Mutagenicity of the Musa paradisiaca (Musaceae) fruit peel extract in mouse peripheral blood cells in vivo

Abstract

ABstrACt. Plants are a source of many biologically active products and nowadays they are of great interest to the pharmaceutical industry. In the present study, the mutagenic potential of the Musa paradisiaca fruit peel extract was assessed by the single-cell gel electrophoresis (SCGE) and micronucleus assays. Animals were treated orally with three different concentrations of the extract (1000, 1500, and 2000 mg/kg body weight). Peripheral blood cells of Swiss mice were collected 24 h after treatment for the SCGE assay and 48 and 72 h for the micronucleus test. The results showed that the two higher doses of the extract of M. paradisiaca induced statistically significant increases in the average numbers of DNA damage in peripheral blood leukocytes for the two higher doses and a significant increase in the mean of micronucleated polychromatic erythrocytes in the three doses tested. The polychromatic/ normochromatic erythrocyte ratio scored in the treated groups was not statistically different from the negative control. The data obtained indicate that fruit peel extract from M. paradisiaca showed mutagenic effect in the peripheral blood cells of Swiss albino mice.

Evaluation of Commonly Used Surinamese Medicinal Plants for Their Potential Cytotoxic and Genotoxic Effects Using Cultured Chinese Hamster Ovary Cells

“…extract concerns, the relatively low cytotoxicity towards the CHO cells was in the range of that elicited by triterpenes and sterols from an ethanolic peel extract against cultured RAW 264.7 murine macrophages and LLC-MK2 rhesus monkey kidney epithelial cells [46]. And comparably to the current results, an paradisiaca peel extract produced significantly more comet images but not more micronuclei in the peripheral blood cells of Swiss mice treated orally with this preparation when compared to untreated animals [47].…”

Laurus nobilis L.: assessment of the cytotoxic and genotoxic potential of aqueous extracts by micronucleus and Allium cepa assays

“…The number of animals used in these experiments sought to comply with the standards of the Ethics Committee on Animal Use of the Biological Sciences and Health Institute of UFRRJ and to conform to the Principle of the 3R's, besides accompanying the recent references of other authors (Andrade, Perazzo, Maistro, 2008a;Andrade et al, 2008b;Agrawal, Pandely, 2009;Alves et al 2014).…”

“…The micronucleus assay was performed according to Andrade, Perazzo and Maistro (2008a), with modifications. Assays were carried out with 6 animals/ group and with the exception of the positive control, the treatment was performed intragastrically.…”

A review on banana flower: Nutritional composition, processed products and health benefits

“…Banana blossom is considered as an indigenous food and its consumptions is limited due to the tedious preparations procedure. [1][2][3][4][5][6][7][8] The banana blossoms are good sources of minerals. The samples were digested in HNO3/HClO4 for mineral determination.…”