Assessment of sodium thiosulfate neutralizing effect on micro-hardness of dentin treated with sodium hypochlorite

Abstract

Background This study aims to evaluate the ability of sodium thiosulfate (STS) to neutralize the adverse effect of sodium hypochlorite (NaOCl) on dentin micro-hardness. Methods Fifty single-rooted teeth were longitudinally sectioned. The samples divided into a control and four sample groups (n = 20). All the samples were immersed in different solutions as follows, Control: Normal saline for 15 min, G1and G2: 2.5% NaOCl for 15 min, G3: 2.5% NaOCl for 15 min, followed by 5% STS for 10 min, G4: Normal saline for 15 min followed by 5% STS for 10 min. All groups except G1 incubated for one week before the test. The micro-hardness of samples was measured. Data were analyzed using the Kruskal–Wallis test for pairwise comparisons. A p value < 0.05 was considered significant. Results All groups showed a significant decrease in the micro-hardness value compared with the control group. NaOCl for one week (G2) reduced the micro-hardness of dentine compared with samples, tested immediately after immersion in NaOCl (G1) (p < 0.05). NaOCl alone (G2) or treated with STS (G3) resulted in a significant decrease in micro-hardness compared with the STS group (G4) (p < 0.05). Conclusions STS as a neutralizing agent could not prevent the dentin micro-hardness downturn caused by NaOCl.

A triton X-100 assisted PMAxx-qPCR assay for rapid assessment of infectious African swine fever virus

“…7% (w/v) glycine was used to stop the reaction of glutaraldehyde ( Cheung and Brown, 1982 ). The neutralizer used for the NaClO and VK was 0.5% (w/v) sodium thiosulphate (Na 2 S 2 O 3 ; Olmez-Hanci et al, 2014 ; Sahebi et al, 2020 ). Finally, all the disinfected samples were centrifuged at 10,000 × g (4°C) for 5 min to get the supernatants prior to storage on ice until use.…”