Assessment of periportal fibrosis in Schistosomiasis mansoni patients by proton nuclear magnetic resonance-based metabonomics models

Abstract

BACKGROUND The evaluation of periportal fibrosis (PPF) is essential for a prognostic assessment of patients with Schistosomiasis mansoni . The WHO Niamey Protocol defines patterns of fibrosis from abdominal ultrasonography, 1 H-nuclear magnetic resonance (NMR)-based metabonomics has been employed to assess liver fibrosis in some diseases. AIM To build 1 H-NMR-based metabonomics models (MM) to discriminate mild from significant periportal PPF and identify differences in the metabolite profiles. METHODS A prospective cross-sectional study was performed on schistosomiasis patients at a University Hospital in Northeastern Brazil. We evaluated 41 serum samples from 10 patients with mild PPF (C Niamey pattern) and 31 patients with significant PPF (D/E/F Niamey patterns). MM were built using partial least squares-discriminant analysis (PLS-DA) and orthogonal projections to latent structures discriminant analysis (OPLS-DA) formalisms. RESULTS PLS-DA and OPLS-DA resulted in discrimination between mild and significant PPF groups with R2 and Q2 values of 0.80 and 0.38 and 0.72 and 0.42 for each model, respectively. The OPLS-DA model presented accuracy, sensitivity, and specificity values of 92.7%, 90.3%, and 100% to discriminate significant PPF. The metabolites identified as responsible by discrimination were: N-acetylglucosamines, alanine, glycolaldehyde, carbohydrates, and valine. CONCLUSION MMs discriminated mild from significant PPF patterns in patients with Schistosomiasis mansoni through identification of differences in serum metabolites profiles.

Evaluation of the -C592a Promoter Polymorphism and Putative Haplotypes of the Interleucin-10 (Il-10) Gene in Schistosomic High Digestive Hemorrhage in Pernambuco

“…Schistosomiasis Mansoni (MS) is a neglected disease that affects around 240 million people worldwide (Rodrigues et al, 2022). In Brazil, the parasitosis is caused by Schistosoma mansoni and is considered endemic in the state of Pernambuco, with cases reported in 102 of its 185 municipalities (Rodrigues et al, 2022).…”

“…Schistosomiasis Mansoni (MS) is a neglected disease that affects around 240 million people worldwide (Rodrigues et al, 2022). In Brazil, the parasitosis is caused by Schistosoma mansoni and is considered endemic in the state of Pernambuco, with cases reported in 102 of its 185 municipalities (Rodrigues et al, 2022). It is a public health problem associated with the diseaseimpoverishment-poverty cycle, and its epidemiological profile is determined by the interconnection of ecological, biological, socioeconomic and cultural factors of individuals (Melo et al, 2019).…”