Assessment of population genetic diversity of Derris elliptica (Fabaceae) in China using microsatellite markers

Li, He; Geng, Shilei

doi:10.1016/j.indcrop.2015.04.023

Cited by 8 publications

(7 citation statements)

References 34 publications

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“…First, if the weeds' expansion along highways was driven mainly by natural forces (presumably by wind, as this species produces typical achene-pappus units), then it would be restricted to short-distance (step-by-step) dispersal and would experience a series of population bottlenecks and genetic drift during colonization. Reductions in both allelic richness (AR) and genetic diversity would be expected for the roadside populations as the distance from Shenzheng (the first area invaded in mainland China by M. micrantha) increased (Hassel et al, 2005;Ma et al, 2011;Li and Geng, 2015). Second, if the expansion was facilitated by anthropogenic activity (vehicle transportation), then the weed would undergo long-distance dispersal.…”

Section: Introductionmentioning

confidence: 99%

Genetic variation in the invasive weedMikania micrantha(Asteraceae) suggests highways as corridors for its dispersal in southern China

Geng

Chen

Cai

et al. 2016

Ann Bot

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Background and Aims Roads as corridors of seed or fruit spatial dispersal have major impacts on the establishment and spread of invasive species, but their precise role in population genetic variation remains poorly understood. The South American weed Mikania micrantha has spread rapidly across southern China since its introduction to the Shenzhen area in 1984. This study investigated how its genetic diversity is distributed along highways, and whether highways have acted as corridors for the rapid expansion of M. micrantha.Methods Twenty-seven roadside populations were sampled along four highways in southern China, and 787 samples were examined using 12 microsatellite markers. Variation in genetic diversity among populations was quantified and patterns of genetic differentiation were analysed.Key Results A high level of genetic diversity was found at both the species and the population levels in this selfincompatible plant (expected heterozygosity ¼ 0Á497 and 0Á477, respectively; allelic richness ¼ 2Á580 and 2Á521, respectively). The Wright F-statistic value among populations (0Á044, P < 0Á01) and the analysis of molecular variance (91 % of genetic variation residing within populations, 9 % among populations within highways and 0 % among the four highways) showed a relatively low level of genetic differentiation among populations, while the principal coordinate and cluster analyses also indicated a lack of clear geographical genetic structure among populations. The calculated N m value of 5Á5 signifies strong gene flow.Conclusions The pattern of genetic variation is consistent with facilitated dispersal along highways. The genetic admixtures among the roadside populations imply the occurrence of multiple population introductions during colonization. The long-distance dispersal of seeds associated with vehicular transportation on highways may have played important roles in shaping the genetic variation. This finding highlights the importance of highways as corridors for the spread of M. micrantha in southern China.

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Section: Introductionmentioning

confidence: 99%

Genetic variation in the invasive weedMikania micrantha(Asteraceae) suggests highways as corridors for its dispersal in southern China

Geng

Chen

Cai

et al. 2016

Ann Bot

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“…Previous population studies (Wang et al, 2012;Zou, 2013;Chen, 2014;Yang et al, 2016;Lin, 2017;Jiang, 2018;Gao et al, 2022) all showed high genetic diversity of M. armatus populations in China, but the haplotype diversity in our result (hd=0.434) is lower than that in other population studies, for example, Wang et al (2012), Jiang (Jiang, 2018) and Gao et al (2022) reported the haplotype diversity in China was 0.965, 0.768 and 0.895, respectively. This may be due to different markers employed and the small sample size in our study compared to other studies.…”

Section: Discussionmentioning

confidence: 99%

“…So far, a lot of studies have been conducted on M. armatus, such as studies at individual level on their morphology (Shu et al, 2017;Zhou et al, 2019), nutritional composition (Wu et al, 2010;Fan et al, 2018), metal bioaccumulation (Javed and Usmani, 2016;Pandey et al, 2017), karyotype (Oliveira et al, 1997);, taxonomy (Jiang, 2018;Duong et al, 2020), reproduction (Serajuddin andPathak, 2012), histopathology (Dhole et al, 2011), sex-specific markers (Xue et al, 2020;Xue et al, 2021b), artificial breeding (Lin et al, 2016;Xue, 2018), Toll-Like Receptors (TLR) (Han et al, 2017;Han et al, 2019), mitochondrial genome sequencing (Li et al, 2016;Han et al, 2018), whole genome sequences at the chromosomal scale (Xue et al, 2021a), as well as population studies (Wang et al, 2012;Zou, 2013;Chen, 2014;Yang et al, 2016;Lin, 2017;Jiang, 2018;Thapliyal et al, 2020;Gao et al, 2022). Among the above listed population studies, some used mitochondrial DNA markers, such as COI (Chen, 2014;Jiang, 2018;Gao et al, 2022), Cytb (Wang et al, 2012;Thapliyal et al, 2020;Gao et al, 2022) and D-loop (Chen, 2014), and others employed nuclear makers, like SSR (Zou, 2013;Yang et al, 2016;Lin, 2017) and exon-pr...…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, we expanded our sampling area from China to Vietnam to fill our knowledge gap. Additionally, mitochondrial markers were mostly used in M. armatus population studies, only few nuclear markers like SSR and EPICs, while no reports were based on nuclear ribosomal DNA (nrDNA) markers to reveal their genetic diversity (Wang et al, 2012;Zou, 2013;Chen, 2014;Yang et al, 2016;Lin, 2017;Jiang, 2018;Gao et al, 2022). It is well known that nrDNA markers have been successfully employed in various fish population research (Mladineo et al, 2013;Garcia et al, 2015;Agdamar and Tarkan, 2019), such as 18S, ITS1, ITS2 and 28S, due to their high evolutionary rates (Presa et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

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Population genetics of zig-zag eel (Mastacembelus armatus) uncover gene flow between an isolated island and the mainland China

Yang

Wang²,

Wu³

et al. 2023

Front. Mar. Sci.

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IntroductionMastacembelus armatus is a commercially valuable fish, normally distributed in southern China and Southeast Asia. The natural population size of M. armatus is shrinking in recent years because of overfishing and habitat loss. In order to clarify the genetic diversity and differentiation of M. armatus populations, we collected 114 samples from eight populations in southern China and Vietnam and analyzed their population structure using nuclear ribosomal DNA sequences, the concatenated 18S and ITS2 regions.MethodsGenomic DNA from the fin clip was extracted and sequenced on an Illumina novaseq 6000 (Illumina, USA) high-throughput sequencing platform in accordance with the manufacturer’s instructions. After assembly and annotation, haplotype diversity, TCS network analysis, AMOVA analysis, population pairwise genetic distances, and UPGMA tree construction were conducted based on the concatenated sequences of 18S and ITS2.Results and discussionIn total, eleven nrDNA haplotypes were detected based on the concatenated sequences of 18S and ITS2. Amongst, three haplotypes were the main haplotypes, as representatives of three corresponding Clusters. There were two major Clusters in China, however, the Cluster in Vietnam was significantly divergent from the other two in China, likely due to the lack of river connection between China and Vietnam. Interestingly, based on low FST value, we found that gene flow occurred between the isolated island, Hainan Province, and the mainland China of Guangxi Province, probably as a result of exposed continental shelf connected them during glacial periods. In general, combing our data and literature data, genetic diversity and differentiation of M. armatus populations are relatively high regardless of spatial scale, although their natural population size is declining. This suggests that it is not too late to adopt measures to protect M. armatus, which benefits not only species itself but also the whole ecosystem.

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“…The thin band indicated the concentration of extracted DNA was less than 100 ng/μL. Such concentration is still enough for PCR that DNA template of 10-50 ng/μL was able to produce relatively stable amplification results (Guo and Gao 2017;Li and Geng 2015). In addition, SSR or microsatellite marker does not require extracted DNA with highconcentration, on the other hand, DNA polymerases that have high amplification efficiency are required for DNA with low concentration.…”

Section: Dna Extraction and Polymerase Chain Reactionmentioning

confidence: 99%

Fingerprinting sengon (Falcatria moluccana) accessions resistant to boktor pest and gall rust disease using microsatellite markers

2019

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Abstract. Siregar UJ, Rahmawati D, Damayanti A. 2019. Fingerprinting sengon (Falcatria moluccana) accessions resistant to boktor pest and gall rust disease using microsatellite markers. Biodiversitas 20: 2698-2706. Sengon (Falcataria moluccana Miq.) is a multipurpose fast-growing tree species and widely planted as community forest in Indonesia. According to Indonesian Light Wood Association (ILWA) F. moluccana wood products coming from forest industries in Jawa worth US$ 244.46 million export to China alone. The wood dust also has high potential as source for biomass-based energy in the form of wood-pellet. Monoculture plantation however often suffers from stem borer pest, known as boktor (Xystrocera festiva) and a gall rust disease, caused by fungi Uromycladium falcatarium. This research was aimed to characterize accessions of resistant and susceptible sengon individuals to both gall rust disease as well as stem borer pest using microsatellite markers. Totally 50 accessions of resistant and of resistant to stem borer pest were collected from Sumedang, West Jawa Province, while 88 accessions of resistant and of resistant to gall rust disease were sampled from Kediri, East Jawa Province and Sukabumi, Indonesia. Eight microsatellite markers could amplify most of the accessions used in this study and produce polymorphic fragments. High genetic diversity was detected in all of F. moluccana populations, with He ranged from 0.431 to 0.650. AMOVA showed that most genetic variations come from within populations. A dendrogram based on Nei’s genetic distance (1972) clustered some resistant accessions to either stem borer pest or gall rust disease separately from susceptible ones.

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Assessment of population genetic diversity of Derris elliptica (Fabaceae) in China using microsatellite markers

Cited by 8 publications

References 34 publications

Genetic variation in the invasive weedMikania micrantha(Asteraceae) suggests highways as corridors for its dispersal in southern China

Genetic variation in the invasive weedMikania micrantha(Asteraceae) suggests highways as corridors for its dispersal in southern China

Population genetics of zig-zag eel (Mastacembelus armatus) uncover gene flow between an isolated island and the mainland China

Fingerprinting sengon (Falcatria moluccana) accessions resistant to boktor pest and gall rust disease using microsatellite markers

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