Assessment of the Plasma/Serum IgG Test to Screen for Periodontitis

Abstract

Chronic periodontitis is a silent infectious disease prevalent worldwide and affects lifestyle-related diseases. Therefore, efficient screening of patients is essential for general health. This study was performed to evaluate prospectively the diagnostic utility of a blood IgG antibody titer test against periodontal pathogens. Oral examination was performed, and IgG titers against periodontal pathogens were measured by ELISA in 1,387 individuals. The cut-off value of the IgG titer was determined in receiver operating characteristic curve analysis, and changes in periodontal clinical parameters and IgG titers by periodontal treatment were evaluated. The relationships between IgG titers and severity of periodontitis were analyzed. The best cut-off value of IgG titer against Porphyromonas gingivalis for screening periodontitis was 1.682. Both clinical parameters and IgG titers decreased significantly under periodontal treatment. IgG titers of periodontitis patients were significantly higher than those of healthy controls, especially in those with sites of probing pocket depth over 4 mm. Multiplied cut-off values were useful to select patients with severe periodontitis. A blood IgG antibody titer test for Porphyromonas gingivalis is useful to screen hitherto chronic periodontitis patients.

Interdisciplinary orthodontic treatment for a patient with generalized aggressive periodontitis: Assessment of IgG antibodies to identify type of periodontitis and correct timing of treatment

“…In addition to the periodontal examination, a blood IgG antibody titer assay for periodontal pathogens with an enzyme-linked immunosorbent assay (ELISA), as previously described, was used to assess the degree of periodontal disease based on the detection of humoral immunologic responses accompanying the elevation in the serum IgG antibody titers against periodontal pathogens. 11,12 The results showed that the IgG antibody titer against A actinomycetemcomitans Y4, the B strain of A actinomycetemcomitans, which is frequently associated with AgP, was 4.85 times higher than the mean of 2 SD of the values observed in the healthy controls in a previous report (Fig 1, C). 12 The patient was therefore diagnosed with generalized AgP, possibly caused by A actinomycetemcomitans Y4.…”

Analysis of the relationship between periodontal disease and atherosclerosis within a local clinical system: a cross-sectional observational pilot study

“…To assess normal distribution, the Shapiro-Wilk test was used. The cut-off value of the titer obtained from the area under the receiver operating characteristic (ROC) curve [15] was set to 29, 39, and 49 the basis of a value of 1.68. The relation between atherosclerosis-related clinical markers in systemic examinations and severity of plasma IgG antibody level to P. gingivalis at baseline was analyzed by Student's t test for parametric data and Wilcoxon rank-sum test for non-parametric data using the groups by the individual cut-off values.…”

“…A blood sample was taken to ascertain Plasma IgG antibody titer to P. gingivalis FDC381. Blood sampling utilized the Plasma Antibody Titer Tests Using DEMECAL Ò Blood Test Set (Sunstar Inc. Osaka, Japan), and data were measured by Leisure, Inc. (Tokyo, Japan) by enzymelinked immunosorbent assay (ELISA) [15].…”

“…Thirty-one percent of the 89 subjects were obese (BMI % of PPD [4 mm at baseline were 29.9 ± 31.2 %, 3.5 ± 1.2 mm, and 40.8 ± 28.9 %, respectively. Fiftyseven percent of the 90 subjects had plasma IgG antibody titer to P. gingivalis [1.68 (cut-off value of the titer obtained from the area under the ROC curve [15]. The other examination results are shown in Table 1.…”

Prevalence and risk factors for peri-implant diseases in Japanese adult dental patients

“…IgG titers against A. actinomycetemcomitans, P. intermedia, Eikenella corrodens (E. c.), and P. gingivalis were determined using an enzyme-linked immunosorbent assay. The details of the measurement method are described in detail elsewhere (33).…”