Assessment of the effect of α-galactosides injected during embryogenesis on selected chicken traits

Abstract

Assessment of the effect of "-galactosides injected during embryogenesis on selected chicken traits. Folia biol. (Kraków) 53: 13-20. The effect of different doses of "-galactoside (RFOs) preparations from Pisum sativum L. cv. Opal, injected into eggs during embryogenesis, on maintaining a high number of bifidobacteria, selected chicken broiler traits and the lipoprotein level of blood were studied. Two independent experiments were conducted. In the first, Ringer water solution containing 1.763 mg/egg of fructooligosaccharides (FOS) (I group), 2.1158 mg of pea RFO preparation containing 20% sucrose (II group) and 0.4232 mg of sucrose (III group) were injected into Hubbard broiler breeder eggs containing 12-day old embryos. Only Ringer water solution was applied to the eggs of the control group (IV group). The number of bifidobacteria determined in faeces of two-day old chicken of groups I and II was significantly higher in comparison with the sucrose and control groups. The high level of bifidobacteria of groups I and II was maintained during 6 weeks. The dose of both preparations had no influence on the body weight, carcass, breast muscle, leg and abdominal fat ratio, total cholesterol, HDL and LDL serum concentrations. Broiler mortality and breast muscle cholesterol concentration was highest (P<0.05) for the control group. On the other hand, the European Production Index, as well as serum triglycerides, were the lowest for this group. The second experiment was performed on Hybro G chicken breeder eggs. 0.69, 3.43 and 6.87 mg/egg of pea RFO preparation doses containing 20% sucrose were injected into the experimental groups. The number of bifidobacteria in the caecum and selected meat traits of broilers were determined. The results of this experiment confirmed that RFO injection in ovo causes the long-time maintenance of a high level of bifidobacteria. The dose of the preparations does not have any effect on the selected broiler meat traits, except that the highest dose increases the percent of carcase in body weight. However, this dose reduced the hatchability of the treated embryos.

Influence of different prebiotics and mode of their administration on broiler chicken performance

“…In Trial 1, both prebiotics injected in ovo (DN and BI) stimulated number of lactobacilli and bifidobacteria in chicken feces. This observation confirmed our earlier findings, that a single in ovo prebiotic (oligosaccharides) injection into 12-day-old chicken embryo leads to an increased number of bifidobacteria at the moment of hatching; it also ensures the long-term maintenance of a high level of intestinal bifidobacteria (Villaluenga et al, 2004;Pilarski et al, 2005). The mechanism of this process is of considerable interest, particularly in the light of the commonly held opinion that embryonic development of the chicken happens in a sterile environment (Amit-Romach et al, 2004).…”

“…Based on chemical and physical features and dose of the injected substance, different site of injection (i.e. the embryo, the amnion, the allantois, the air cell or the yolk sac) and embryo age (0, 12, 17 or 18 days of incubation) have been used (Casas-Perez and Edens, 1995;Pilarski et al, 2005;Uni et al, 2005;Cheled-Shoval et al, 2011;Ebrahimnezhad et al, 2011).…”

“…Third, prebiotics should confer beneficial properties to the host in performance and fitness traits. So far, our 'golden standard' for in ovo injection was raffinose family oligosaccharides (RFO) extracted from lupin, which assures long-term maintenance of a high level of intestinal bifidobacteria at hatching (Villaluenga et al, 2004;Pilarski et al, 2005) and optimal performance of broiler chickens (Bednarczyk et al, 2011). However, there are many other biologically active oligosaccharides available, that could be validated for in ovo injection.…”

Effects of the Replacement of Soybean Meal with Pea as Dietary Protein Source on the Serum Protein Fractions of Broilers

“…Spielmann et al (2008) observed that pea protein has a low methionine content and a high arginine content. The addition of pea meal as a source of protein into broiler diets can improve carcass quality and may result in favorable lipid profile, without adversely affecting growth performance traits (Igbasan & Guenter, 1996;Pilarski et al, 2005;Laudadio et al, 2012). The broiler serum proteins and fractions can be affected by the application of different feedstaff (Bingol et al, 2016).…”

Studies on the Influence of Different Nitrogen Forms on the Chemical Composition of Various Cultivars ofLupinus albusL.

“…However, the opinion about harmfulness of RFOs has been recently negated. It is thought that these sugars belong to the class of favorable natural prebiotics (Gulewicz et al 2002;MartinezVillaluenga et al 2004;Pilarski et al 2005). On the basis of these results, discussion on influence of different N form on level of RFOs seems to be complicated.…”