Assessment of the cytotoxic, genotoxic, and antigenotoxic potential of Pycnogenol® in in vitro mammalian cells

Taner, Gökçe; Aydın, Sevtap; Aytaç, Zekí; Başaran, Arif Ahmet; Başaran, Nurşen

doi:10.1016/j.fct.2013.06.053

Cited by 20 publications

(18 citation statements)

References 62 publications

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“…In this study, PYC has not showed cytotoxic effects at the concentrations of up to 150 μg/mL in CHO cells during 24 h exposure but above this concentration the cytotoxicity of PYC has started and the cell viability was decreased below 50% at 300 μg/mL. 27 There is limited study about cytotoxicity of puearin. In a single study, it is demonstrated that puerarin has shown cytotoxic effects on HT-29 cells in a dose and time dependent manner.…”

Section: Discussionmentioning

confidence: 99%

“…The effects of curcumin on the viability of human leukemia cell lines (U937 and Molt4) by MTT assay were also determined and dose dependent cytotoxic effects of curcumin were found. 26 Taner et al 27 demonstrated the cytotoxic profile of PYC in healthy CHO cells. In this study, PYC has not showed cytotoxic effects at the concentrations of up to 150 μg/mL in CHO cells during 24 h exposure but above this concentration the cytotoxicity of PYC has started and the cell viability was decreased below 50% at 300 μg/mL.…”

Section: Discussionmentioning

confidence: 99%

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Assessment of Cytotoxicity Profiles of Different Phytochemicals: Comparison of Neutral Red and MTT Assays in Different Cells in Different Time Periods

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Başaran

et al. 2017

tjps

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ÖZAmaç: Fenolik bileşikler sağlığı koruyucu farklı özellikler gösterir. Galangin, kurkumin, piknogenol, puerarin ve ursolik asit halk tıbbında yaygın olarak kullanılan bitkisel fenoliklerdendir. Bu çalışmanın amacı, nötral kırmızı alım (NKA) ve MTT yöntemleri arasındaki farkı sağlıklı hücreler ve kanser hücrelerinde farklı bitkisel fenoliklerin (galangin, kurkumin, piknogenol, puerarin ve ursolik asit) farklı zaman aralıklarında farklı zaman aralıklarında belirlemektir. Gereç ve Yöntemler: Bu çalışmada, bu fenolik bileşiklerin sitotoksik etkileri sağlıklı hücreler (Çin hamster fibroblast hücre hattı, V79) ve kanser [insan serviks epitelyal adenokarsinoma hücre hattı, Henrietta Lacks (HeLa) ve insan meme karsinoma hücre hattı (BT-474)] hücrelerinde 18, 24 ve 48 saatlik inkübasyon sürelerinde NKA ve MTT yöntemleriyle değerlendirilmiştir. Bulgular: Bulgularımız galangin, kurkumin, piknogenol, puerarin ve ursolik asitin V79, HeLa ve BT-474 hücre canlılıklarını 18, 24 ve 48 saatlik inkübasyon sürelerinde doza bağımlı olarak azalttığını göstermiştir. Ancak en az hücre canlılık oranı 48 saatlik inkübasyon sonrası görülmüştür. NKA ile MTT yöntemlerinin sonuçları arasında fark görülmemiştir. Sonuç: Sitotoksisite analizinde kullanılacak yöntem ve inkübasyon süresinin belirlenmesi için maddelerin sitotoksisite mekanizması bilinmelidir. Anahtar kelimeler: MTT, nötral kırmızı, bitkisel fenoliklerObjectives: Phenolic compounds exhibit several health protective properties. Galangin, curcumin, pycnogenol, puerarin and ursolic acid are commonly used plant phenolics in folk medicine. The aim of our study was to evaluate the difference between neutral red uptake (NRU) and MTT assays using different plant phenolics (galangin, curcumin, pycnogenol, puerarin and ursolic acid) in healthy and cancer cells in different time periods. Materials and Methods: In this study, the cytotoxic effects of these phenolic compounds were investigated by NRU and MTT assays in healthy (V79, Chinese hamster fibroblast cell line) and cancer [human cervix epithelial adenocarcinoma cell line Henrietta Lacks (HeLa) and human mammary carcinoma cell line (BT-474)] in 18, 24 and 48 h incubation periods. Results: Our results demonstrated that galangin, curcumin, pycnogenol, puerarin and ursolic acid decreased cell viability of V79, HeLa and BT-474 cells in a dose-dependent manner in 18, 24 and 48 h incubation periods. However, the cell survival rate was much lower in 48 h incubation period. There was no difference between the results from NRU and MTT assays. Conclusion: To decide which incubation period and which cytotoxicity study to be used, the cytotoxicity mechanism of the compound must be known.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Assessment of Cytotoxicity Profiles of Different Phytochemicals: Comparison of Neutral Red and MTT Assays in Different Cells in Different Time Periods

Bacanlı

Anlara

Başaran

et al. 2017

tjps

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“…Pycnogenol has been used as a herbal dietary supplement in European countries because of its anti-oxidant effect (8). Recent studies have demonstrated its potential anti-inflammatory activity in several chronic diseases (8,15).…”

Section: Discussionmentioning

confidence: 99%

“…Recent studies have demonstrated its potential anti-inflammatory activity in several chronic diseases (8,15). It was reported that PYC is a hepatoprotective agent when administered at a dose of 10 mg/kg in a model of nonalcoholic fatty liver disease caused by oxidative stress (16).…”

Section: Discussionmentioning

confidence: 99%

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Inhibitory Effect of Pycnogenol® on Airway Inflammation in Ovalbumin-Induced Allergic Rhinitis

Günel¹,

Demirci²,

Eryılmaz³

et al. 2016

Balkan Med J

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Antiproliferative and immunostimulatory activity of a protein from Pleurotus eryngii

et al. 2014

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Assessment of the cytotoxic, genotoxic, and antigenotoxic potential of Pycnogenol® in in vitro mammalian cells

Cited by 20 publications

References 62 publications

Assessment of Cytotoxicity Profiles of Different Phytochemicals: Comparison of Neutral Red and MTT Assays in Different Cells in Different Time Periods

Assessment of Cytotoxicity Profiles of Different Phytochemicals: Comparison of Neutral Red and MTT Assays in Different Cells in Different Time Periods

Inhibitory Effect of Pycnogenol® on Airway Inflammation in Ovalbumin-Induced Allergic Rhinitis

Antiproliferative and immunostimulatory activity of a protein from Pleurotus eryngii

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