Assessment of potential interactions between phytase and glycosidase enzyme supplementation on nutrient digestibility in broilers

Abstract

The effects of microbial 3-phytase and glycosidase enzymes, and their interactions, on energy values and nutrient digestibility in diets rich in nonstarch polysaccharides (NSP) were studied in diets based on corn, wheat, or barley. Four diets were prepared with each cereal grain. One had no enzymes, a second had 500 units of phytase, a third had glycosidase enzyme, and a fourth had phytase and glycosidase. The glycosidases used were alpha-galactosidase (corn diet), xylanase (wheat), and beta-glucanase (barley). Glycosidase decreased intestinal viscosity, whereas phytase increased this parameter in corn diets. Phytase increased AME in corn diets, whereas beta-glucanase in barley diets improved AME and AMEn, and digestibility of dry matter, starch, beta-glucans, and lipid. Xylanase in wheat diets improved dry matter and starch digestibility. Phytase increased total phosphorus retention in all diets, and significant interactions between glycosidase enzymes and phytase were detected in wheat and barley diets. Phytase decreased phosphorus excretion in corn and barley diets, whereas alpha-galactosidase increased phosphorus excretion in corn diets. Phytase in corn diets and beta-glucanase in barley diets increased calcium retention, whereas inclusion of xylanase decreased calcium retention in wheat diets. Phytase and beta-glucanase decreased calcium excretion in corn- and barley-based diets, respectively. An interaction was detected between phytase and beta-glucanase in barley diets, in which calcium excretion was reduced. In general, no negative interactions between phytase and glycosidase enzymes were found, indicating that both types of enzymes may be used together in feeds based on corn, wheat, or barley.

Evolving enzyme technology: impact on commercial poultry nutrition

“…So, in a diet to which both xylanase and phytase are added, what additivity in response can be expected, and will this be different in diets that are based on maize compared with those based on wheat? Recent work by Juanpere et al (2005) suggests that, as phytate is more closely associated with fibre in wheat than in maize, that the effects of xylanase on P retention and synergy (or at least additivity) with phytase will be more apparent in wheatbased diets. Data presented by Kim et al (2005) tend to support this, as they found that xylanase was capable of improving the retention of dietary P. Work by Cowieson & Adeola (2005) in maize-based diets demonstrated that a carbohydrase and protease cocktail could be successfully combined with phytase to additively enhance nutrient retention in a nutritionally marginal diet.…”

Xylanase in diets for growing pigs and broiler chicks

“…Diebold et al (2004) used xylanase at 5600 U kg -1 for pigs, Juanpere et al (2005) used 3540 U kg -1 of xylanase activities in wheat-based diet for broilers, the xylanase activity in the Kocher et al (2000) study was determined to be > 5000 U kg -1 of diet. Hence, it is important to investigate whether performance improvement can be observed at a lower inclusion level than reported above, and whether there is a supplementation level, above which further supplementation of the enzyme does not produce further improvement in performance.…”

The response of slow-growing chicks to the supplementations with different methionine levels and/or two types of enzymes

“…However, Wu et al (2004) concluded that phytase improved BWG and FCR by 17.5 and 2.9% respectively; meanwhile the corresponding improvements due to the addition of xylanase were 16.5 and 4.9% respectively. Juanpere et al (2005) reported that phytase increased intestinal viscosity and AME in corn diets.…”