Assessment of partial nitrification reactor performance through microbial population shift using quinone profile, FISH and SEM

Sinha, Banashri; Annachhatre, Ajit P.

doi:10.1016/j.biortech.2006.11.034

Cited by 57 publications

(15 citation statements)

References 20 publications

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“…The samples were pretreated by overnight fixing with 2.5% gluteraldehyde in a 0.1-M phosphate buffer. The samples were then washed and dehydrated sequentially in 30, 50, 70, 90, and 100% ethanol solutions, respectively (Sinha and Annachhatre, 2007).…”

Section: Scanning Electron Microscopy (Sem) Observationmentioning

confidence: 99%

Stable Partial Nitrification in an Up-Flow Fixed-Bed Bioreactor Under an Oxygen-Limiting Environment

Bagchi

Biswas

Roychoudhury³

et al. 2009

Environmental Engineering Science

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Stable partial nitrification followed by a denitrification or anammox process is a cost-effective alternative to conventional biological nitrogen removal processes, especially for wastewaters having a low carbon-to nitrogen (C=N) ratio. This article addresses partial nitrification in an up-flow fixed-film bioreactor operated for more than 265 days using low C=N synthetic wastewater. The study was carried out in the absence of external aeration, for further cutting down the cost of treatment. By controlling influent dissolved oxygen (DO) at 1 mg=L, stable partial nitrification with more than 85% efficiency was achieved (influent NH 3 N: 102.6 AE 4.6 mg N=L). Maximum ammonium removal rate of 210.5 AE 6.7 mg N=L=day was obtained at a hydraulic retention time (HRT) of 18 h. Oxygen uptake at influent DO concentration of 1 mg=L was practically negligible compared to the amount of ammonia oxidized to nitrite. Partial nitrification was taking place devoid of oxygen consumption. A possible mechanism of partial nitrification with negligible DO uptake is proposed in this article. The nitrogen oxide (NOx) cycle may be playing a major role for such low oxygen requirement. This strategy of developing a stable partial nitrifying biosystem under a low oxygen environment without external aeration, can lead to considerable savings in aeration cost in the treatment of ammonia laden wastewaters.

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Section: Scanning Electron Microscopy (Sem) Observationmentioning

confidence: 99%

Stable Partial Nitrification in an Up-Flow Fixed-Bed Bioreactor Under an Oxygen-Limiting Environment

Bagchi

Biswas

Roychoudhury³

et al. 2009

Environmental Engineering Science

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“…The bacterial morphologies of the sludge samples were observed using scanning electron microscopy (Sinha and Annachhatre, 2007). The samples were pretreated by washing with deionized and fixing with 2.5% glutaraldehyde solution (pH = 6.8, 4°C).…”

Section: Sem Analysismentioning

confidence: 99%

Effect of trace hydrazine addition on the functional bacterial community of a sequencing batch reactor performing completely autotrophic nitrogen removal over nitrite

Xiao

Zhang

et al. 2015

Bioresource Technology

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“…Quantification of changes in microbial population of a mixed culture of nitrifiers, denitrifiers, NOBs, AOBs and ANAMMOX using quinone profiles, since this profile is usually represented as the mole fraction of each quinone type that is specific for a microbial community [70].…”

Section: Molecular Signaturesmentioning

confidence: 99%

State of The Art Strategies for Successful ANAMMOX Startup and Development: A Review

Suneethi¹,

S²,

Joseph³

2014

Int J Waste Resources

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ANAMMOX (Anaerobic ammonium oxidation) bacteria, leading proponent of autotrophic ammonia removal, requires dedicated enrichment and cultivation techniques due to its slow growth rate and low biomass yield. Sensitivity to inhibitory concentrations of NO 2 --N, O 2 etc., often present in industrial effluents makes ANAMMOX startup difficult to achieve. In this paper, significant parameters for ANAMMOX startup and development such as source of seed, type of reactors used, one and two stage ANAMMOX process, operational strategies and experimental conditions promoting long term enrichment, cultivation and quantitative analysis with biomass retention are discoursed in detail. Key chemical and molecular signatures including NO 2 --N, Alkalinity, O 2 , Polymerase chain reaction (PCR), Fluorescence in situ hybridization (FISH), Scanning electron microscopy (SEM), inhibitors and affinity factors for ANAMMOX activity are deliberated. Summary of state of the art on ANAMMOX enrichment, recommendations for future research with specific contributions of this paper to scientific community is brought out in conclusion. State of The

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Assessment of partial nitrification reactor performance through microbial population shift using quinone profile, FISH and SEM

Cited by 57 publications

References 20 publications

Stable Partial Nitrification in an Up-Flow Fixed-Bed Bioreactor Under an Oxygen-Limiting Environment

Stable Partial Nitrification in an Up-Flow Fixed-Bed Bioreactor Under an Oxygen-Limiting Environment

Effect of trace hydrazine addition on the functional bacterial community of a sequencing batch reactor performing completely autotrophic nitrogen removal over nitrite

State of The Art Strategies for Successful ANAMMOX Startup and Development: A Review

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