Assessment of the gene content of the chromosomal regions flanking bovine DGAT1

Winter, Andreas; Alzinger, Ariane; Fries, R.

doi:10.1016/s0888-7543(03)00238-6

Cited by 19 publications

(10 citation statements)

References 23 publications

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“…Grisart et al (2002Grisart et al ( , 2004 and Winter et al (2002Winter et al ( , 2004 have identified a nonconservative dinucleotide substitution (K232A) in the acyl-CoA diacylglycerol acyltransferase1 (DGAT1) gene at position 10433 and 10434 in exon number 8 as the most likely mechanism underlying the QTL on this chromosome.…”

Section: Introductionmentioning

confidence: 99%

Effect of DGAT1 polymorphisms on the estimated breeding values of Czech Simmental sires

Hanusová¹,

Mikova²,

Večerek³

et al. 2014

CZECH J ANIM SCI

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ABSTRACT:The aim was to evaluate the effect of polymorphisms in the promoter and in the coding region of the DGAT1 gene on the estimated breeding values (EBV) of Czech Simmental sires. The K232A polymorphism (n = 191) in the coding region was genotyped by PCR/RFLP, and the KU and SA polymorphisms in the promoter (n = 203) were identified in an automatic sequencer. In the K232A polymorphism, the frequency of the genotype AA (Alanine) was greater than that of the genotype KA, the homozygous genotype KK (Lysine) was not found. Similarly, the allele A predominated over the K allele (0.945 and 0.055). The EBV for milk performance have been assigned to the genotypes, and the associations quantified. For the AA genotype and A allele, positive association with EBV of milk yield and protein yield was found, and negative association with the breeding values of fat percentage and yield, and protein percentage, but only the value of fat content was found to be significant. The positive non-significant association of the A variant with the protein yield was caused by the high milk yield. In the KU polymorphism, the CC genotype was associated significantly with lower EBV for the fat percentage, both the C allele and the CC genotype were associated with higher EBV for milk yield, so both the fat and the protein yield were non-significantly increased. For the diplotypes K232A/KU, there was a significant association with the fat percentage. The AACC combination seemed to have some breeding potential. The K232A polymorphism explained maximum of 6.2% of EBV variability, the KU polymorphism of 4.4%, and the SA polymorphism of 4.2%. The diplotypes K232A/KU explained maximum of 7.4% of variability. The highest proportion of variability was explained for fat percentage. The results confirmed the important role of the BTA14 region in controlling milk performance.

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Section: Introductionmentioning

confidence: 99%

Effect of DGAT1 polymorphisms on the estimated breeding values of Czech Simmental sires

Hanusová¹,

Mikova²,

Večerek³

et al. 2014

CZECH J ANIM SCI

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“…Currently, successful positional cloning studies using detailed contig maps of specific cattle genome regions have been rare, e.g. the identification of the bovine LIMBIN gene causing dwarfism in Japanese brown cattle (Takeda et al, 2002; BAC contig of BTA 1 195 Takeda and Sugimoto, 2003) or the analysis of the bovine DGAT1 gene as a functional candidate for milk yield and composition (Grisat et al, 2002(Grisat et al, , 2004Winter et al, 2002Winter et al, , 2004.…”

Section: Introductionmentioning

confidence: 99%

A 4 Mb high resolution BAC contig on bovine chromosome 1q12 and comparative analysis with human chromosome 21q22

et al. 2005

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The bovine RPCI-42 BAC library was screened to construct a sequence-ready ~4 Mb single contig of 92 BAC clones on BTA 1q12. The contig covers the region between the genes KRTAP8P1 and CLIC6. This genomic segment in cattle is of special interest as it contains the dominant gene responsible for the hornless or polled phenotype in cattle. The construction of the BAC contig was initiated by screening the bovine BAC library with heterologous cDNA probes derived from 12 human genes of the syntenic region on HSA 21q22. Contig building was facilitated by BAC end sequencing and chromosome walking. During the construction of the contig, 165 BAC end sequences and 109 single-copy STS markers were generated. For comparative mapping of 25 HSA 21q22 genes, genomic PCR primers were designed from bovine EST sequences and the gene-associated STSs mapped on the contig. Furthermore, bovine BAC end sequence comparisons against the human genome sequence revealed significant matches to HSA 21q22 and allowed the in silico mapping of two new genes in cattle. In total, 31 orthologues of human genes located on HSA 21q22 were directly mapped within the bovine BAC contig, of which 16 genes have been cloned and mapped for the first time in cattle. In contrast to the existing comparative bovine–human RH maps of this region, these results provide a better alignment and reveal a completely conserved gene order in this 4 Mb segment between cattle, human and mouse. The mapping of known polled linked BTA 1q12 microsatellite markers allowed the integration of the physical contig map with existing linkage maps of this region and also determined the exact order of these markers for the first time. Our physical map and transcript map may be useful for positional cloning of the putative polled gene in cattle. The nucleotide sequence data reported in this paper have been submitted to EMBL and have been assigned Accession Numbers AJ698510–AJ698674.

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“…Two SNPs in DGAT1 gene associated the most with CHL_milk (Table 2), milk yield (Table 3) and fat yield ( Table 4). The DGAT1 gene, located in the centromeric region of bovine chromosome 14, harbors a quantitative trait nucleotide for milk yield and milk component traits (milk fat yield, percentage fat, protein yield and percentage protein) (Boichard et al 2003;Bennewitz et al 2004;Grisart et al 2004;Winter et al 2004;Jiang et al 2010;Wang et al 2012) and it is also known as an important gene in the regulation of cholesterol metabolism (Yamazaki et al 2005;Chandak et al 2011;Sachdev et al 2016). The significant associations of rs523413537 and rs109326954 in DGAT1 gene with CHL_milk, milk yield and fat yield support the important role of this gene for milk yield and milk related traits in bovine.…”

Section: Discussionmentioning

confidence: 99%

Targeted genotyping to identify potential functional variants associated with cholesterol content in bovine milk

et al. 2020

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Summary High blood cholesterol concentration, mainly caused by high dietary cholesterol, is a potential risk factor for human health. Dairy products are important sources of human dietary cholesterol intake. Therefore, monitoring bovine milk cholesterol concentration is important for human health benefit. Genetic selection for improvement of cow milk cholesterol content requires understanding of the genetics of milk cholesterol. For this purpose, we performed analyses of additive and dominance effects of 126 potentially functional SNPs within 43 candidate genes with milk cholesterol content [expressed as mg of cholesterol in 100 g of fat (CHL_fat) or in 100 mg of milk (CHL_milk)]. The additive and dominance effects of SNPs rs380643365 in AGPAT1 (P = 0.04) and rs134357240 in SOAT1 (P = 0.035) genes associated significantly with CHL_fat. Moreover, five (rs109326954 and rs523413537 in DGAT1, rs109376747 in LDLR, rs42781651 in FAM198B and rs109967779 in ACAT2) and four (rs137347384 in RBM19, rs109376747 in LDLR, rs42016945 in PPARG and rs110862179 in SCAP) SNPs were significantly associated with CHL_milk (P < 0.05) based on additive and dominance effect analyses respectively. Rs109326954 and rs523413537 in DGAT1 explained a considerable portion of the phenotypic variance of CHL_milk (7.54 and 6.84% respectively), and might be useful in selection programs for reduced milk cholesterol content. Several significantly associated SNPs were in genes (such as ACAT2 and LDLR) involved in cholesterol metabolism in the liver or cholesterol transport, suggesting multiple mechanisms regulating milk cholesterol content. Nine and seven SNPs identified by additive or dominance effect analyses associated significantly with milk yield and fat yield respectively. Further analyses are required to better understand the consequences of these variants and their potential use in genomic selection of the studied traits.

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Assessment of the gene content of the chromosomal regions flanking bovine DGAT1

Cited by 19 publications

References 23 publications

Effect of DGAT1 polymorphisms on the estimated breeding values of Czech Simmental sires

Effect of DGAT1 polymorphisms on the estimated breeding values of Czech Simmental sires

A 4 Mb high resolution BAC contig on bovine chromosome 1q12 and comparative analysis with human chromosome 21q22

Targeted genotyping to identify potential functional variants associated with cholesterol content in bovine milk

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