Assessment of Rational Veterinary Drugs Use in Livestock at Adama District Veterinary Clinic, Central Ethiopia

Beyene, Takele; Assefa, Sultan; Ayana, Dinka; Jibat, Tariku; Tadesse, Fanos; Nigussie, Dereje; Beyi, Ashenafi Feyisa

doi:10.4172/2157-7579.1000319

Cited by 23 publications

(33 citation statements)

References 17 publications

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“…Due to this not only indiscriminate use of antimicrobials is common but also sale and distribution of counterfeit antimicrobials and the sale of antimicrobials on the informal market and involvement of untrained persons in the profession are big challenges [ 9 ]. Concerning the classes of antimicrobials prescribed for veterinary use in Ethiopia, reports from Adama and Bishoftu areas of central Ethiopia shows that oxytetracyclines of various formulations are the most commonly prescribed antimicrobial followed by penicillin-streptomycin fixed combination, sulpha drugs (sulphadimidine and sulphametoxazole-trimethoprim fixed combination), procaine penicillin, penicillin + cloxacilin, chloramphenicol, neomycin sulphate (intra-mammary infusion) and gentamicin [ 10 , 11 ]. According to the above authors, almost all (100%) cases in district veterinary clinics receive antimicrobial therapy after they had been tentatively diagnosed.…”

Section: Introductionmentioning

confidence: 99%

Identification and antimicrobial susceptibility profile of Escherichia coli isolated from backyard chicken in and around ambo, Central Ethiopia

et al. 2019

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BackgroundEscherichia coli is bacteria that exist as commensal in the intestine of animals and humans, but pathogenic strains cause disease in chickens. The development of antimicrobial resistance in E. coli is one of major concern worldwide. A cross-sectional study was conducted from November, 2015 to April, 2016 in and around Ambo town on backyard chicken with the objectives of isolating E. coli from selected visceral organs, assessment of potential risk factor and determination of antimicrobial resistance pattern of the isolates.ResultsThe overall isolation rate of E. coli was 11.5% (80/694) [95% CI: 9.64–14.61] and 32.5% (62/191) [95% CI: 25.39–39.09] at organ and chicken level, respectively. E. coli isolation rate was 15.2% (29/191), 13.6% (27/191), 6.3% (12/191) and 10.7% (13/121) from spleen, liver, kidney and ovary samples, respectively. The multivariable logistic regression analysis revealed higher probability of E. coli isolation from adult (adjusted Odds ratio [aOR] =2.5, P = 0.013) than younger chickens, from clinically sick chickens (aOR = 3.0, P = 0.003) than apparently healthy. E. coli isolates were 100% susceptible to ciprofloxacin, norfloxacin and sulfamethoxazole-trimethoprim followed by 89–63.4% susceptibility to gentamicin, streptomycin, ceftazidime, nalidxic acid, nitrofurantoin, kanamycin, amikacin and chloramphenicol. Whereas, 100% resistance was observed against cloxacilin, cefotaxime and amoxicillin, whereas 92.7 and 46.3% were resistant to cefuroxime, and tetracycline, respectively. Multidrug resistant (MDR) was observed in 78.1% (64/82) of the isolates which exhibited 5 different MDR patterns to 7 antimicrobial classes.ConclusionsHigher isolation rate of E. coli was observed from visceral organs of chickens. Age and health status were predictors of E. coli isolation. Remarkable numbers of the isolates are resistant to different antimicrobials and multidrug resistant E coli isolates are widespread in the area.Electronic supplementary materialThe online version of this article (10.1186/s12917-019-1830-z) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Identification and antimicrobial susceptibility profile of Escherichia coli isolated from backyard chicken in and around ambo, Central Ethiopia

et al. 2019

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“…In food safety, the development of a new method for controlling antibiotic residues in milk is crucial. Of great concern is the antibacterial family of fluoroquinolones [9], which are extensively used in veterinary treatments of food-producing animals [10,11] leaving residues in milk and tissues. Since the established maximum residual limits of this family in milk should be below 100 µg·Kg − , it is required to develop inexpensive and sensitive analytical methods for routine analysis of food product for human consumption.…”

Section: Introductionmentioning

confidence: 99%

Graphene quantum dots for enhancement of fluorimetric detection coupled to capillary electrophoresis for detection of ofloxacin

et al. 2019

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A new CE method with fluorescence detection is reported on the determination of ofloxacin in milk samples using graphene quantum dots (GQDs) for sensitivity enhancement. Injection of GQDs prior the standards/sample is crucial to increase the antibiotic fluorescence response. Clean‐up and preconcentration steps allowed for a good linear correlation in a concentration range between 50 and 1000 ng/mL for the ofloxacin, detection and quantification limits being 10.7 and 35.5 ng/mL, respectively. Optimal CE conditions for the seven‐fluoroquinolone separation method were assessed in terms of buffer type, pH, and voltage. The selective interaction of GQDs with ofloxacin as model analyte was subsequently studied finding a significant sensitivity improvement; therefore, the analytes would be detected at low concentrations by means of a commercial CE device equipped with a multi‐wavelength photoluminescence detector. Due to the different maximum emission wavelengths of the target compounds and the limitations shown by the single‐wavelength photoluminescence detector coupled to the CE system, we demonstrated the usefulness of the GQD‐assisted sensitivity‐enhanced CE method to determine ofloxacin in milk samples. This work opens an interesting possibility of using GQDs in separation techniques combined with photoluminescence detectors for lowering sensitivity levels typically provided by the mere device.

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“…Zoonotic bacteria may also acquire the immunity to the antimicrobial drugs in the living animal, and be lately transferred to other ones and to the population through the food chain or by direct contact. Besides, they can reach appreciable concentrations in the environment and disturb the ecological equilibrium [2,3]. In order to avoid these negative effects, several practices have been suggested to producers to rationalize the use of antibiotics, such as the prescription of therapeutic doses only to ill or at risk animal until the desired clinical response and under the supervision of a veterinary, and enlarge the withdrawal period [4].…”

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confidence: 99%

Validation of a procedure to quantify oxolinic acid, danofloxacin, ciprofloxacin and enrofloxacin in selected meats by micellar liquid chromatography according to EU Commission Decision 2002/657/EC

et al. 2017

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The suitability of an analytical method to determine oxolinic acid, danofloxacin, ciprofloxacin and enrofloxacin in edible tissues, based on micellar liquid chromatography coupled with fluorescence detection, to be applied in chicken, turkey, duck, lamb, goat, rabbit and horse muscle, is described. The method was fully matrix-matched in-lab revalidated, for each antimicrobial drug and meat, following the guidelines of the EU Commission Decision 2002/657/EC. The permitted limits were the maximum residue limits stated by the EU Commission Regulation 37/2010. The results obtained for the studied validation parameters were in agreement with the guidelines: selectivity (the antibiotics were resolved), linearity (r > 0.995), limit of detection (0.004-0.02 mg/kg), limits of quantification (0.01-0.05 mg/kg), calibration range (up to 0.5 mg/kg), recovery (89.5-105.0%), precision (<8.3%), decision limit, detection capability, ruggedness, stability and application to incurred samples. The method was found to be able to provide reliable concentrations with low uncertainty within a large interval, including the maximum residue limits, and then was useful to find out prohibited contaminated samples. The method did not require to be adapted for these matrices, and then it maintained its interesting advantages: short-time, eco-friendly, safe, inexpensive, easy-to-conduct, minimal manipulation and useful for routine analysis.

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Assessment of Rational Veterinary Drugs Use in Livestock at Adama District Veterinary Clinic, Central Ethiopia

Cited by 23 publications

References 17 publications

Identification and antimicrobial susceptibility profile of Escherichia coli isolated from backyard chicken in and around ambo, Central Ethiopia

Identification and antimicrobial susceptibility profile of Escherichia coli isolated from backyard chicken in and around ambo, Central Ethiopia

Graphene quantum dots for enhancement of fluorimetric detection coupled to capillary electrophoresis for detection of ofloxacin

Validation of a procedure to quantify oxolinic acid, danofloxacin, ciprofloxacin and enrofloxacin in selected meats by micellar liquid chromatography according to EU Commission Decision 2002/657/EC

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