Assessment of Salt Stress to Arabidopsis Based on the Detection of Hydrogen Peroxide Released by Leaves Using an Electrochemical Sensor

Abstract

Salt stress will have a serious inhibitory effect on various metabolic processes of plant cells, this will lead to the excessive accumulation of reactive oxygen species (ROS). Hydrogen peroxide (H2O2) is a type of ROS that can severely damage plant cells in large amounts. Existing methods for assessing the content of H2O2 released from leaves under salt stress will cause irreversible damage to plant leaves and are unable to detect H2O2 production in real time. In this study, on the strength of a series of physiological indicators to verify the occurrence of salt stress, an electrochemical sensor for the detection of H2O2 released from leaves under salt stress was constructed. The sensor was prepared by using multi-walled carbon nanotube-titanium carbide‒palladium (MWCNT-Ti3C2Tx-Pd) nanocomposite as substrate material and showed a linear response to H2O2 detection in the range 0.05–18 mM with a detection limit of 3.83 μM. Moreover, we measured the determination of H2O2 released from Arabidopsis leaves at different times of salt stress by the sensor, which was consistent with conventional method. This study demonstrates that electrochemical sensing is a desirable technology for the dynamic determination of H2O2 released by leaves and the assessment of salt stress to plants.

Recent Advances in Novel Compositions for Electrochemical Applications

“…In the study of salt stress on Arabidopsis, X. Du and co-workers utilized an electrochemical sensor to detect hydrogen peroxide released by plant cells [24]. The sensor was modified with a nanocomposite made of MWCNT-Ti 3 C 2 T x -Pd, which enhanced the signal of the electrode.…”

“…This collection of articles presents extensive research and discoveries that leverage various morphologies of electrochemical compositions, emphasizing their multifunctional properties, such as advanced redox properties [24][25][26][27][28][29][30][31]. The published articles range from the drug design and synthesis of hybrids of sterically hindered phenols and diaryl ureas [31] to sophisticated studies on the enhancement of arc erosion resistance in AgCuO electrical contact materials [30].…”

“…The published articles range from the drug design and synthesis of hybrids of sterically hindered phenols and diaryl ureas [31] to sophisticated studies on the enhancement of arc erosion resistance in AgCuO electrical contact materials [30]. Other notable topics include in-depth research on chemical and electrochemical reductions of monoiminoacenaphthenes [29], investigations on conductive mediators based on ferrocene functionalized phosphonium ionic liquids [26], and critical assessments of salt stress based on innovative electrochemical sensor detection methodologies [24].…”

Assessment of Oxidative Stress by Detection of H2O2 in Rye Samples Using a CuO- and Co3O4-Nanostructure-Based Electrochemical Sensor

“…Abiotic stress factors, like high temperatures [8], drought [9], salinity [10], exposure to herbicides, and heavy metals [11], can disrupt the delicate equilibrium between ROS production and ROS scavenging in plants [12]. This can result in an accumulation of ROS, which can lead to oxidative damage to cellular components and impaired plant growth and development.…”

“…Electrochemical sensors demonstrate high sensitivity and are capable of detecting low H 2 O 2 concentrations in the nanomolar or micromolar range [33,34]. Electrochemical methods allow for the real-time, non-destructive measurement of H 2 O 2 levels in plant tissues [10]. Electrochemical methods exhibit high selectivity for H 2 O 2 , enabling researchers to measure H 2 O 2 concentrations in the presence of other ROS or interfering compounds.…”