Assessment of the biological activity of fish muscle protein hydrolysates using in vitro model systems

Blue Whiting Protein Hydrolysates Exhibit Antioxidant and Immunomodulatory Activities in Stimulated Murine RAW264.7 Cells

“…Similar to the BWSPHs tested in this study, which contained a high content of low MW peptides, large yellow croaker (Pseudosciaena crocea) protein hydrolysate (MW < 3 kDa) exhibited O 2 − • scavenging activity and DPPH scavenging activity in vitro and also effectively and dose-dependently (50-300 µg/mL) increased the activity of antioxidant enzymes GSH-Px, SOD, and CAT in H 2 O 2 -induced oxidative stress in HepG2 cells (p < 0.05) [3]. In addition, numerous fish-derived peptides have demonstrated ability to modulate oxidative stress pathways in vitro [4,5,9,47,48].…”

“…A number of antioxidative fish protein hydrolysates with potential to induce health benefits via promoting cellular redox balance have been identified [3][4][5][6][7][8]. However, the generation of antioxidant protein hydrolysates is dependent on hydrolysis conditions (protease source, temperature, pH, and degree of hydrolysis (DH)), which ultimately influences the peptide profile and amino acid composition of the resulting fractions [9]. Small molecular weight (MW) fractions rich in hydrophobic amino acids and hydrophobic diand tripeptides are generally reported to exhibit effective antioxidant activity owing to the proton donating or electron/lipid radical scavenging ability of hydrophobic amino acids.…”

Muscle Protein Hydrolysates and Amino Acid Composition in Fish

“…Fish muscle protein contains a well-balanced amino acid composition; specifically, Lys accounts for 8.8%, Trp 1.0%, His 2.0%, Phe 3.9%, Leu 8.4%, Ile 6.0%, Thr 4.6%, Met-Cys 4.0%, and Val 6.0% [ 18 ]. The consumption of fish muscle protein is associated with health benefits, particularly anti-inflammatory, antioxidant, and angiotensin-converting enzyme inhibitory activities and antimicrobial effects [ 10 , 19 , 20 , 21 , 22 , 23 ]. Hydrolysates of fish muscle proteins with good nutritional composition, amino acid profiles, and biological activities are easily absorbed in the gastrointestinal tract, and their bioavailability and stability due to decreased fragments have attracted attention in terms of producing valuable food ingredients.…”

“…The muscle proteins have abundant essential amino acids, such as lysine, tryptophan, histidine, phenylalanine, leucine, isoleucine, threonine, and methionine–cystine, accounting for 9.3%, 1.0%, 2.1%, 4.2%, 8.9%, 6.4%, 4.9%, and 4.2% of the total protein content, respectively [ 9 ]. Because of the high demand for better pharmaceutical and nutritional products, interest in high-quality nutritional components and in the various bioactivities of the hydrolysate of fish muscle protein with high sensitivity to proteolytic breakdown is increasing [ 10 ]. Therefore, efforts have been directed toward full absorption of essential nutrients and amino acids present in fish by hydrolyzing fish proteins.…”

Threadfin bream surimi gel containing squid fin protein hydrolysate: Textural properties, acceptability, and volatile profile

“…PH are known to be rich in peptides and nutrients (Jemil et al., 2014). The bioactive peptides in PH had pronounced metal chelating and free radicals scavenging activities, in which oxidative damage toward food proteins/lipids could be limited (Heffernan et al., 2021). Moreover, antioxidative peptides were able to lower oxidative stress, associated with free radicals generated in the human body, which cause several diseases, such as aging, hypertension, cancer, and so forth (Heffernan et al., 2021; Taroncher et al., 2021).…”