metabolites were assessed for genotoxicity by various techniques. The Ames test has been widely used to assess the mutagenicity of herbal products, including for example extracts from Calendula officinalis L., Echinodorus macrophyllus (Kunth) Micheli, Mouriri pusa Gardner, Phyllantus orbicularis Kunth, Punica granatum L., Parthenium hysterophorus L. and a green tea catechins preparation (Ramos et al, 1998(Ramos et al, , 2001Sanchez-Lamar et al, 2002;Santos et al, 2002;Nohynek et al, 2004). Among these extracts, Punica granatum whole fruit extract and the enriched fractions of flavonoids and tannins from Mouriri pusa were found to give a positive genotoxic test.…”

Section: Applications To Herbal Medicinesmentioning

confidence: 99%

Review of current and “omics” methods for assessing the toxicity (genotoxicity, teratogenicity and nephrotoxicity) of herbal medicines and mushrooms

Ouédraogo

Baudoux

Stévigny

et al. 2012

Journal of Ethnopharmacology

122

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“…Generally, these compounds react with cellular macromolecules, including DNA, causing cellular toxicity and/or genotoxicity (Rietjens et al, 2005a). A 'genotoxin' is a chemical or agent that can cause DNA or chromosomal damage (Phillips and Arlt, 2009), and 'genotoxicity' refers to potentially harmful effects on genetic material which are related to the induction of permanent transmissible changes in the amount or structure of the genetic material (Cavalcanti et al, 2010;Ogura et al, 2008;Sanchez-Lamar et al, 2002). Genotoxicity, broadly defined as 'damage to the genome', is a distinct and important type of toxicity as specific genotoxic events are considered hallmarks of cancer (Ellinger-Ziegelbauer et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Potential Genotoxicity of Traditional Chinese Medicinal Plants and Phytochemicals: An Overview

Zhou

Ouédraogo

et al. 2013

Phytotherapy Research

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In the last decades, cases of poisoning due to herbal medicines have occurred in many countries; Chinese herbal medicines (CHMs) are occasionally involved. The experience gained from traditional use is efficient to detect immediate or near-immediate relationship between administration and toxic effects but is quite unlikely to detect medium- to long-term toxicities; thorough investigations of herbal medicines (toxicity assessments, active pharmacovigilance) appear then essential for their safe use. Genotoxicity is an especially insidious toxicity that may result in carcinoma development years after exposure; it can arise from multiple compounds, with or without metabolic activation. The present work reviews traditional CHMs and phytochemicals that have been shown to present a genotoxic hazard.

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Assessment of the potential genotoxic risk of Phyllantus orbicularis HBK aqueous extract using in vitro and in vivo assays

Cited by 14 publications

References 24 publications

Mutagenicity of the Musa paradisiaca (Musaceae) fruit peel extract in mouse peripheral blood cells in vivo

Mutagenicity of the Musa paradisiaca (Musaceae) fruit peel extract in mouse peripheral blood cells in vivo

Review of current and “omics” methods for assessing the toxicity (genotoxicity, teratogenicity and nephrotoxicity) of herbal medicines and mushrooms

Potential Genotoxicity of Traditional Chinese Medicinal Plants and Phytochemicals: An Overview

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