Antioxidant and antimitotic activities of sulfated polysaccharide from marine brown algae Padina tetrastromatica

Abstract

<p class="Default">Antioxidants play a central role in the prevention of carcinogenesis. The most natural compounds exhibit their protective effects by eliciting antioxidant potential. Sulfated polysaccharide was isolated from the brown algae <em>Padina tetrastromatica</em>, then purified and evaluated for its composition and <em>in vitro </em>antioxidant and antimitotic activities. Both ethanolic sulfated polysaccharide (ESPS) and ethanolic sulfated polysaccharide-column purified (ESPS-CP) exhibited considerable amount of carbohydrates (11.2% and 17.6%), sulfate (11.4% and 7.4%), fucose (5.5% and 15.7%), uronic acid (4.7% and 11.8%), xylose (0.5% and 0.03%) and sulfated polysaccharide (2.4% and 12.7%) content. The FTIR analysis and phytochemical screening also confirmed the presence of sulfated polysaccharides. In the <em>in vitro</em> antioxidant activity determination using DPPH (1-1-diphenyl 2-picryl hydrazyl) radical scavenging activity, hydroxyl radical scavenging activity, superoxide anion scavenging activity, hydrogen peroxide scavenging activity, total antioxidant activity and reducing power, ESPS showed more activity than ESPS-CP. In the case of nitric oxide radical scavenging, ESPS-CP was found to be more effective. At a concentration of 2mg/ml, both samples were potent antioxidants with significant IC<sub>50</sub> values. The antimitotic studies such as mitotic index in onion root tips and sprouting assay in green gram seeds also proved that both the extracts are able to prevent mitosis. The extrapolation of these results can find opportunities in therapeutic regiments of cancer.<strong></strong></p>

Attenuation of hydrogenperoxide-induced oxidative damages in L929 fibroblast cells by sulfated polysaccharides isolated from the edible marine algae Padina tetrastromatica

“…Results of the extraction and purification are already published. 7 SPS was found to contain appreciable percentage of total carbohydrates, sulfates, uronic acid, fucose, and xylose. 7…”

“…7 The chemical composition of SPS was already determined, 7 and it was well characterized using Fourier-transform infrared spectroscopy (FTIR), 7 nuclear magnetic resonance (NMR), 8 ultraviolet (UV)–visible spectrum and gel permeation chromatography. 9 SPS was proved to have antioxidant, 7 immunomodulatory, 8 anticancer, 9 and anti-proliferative 10 activities. This study presents high-performance liquid chromatography (HPLC) data and the potential of SPS to enhance intracellular antioxidant machinery in L929 mouse fibroblast cells during hydrogen peroxide (H 2 O 2 )-induced oxidative stress.…”

Evaluation of Pharmacological Properties of Caesalpinia bonducella Seed and Shell Extract

“…has been used in different system of traditional medication for the treatment of diseases and ailments of human beings. 26 Phytochemicals are a class of molecules found predominantly in tea, grapes, berries, cocoa and other plants.…”

A review on phytoconstituents of marine brown algae

“…Edible brown algae Eisenia arborea have phlorofucofuroeckol-B, shown strong antioxidant activity, it suggests a potential use in antiallergic drug preparations [44]. The sulfated polysaccharides from Padina tetrastomatica have been used in Hakim and Patel Future Journal of Pharmaceutical Sciences (2020) 6:129 cancer treatment because it showed strong antioxidant and anti-mitotic activity [87]. A brown algae Sargassum vestitum has active compound fucoxanthin which showed antioxidant activity, it suggests possible use in the food and pharmaceutical field [5].…”