Assessment of Pollen Viability in Guava Genotypes

Coser, Sara Morra; Fontes, Milene Miranda Praça; Ferreira, Márcia Flores da Silva

doi:10.17660/actahortic.2012.959.17

Cited by 8 publications

(7 citation statements)

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“…Pollen grains from the anthers of pea plants tested in vitro were excised and stained on glass slides, each with a drop of 1% carmine (Coser et al, 2012). They were covered with coverslips, and after a couple of minutes observed under the microscope (10 x lens).…”

Section: Pollen Viabilitymentioning

confidence: 99%

In vitro germination and viability of pea pollen grains after application of organic nano-fertilizers

et al. 2017

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The objective of this study was to evaluate the influence of two organic nanofertilizers, Lithovit and Nagro, on in vitro germination, pollen tube elongation and pollen grain viability of Pisum sativum L cv. Pleven 4. The effect of their application was high and exceeded data for the untreated control (44.2 and 47.23 % regarding pollen germination and pollen tube elongation, respectively), as well as the effect of the control organic algal fertilizer Biofa (17.5 and 27.9 %, respectively). Pollen grains were inoculated in four culture media. A medium containing 15% sucrose and 1% agar had the most stimulating impact on pea pollen grains. Pollen viability, evaluated by staining with 1% carmine, was within limits of 74.72-87.97%. The highest viability of pollen grains was demonstrated after the application of Nagro organic nano-fertlizer.

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Section: Pollen Viabilitymentioning

confidence: 99%

In vitro germination and viability of pea pollen grains after application of organic nano-fertilizers

et al. 2017

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“…The 22 guava tree genotypes exhibited large amount of viable pollen, with 16 of them presenting mean pollen viability of more than 84%, a value considered high in this study (Table 1); an overall mean of 93.46% was obtained in the three harvests. In wild guava trees, pollen viability values between 73% and 99% have been reported using Lugol and Acetic Orcein (Coser, et al, 2012a). However, the present study is the first account of this feature being tracked across the first harvests of an orchard in establishment process; this way, important information is provided for the crop, as crosspollination has been related as an important factor regarding productivity, with production losses occurring due to selffertilization (Alves and Freitas, 2007).…”

Section: Pollen Viabilitymentioning

confidence: 83%

Genetic parameters of pollen viability in guava (Psidium guajava L.)

Silva¹,

Silva²,

Marçal³

et al. 2017

Aust J Crop Sci

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Pollen viability is essential for the sexual reproduction in plants. Genetic and environmental factors as well as plant age can influence this characteristic. In this work, pollen viability was studied in guava (Psidium guajava L.), a cross-pollination species. The genetic parameters for this characteristic were estimated considering 22 genotypes, two environments and different plant ages. For that, the pollen viability of the genotypes was evaluated for three years (2013, 2014 and 2015) in two experimental orchards (installed in randomized block design, with three blocks and two plants per plot) and at two different regions of the state of Espírito Santo, Brazil. The plants were analyzed at 19, 24 and 38 months of age in the years 2013 and 2014 in Mimoso do Sul (ES) and 2015 in Linhares (ES), respectively. The flower buds, at pre-anthesis stage, were collected, fixed in ethanol:acetic acid (3:1) and stored at -20°C. Pollen viability was obtained by colorimetric methods (Alexander's, Acetic Orcein and Lugol dyes) and the genetic parameters estimated by means of mixed models. The pollen viability of the genotypes was high, with an overall mean of 93.46% in the thr ee harvests. Mean heritability was lower in the harvests of 2013 (0.479) and 2014 (0.126) in relation to 2015 (0.583), indicating a slighter possibility of predicting genetic gains based on this characteristic. Altogether, these results provide information about pollen viability in commercial and superior genotypes of the guava crop used in this study, given that pollen-donor genotypes are reported to influence characteristics related to weight and fruit quality in this species. In addition, these genotypes showed good potential for cross-pollination, and can therefore be used as pollinators in orchards and crosses within breeding programs.

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“…The results obtained in the present study are in agreement with that statement, given that our pollen germination data indicated a significantly lower rate than that observed when using staining materials. Similarly, when analysing pollen viability for guava genotypes (Psidium guajava), in which estimations had been made for in vitro pollen germination and staining method germination (Lugol's solution and acetic orcein), Coser et al (2012) observed that the results obtained through the use of staining materials were better than those when using in vitro methods. These authors state that the use of staining materials, while extremely attractive due to their simplicity and ease of use, overestimate guava pollen viability when compared with results obtained in vitro.…”

Section: Discussionmentioning

confidence: 98%

Morphology and viability of pollen grains from passion fruit species (Passiflora spp.)

et al. 2013

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The characterization and viability of pollen grains are useful tools to guide crosses in breeding programs. The objective of this study was to describe the morphological patterns and viability of pollen grains from five accessions of Passiflora edulis f. flavicarpa O. Deg. and five accessions of Passiflora setacea DC. Pollen morphology descriptions were made using light microscopy and scanning electron microscopy, whereas the viability analysis was performed by in vitro germination and histochemical analysis (Lugol's solution and 2,3,5-triphenyltetrazolium chloride). Pollen grains assessed for germination were inoculated in culture medium containing Ca(NO 3 ).4H 2 O (0.03%), Mg(SO 4 ).7H 2 O (0.02%), KNO 3 (0.01%), H 3 BO 3 (0.01%), sucrose (15%) and agar (0.8%). Although P. edulis and P. setacea showed the same shape and type of pollen aperture, the two differed in terms of their morphology and exine ornamentation pattern. In vitro analysis showed that one of the P. edulis f. flavicarpa accessions (designated BGP 330) presented the highest germination rate (53.98%) and longest pollen tube (2.18 mm). The histochemical analysis overestimated pollen viability when compared with the in vitro results. The results of this study contribute to the breeding of Passiflora species by increasing the understanding of their morphology and pollen grain viability.

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Assessment of Pollen Viability in Guava Genotypes

Cited by 8 publications

References 0 publications

In vitro germination and viability of pea pollen grains after application of organic nano-fertilizers

In vitro germination and viability of pea pollen grains after application of organic nano-fertilizers

Genetic parameters of pollen viability in guava (Psidium guajava L.)

Morphology and viability of pollen grains from passion fruit species (Passiflora spp.)

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