Assessment of the antibacterial activity of phenylethanoid glycosides from Phlomis lanceolata against multiple-drug-resistant strains of Staphylococcus aureus

Abstract

Three phenylethanoid glycosides, forsythoside B (1), phlinoside C (2) and verbascoside (3), were isolated from the methanol extract of the leaves of Phlomis lanceolata, an Iranian medicinal plant, by reversed-phase preparative high-performance liquid chromatography (HPLC), and the structures of these compounds were elucidated conclusively by ultraviolet (UV), mass spectrometry (MS) and a series of 1D and 2D nuclear magnetic resonance (NMR) analyses. The antibacterial properties of 1-3 against five multi-drug-resistant (MDR) strains of Staphylococcus aureus have been assessed by the rapid and robust microtitre-plate-based serial dilution method. While compounds 1 and 3 showed considerable activities against all five strains, compound 2 was inactive at the test concentrations.

Phytochemistry and bioactivity of Pedicularis sibthorpii growing in Iran

“…UV, 1 H NMR (200 MHz, CD 3 OD) and 13 C NMR (50 MHz, CD 3 OD) data were in agreement with the published data (Nazemiyeh et al, 2008c;Delazar et al, 2005Delazar et al, , 2012.…”

“…Solid-phase extraction (SPE) of the MeOH extract of the aerial parts of Pedicularis sibthorpii Boiss., Scrophulariaceae, followed by reversed-phase prep-HPLC analyses of the SPE fractions (10, 20, 40 and 60% aqueous MeOH fractions) afforded three phenylethanoids, verbascoside (1, Nazemiyeh et al, 2008c;Delazar et al, 2005, martynoside (2, Toth et al, 2007) and isomartynoside (3, Calis et al, 1984), an iridoid, aucubin (4, Bernini et al, 1984;Rønsted et al, 2000), a flavonoid glycoside, luteolin 7-O-β-D-glucopyranoside (5, (Mabry et al, 1970;Suntar et al, 2012), and a monosaccharide mannitol (6, Bock & Pedersen, 1983;SDBS, 2012). All isolated compounds were identified unequivocally by UV and NMR analyses.…”

Essential oil composition and isolation of freeradical-scavenging phenolic glycosides from the aerial parts of Ajuga chamaepitys growing in Iran

“…Isolated glycosides were identified unequivocally as the phenylethanoid glycoside, acteoside (1; Delazar et al, 2005;Nazemiyeh et al, 2008c), and two flavone glycosides, chrysoeriol 7-O-glucopyranoside (2; Mabry et al, 1970;Wagner et al, 1976;Wenkert & Gottlieb, 1977;Zhang & Li, 2008;Zhao et al, 2009;Zhou et al, 2009) and apigenin 7-O-rhamnopyranoside (3; Mabry et al, 1970;Wagner et al, 1976;Wenkert & Gottlieb, 1977;Iwashina & Matsumoto, 1994;Chunsriimyatav et al, 2009), by extensive UV spectroscopic analyses using various shift reagents (Mabry et al, 1970) and NMR analyses (Tables 1 and 2). All spectroscopic data were comparable with respective published data.…”

Phenylethanoid Glycosides: Research Advances in Their Phytochemistry, Pharmacological Activity and Pharmacokinetics

“…Compounds 121 and 125 showed considerable antibacterial activities against all strains of S. aureus with the minimum inhibitory concentration (MIC) values ranging from 64 µg/L to 256 µg/L. Particularly, the activities of 121 (MIC = 2.1ˆ10´4 and 4.1ˆ10´4 M) and 125 (MIC = 3.4ˆ10´4 M) against SA 1199B (NorA) and XU 212 (TetK/MecA), respectively, were comparable to those of the positive control, norfloxacin (MIC = 1.0ˆ10´4 and 2.5ˆ10´5 M) [90]. In addition, 4 111 -O-acetylacteoside (133) and 121 possessed significant inhibition of the formation of bacterial biofilms by E. coli UTI89 [91].…”