Assessment of TET1 gene expression, DNA methylation and H3K27me3 level of its promoter region in eutopic endometrium of women with endometriosis and infertility

Adamczyk, Magdalena; Rawłuszko-Wieczorek, Agnieszka Anna; Wirstlein, Przemysław; Nowicki, Michał; Jagodzińśki, Paweł P.; Wender-Ożegowska, Ewa; Kędzia, Małgorzata

doi:10.1016/j.biopha.2022.112989

Cited by 11 publications

(5 citation statements)

References 44 publications

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“…Many studies suggest that the TET family plays a role in the endometriosis and endometrial cancer (Mahajan et al, 2020). One study investigated the TET1 gene expression in the endometrium of the group of infertile endometriosis patients, infertile non-endometriosis patients, and in the group of patients without any reproductive disturbances (Adamczyk et al, 2022a). The study showed a significant decrease in the expression of TET1 gene in the group of infertile endometriosis patients compared to fertile patients, however, the expression remained unchanged in idiopathic infertile patients.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, complement-coagulation cascades, morphine addiction pathway, and PI3K-Akt signalling pathway were determined to be differentially expressed in UI patients which reveals the complexity of UI diagnosis (Keleş et al, 2022). It is known that TET family genes are associated with DNA demethylation process and its decrease was noticed in infertile endometriosis patients (Adamczyk et al, 2022a). BCL2 and BAK1 genes are cell death regulators and sometimes irregular patterns of the expression of these genes could reveal some underlying issues, for example, cancer (Witek et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Assisted reproductive technology outcomes and gene expression in unexplained infertility patients

Vaigauskaitė-Mažeikienė,

Baušytė,

Valatkaitė

et al. 2023

Front. Cell Dev. Biol.

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Background: Unexplained infertility (UI) can be a frustrating and challenging diagnosis for doctors and couples as it can be difficult to understand why they are unable to conceive despite increasing diagnostic tools. Assisted reproductive technology (ART) procedures have been successfully applied to many couples aiming to overcome UI. However, they can be not only expensive but also require multiple cycles to achieve a successful pregnancy. The endometrium and the follicular fluid have been investigated as target tissues not only to determine the cause of UI but also to increase conception rates.Results: In this study, we analyzed the outcomes of ART in 223 UI couples and gene expression associated with DNA modification, cell death, immune response and senescence (TET1, TET2, BCL2, BAK1, HMGA2, IL-6, IL-8) in infertile women’s endometrium and follicular fluid. We found significant differences in women who successfully got pregnant compared to women unable to conceive depending on age, duration of infertility, number of retrieved oocytes, zygotes, transferred embryos. Further, the expression of genes BAK1 (pro-apoptotic), TET2 (associated with epigenetic DNA modification) and IL-6 (associated with immune responses) were significantly higher in the endometrium of women who successfully got pregnant.Conclusion: Younger parental age couples showed higher ART success rates, shorter duration of infertility, higher number of retrieved oocytes, zygotes and transferred embryos. The gene expression analysis revealed significant changes in the endometrium depending on genes associated with cell death and immune response which were upregulated in females with diagnosed unexplained infertility.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Assisted reproductive technology outcomes and gene expression in unexplained infertility patients

Vaigauskaitė-Mažeikienė,

Baušytė,

Valatkaitė

et al. 2023

Front. Cell Dev. Biol.

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“…Diseases associated with TET1 include Glioblastoma and Rett Syndrome and cervical cancer. The important paralog of this gene is TET2 (13)(14)(15)(16)(17). Figure 1(C) shows the location of this gene on the chromosome.…”

Section: Tet1 Genementioning

confidence: 99%

The expression and clinical value of LRP11, FUBP1 and TET1 in cervical cancer

Xiaojie Zhao,

Suhua Li,

Jiexin Wen

et al. 2023

Cell Mol Biol (Noisy-le-grand)

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“…In pathological conditions such as chronic or acute inflammation, this window narrows or shifts to preclude normal implantation of the embryo, resulting in infertility or loss of pregnancy (7,14). Approximately 18.6%-30.6% of patients undergoing in vitro fertilization and embryo transfer (IVF-ET) experience WOI translocation, resulting in impaired endometrial receptivity, considered one of the main causes of pregnancy failure when transferring high-quality embryos (15)(16)(17)(18)(19).…”

Section: Introductionmentioning

confidence: 99%

“…To date, there are numerous molecular mediators reported to be involved in the regulation of endometrial receptivity, including cytokines, lipids, adhesion molecules, and growth factors (such as cyclooxygenase-2, Krueppel-like factor 5, leukemia inhibitory factor, interleukins, insulin-like growth factor-binding proteins, wingless/integrated factor, prolactin (PRL), vascular endothelial growth factor, and homeobox A10) ( 8 – 13 ). In pathological conditions such as chronic or acute inflammation, this window narrows or shifts to preclude normal implantation of the embryo, resulting in infertility or loss of pregnancy ( 7 , 14 ). Approximately 18.6%-30.6% of patients undergoing in vitro fertilization and embryo transfer (IVF-ET) experience WOI translocation, resulting in impaired endometrial receptivity, considered one of the main causes of pregnancy failure when transferring high-quality embryos ( 15 – 19 ).…”

Section: Introductionmentioning

confidence: 99%

Endometrial proteomic profile of patients with repeated implantation failure

Yang,

Wang,

et al. 2023

Front. Endocrinol.

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IntroductionSuccessful embryo implantation, is the initiating step of pregnancy, relies on not only the high quality of the embryo but also the synergistic development of a healthy endometrium. Characterization and identification of biomarkers for the receptive endometrium is an effective method for increasing the probability of successful embryo implantation.MethodsEndometrial tissues from 22 women with a history of recurrent implantation failure (RIF) and 19 fertile controls were collected using biopsy catheters on 7-9 days after the peak of luteinizing hormone. Differentially expressed proteins (DEPs) were identified in six patients with RIF and six fertile controls using isobaric tag for relative and absolute quantitation (iTRAQ)-based proteomics analysis.ResultsTwo hundred and sixty-three DEPs, including proteins with multiple bioactivities, such as protein translation, mitochondrial function, oxidoreductase activity, fatty acid and amino acid metabolism, were identified from iTRAQ. Four potential biomarkers for receptive endometrium named tubulin polymerization-promoting protein family member 3 TPPP3, S100 Calcium Binding Protein A13 (S100A13), 17b-hydroxysteroid dehydrogenase 2 (HSD17B2), and alpha-2-glycoprotein 1, zinc binding (AZGP1) were further verified using ProteinSimple Wes and immunohistochemical staining in all included samples (n=22 for RIF and n=19 for controls). Of the four proteins, the protein levels of TPPP3 and HSD17B2 were significantly downregulated in the endometrium of patients with RIF.DiscussionPoor endometrial receptivity is considered the main reason for the decrease in pregnancy success rates in patients suffering from RIF. iTRAQ techniques based on isotope markers can identify and quantify low abundance proteomics, and may be suitable for identifying differentially expressed proteins in RIF. This study provides novel evidence that TPPP3 and HSD17B2 may be effective targets for the diagnosis and treatment of non-receptive endometrium and RIF.

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Assessment of TET1 gene expression, DNA methylation and H3K27me3 level of its promoter region in eutopic endometrium of women with endometriosis and infertility

Cited by 11 publications

References 44 publications

Assisted reproductive technology outcomes and gene expression in unexplained infertility patients

Assisted reproductive technology outcomes and gene expression in unexplained infertility patients

The expression and clinical value of LRP11, FUBP1 and TET1 in cervical cancer

Endometrial proteomic profile of patients with repeated implantation failure

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