Assessment of the phytochemical constituents and antioxidant activity of a bloom forming microalgae Euglena tuba

Chaudhuri, Dipankar; Ghate, Nikhil Baban; Deb, Sankha; Panja, Sourav; Sarkar, Rhitajit; Rout, Jayashree; Mandal, Nripendranath

doi:10.1186/0717-6287-47-24

Cited by 35 publications

(30 citation statements)

References 34 publications

(48 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the industrial wastewater we used in this study the majority of nitrogen is in the form of ammonia. Among the above nitrogen forms, ammonium is the most preferred form of nitrogen source for some microalgae in part because its uptake and utilization by microalgae is most energy efficient [ 11 , 21 ]. It is commonly accepted that nitrogen metabolism is linked to carbon metabolism in algae because they share organic carbon and energy supplied directly from photosynthetic electron transport and CO2 fixation as well as from the metabolic pathway of organic carbon [ 23 , 46 ].…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Carotenoids, Phenolic Compounds and Tocopherols Contribute to the Antioxidative Properties of Some Microalgae Species Grown on Industrial Wastewater

Safafar

Wagenen

Møller³

et al. 2015

Marine Drugs

348

185

View full text Add to dashboard Cite

This study aimed at investigating the potential of microalgae species grown on industrial waste water as a new source of natural antioxidants. Six microalgae from different classes, including Phaeodactylum sp. (Bacillariophyceae), Nannochloropsis sp. (Eustigmatophyceae), Chlorella sp., Dunaniella sp., and Desmodesmus sp. (Chlorophyta), were screened for their antioxidant properties using different in vitro assays. Natural antioxidants, including pigments, phenolics, and tocopherols, were measured in methanolic extracts of microalgae biomass. Highest and lowest concentrations of pigments, phenolic compounds, and tocopherols were found in Desmodesmus sp. and Phaeodactylum tricornuotom microalgae species, respectively. The results of each assay were correlated to the content of natural antioxidants in microalgae biomass. Phenolic compounds were found as major contributors to the antioxidant activity in all antioxidant tests while carotenoids were found to contribute to the 1,1-diphenyl-2-picryl-hydrazil (DPPH) radical scavenging activity, ferrous reduction power (FRAP), and ABTS-radical scavenging capacity activity. Desmodesmus sp. biomass represented a potentially rich source of natural antioxidants, such as carotenoids (lutein), tocopherols, and phenolic compounds when cultivated on industrial waste water as the main nutrient source.

show abstract

Section: Resultsmentioning

confidence: 99%

“…There are only few published studies regarding the identification and quantification of phenolic composition in microalgae species [ 8 , 11 , 20 , 21 ]. Abd El-Baky et al .…”

Section: Introductionmentioning

confidence: 99%

Carotenoids, Phenolic Compounds and Tocopherols Contribute to the Antioxidative Properties of Some Microalgae Species Grown on Industrial Wastewater

Safafar

Wagenen

Møller³

et al. 2015

Marine Drugs

348

185

View full text Add to dashboard Cite

show abstract

“…Reducing the power of a compound is the significant indicator of its antioxidant activity (Liu et al, 2013). It is related to their electron transfer ability and they can act as primary and secondary antioxidants (Chaudhuri et al, 2014). An increase in the absorbance at 700 nm indicates high reducing ability.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant and antimitotic activities of sulfated polysaccharide from marine brown algae Padina tetrastromatica

Jose¹,

Radhakrishnan²,

Kurup³

2015

View full text Add to dashboard Cite

Antioxidants play a central role in the prevention of carcinogenesis. The most natural compounds exhibit their protective effects by eliciting antioxidant potential. Sulfated polysaccharide was isolated from the brown algae Padina tetrastromatica, then purified and evaluated for its composition and in vitro antioxidant and antimitotic activities. Both ethanolic sulfated polysaccharide (ESPS) and ethanolic sulfated polysaccharide-column purified (ESPS-CP) exhibited considerable amount of carbohydrates (11.2% and 17.6%), sulfate (11.4% and 7.4%), fucose (5.5% and 15.7%), uronic acid (4.7% and 11.8%), xylose (0.5% and 0.03%) and sulfated polysaccharide (2.4% and 12.7%) content. The FTIR analysis and phytochemical screening also confirmed the presence of sulfated polysaccharides. In the in vitro antioxidant activity determination using DPPH (1-1-diphenyl 2-picryl hydrazyl) radical scavenging activity, hydroxyl radical scavenging activity, superoxide anion scavenging activity, hydrogen peroxide scavenging activity, total antioxidant activity and reducing power, ESPS showed more activity than ESPS-CP. In the case of nitric oxide radical scavenging, ESPS-CP was found to be more effective. At a concentration of 2mg/ml, both samples were potent antioxidants with significant IC50 values. The antimitotic studies such as mitotic index in onion root tips and sprouting assay in green gram seeds also proved that both the extracts are able to prevent mitosis. The extrapolation of these results can find opportunities in therapeutic regiments of cancer.

show abstract

“…have a broad range of medicinal properties, such as antimicrobial, anti-mutagenic, anti-HIV, immunopotentiating and antitumor activity [ 15 – 20 ] with numerous isolated bioactive compounds such as, vitamin C, vitamin E and β-carotene that can be harnessed for commercial use [ 21 ]. In our previous study, we reported that E. tuba possess potent in vitro antioxidant and in vivo iron chelation activity [ 22 , 23 ] and numerous phytochemicals such as phenolics, flavonoids, alkaloids, tannins, terpenoids, triterpenoids, saponin, glycoside and carbohydrates are present in adequate amount in the extract which was confirmed by phytochemical analysis [ 22 ]. In this study, we first demonstrated the antiproliferative effect of 70 % methanolic extract of E. tuba (ETME) on lung and breast cancer cells and normal fibroblast cells in vitro.…”

Section: Introductionmentioning

confidence: 90%

A microalga, Euglena tuba induces apoptosis and suppresses metastasis in human lung and breast carcinoma cells through ROS-mediated regulation of MAPKs

2016

Self Cite

View full text Add to dashboard Cite

BackgroundEuglena tuba, a microalga, is known for its excellent antioxidant and iron-chelation activities; however its anticancer efficacies have not been reported yet. This study investigates the antitumor and antimetastatic activities of 70 % methanolic extract of Euglena tuba (ETME) against human lung (A549) and breast cancer (MCF-7) cells in vitro. Moreover, we had examined ETME’s role in inducing intracellular ROS with the regulation of antioxidants and MAPK pathway.MethodsAnticancer activity of ETME was thoroughly studied using flow cytometry, confocal microscopy and western blotting; along with various biochemical assays for analysing ROS-induced regulation of antioxidant enzymes. Inhibition of invasion and migration of malignant cells by ETME were investigated by wound healing and zymographic studies. DNA–Protein interaction with ETME was also studied.ResultsETME inhibited the growth of both A549 (IC50 92.14 µg/ml) and MCF-7 cells (IC50 50.27 µg/ml) by inducing apoptosis, while remained non-toxic against nomral WI-38 cells (IC50 911.43 µg/ml). ETME treatment resulted in increasing Bax/Bcl-2 ratio, BID truncation and activation of caspase cascade. This ultimately leads to PARP degradation and apoptosis through the intrinsic and extrinsic pathway in both A549 and MCF-7 cells. Wound healing and gelatin zymography studies revealed that ETME significantly inhibited the invasion and migration of both A549 and MCF-7 cells dose-dependently through the downregulation of MMP-9. Further investigations showed that ETME selectively induces intracellular ROS, regulated the levels of intracellular antioxidants and suppresses the activation of ERK1/2, JNK, P38 mitogen-activated protein kinase pathways in both type of malignant cells. Further DNA and protein binding studies revealed that ETME strongly interact with DNA as well as protein attributing the possibilities of presence of components which are targeting the macromolecules in cancer cells. Moreover, when the identified compounds from ETME were examined for their cytotoxicities individually, it was found that they lost their specificities towards cancer cells and also attacked normal cells.ConclusionsOur study suggests that ETME retards the growth of both lung and breast cancer cells, in vitro, through multivariate mechanisms, proving its candidature for the development of better and safer drugs against these cancers.

show abstract

Assessment of the phytochemical constituents and antioxidant activity of a bloom forming microalgae Euglena tuba

Cited by 35 publications

References 34 publications

Carotenoids, Phenolic Compounds and Tocopherols Contribute to the Antioxidative Properties of Some Microalgae Species Grown on Industrial Wastewater

Carotenoids, Phenolic Compounds and Tocopherols Contribute to the Antioxidative Properties of Some Microalgae Species Grown on Industrial Wastewater

Antioxidant and antimitotic activities of sulfated polysaccharide from marine brown algae Padina tetrastromatica

A microalga, Euglena tuba induces apoptosis and suppresses metastasis in human lung and breast carcinoma cells through ROS-mediated regulation of MAPKs

Contact Info

Product

Resources

About