Effect of trace hydrazine addition on the functional bacterial community of a sequencing batch reactor performing completely autotrophic nitrogen removal over nitrite

Xiao, Peng; Lu, Peili; Zhang, Daijun; Han, Xiaohu; Yang, Qi

doi:10.1016/j.biortech.2014.10.084

Cited by 39 publications

(5 citation statements)

References 32 publications

(34 reference statements)

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“…13,14 In the process of nitrite autotrophic denitrification, long-term addition of hydrazine can restore and improve the ability of autotrophic denitrification (Canon). 15,16 This phenomenon was also observed in a moving-bed biofilm reactor and batch experiments with anammox enrichment culture. 17 Furthermore, research demonstrated that extra hydrazine increased the specific anammox activity (SAA) by 42%, of which 5% was due to the increased ammonia removal rate by enhancing anammox; a biological reaction (the synthesis of azide by combining exogenous hydrazine and nitrite) was responsible for 25%; and 12% of the reduction in nitrate production rate was by selective inhibition of nitrite oxidoreductase (nxr) activity.…”

Section: Introductionmentioning

confidence: 55%

“…13,26 N 2 H 4 was easily oxidized by anammox bacteria, which provided additional metabolized energy, helping to cut down the start-up time of the anammox process. 15 The key to maintain cell viability depends on the anammox metabolic reaction pathway for degrading substrates. Thus, the pathway of hydrazine oxidation released four electrons, which would be transported into the electron transport chain to produce energy in the form of adenosine triphosphate (ATP).…”

Section: Recovery Of Anammox After Inhibitionmentioning

confidence: 99%

See 1 more Smart Citation

Recovery of anaerobic ammonium oxidation via hydrazine following sulfate inhibition

Chen¹,

zhou²,

Wang³

et al. 2022

Environ. Sci.: Water Res. Technol.

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Section: Introductionmentioning

confidence: 55%

Section: Recovery Of Anammox After Inhibitionmentioning

confidence: 99%

Recovery of anaerobic ammonium oxidation via hydrazine following sulfate inhibition

Chen¹,

zhou²,

Wang³

et al. 2022

Environ. Sci.: Water Res. Technol.

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“…Hydrazine is the intermedium of anammox process. Long-term addition of 4 mg/L hydrazine had strong inhibition effect on NOB, meanwhile, the effect of promoting anammox bacteria growth and partially inhibiting AOB also improved the nitrogen removal efficiency of PN/A process27. Hydroxylamine is also an intermedium of AOB and anammox bacteria, so it can promote both of them.…”

mentioning

confidence: 94%

In-situ restoration of one-stage partial nitritation-anammox process deteriorated by nitrate build-up via elevated substrate levels

Wang

Gao

2016

Sci Rep

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The one-stage partial nitritation and anammox process (PN/A) has been a promising microbial process to remove ammonia from wastewater especially with low carbon/nitrogen ratio. The main breakdown was the deterioration caused by overgrowth of nitrite oxidizing bacteria (NOB) resulting effluent nitrate build-up in the PN/A process. This study presented an in-situ restoring strategy for suppressing NOB activity in a one-stage granular PN/A system deteriorated over 2 months, using elevated concentrations of substrates (ammonia and nitrite) under limited dissolved oxygen level. The results showed that the NOB activity was successfully suppressed after 56 days of restoration, and finally the ratio of produced nitrate/consumed ammonium was reduced from 36.8% to 7%. On day 66 the nitrogen removal rate obtained as 1.2 kg N/(m3·d). The high FA level (5–40 mg/L) and low dissolved oxygen (<0.13 mg/L) were responsible for NOB suppression. From quantitative PCR (qPCR) analysis, after this restoration, anammox bacteria had a widely growth, and AOB stay stable, but Nitrospira increase and Nitrobacter declined. High amount of NOB was still persistent in the granules, which was not easy to wash-out and threaten the deammonification performance.

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“…showed the lowest nitrogen removal efficiency. Combined with the data obtained in phase I, the consumption of the substrate by anammox bacteria is greatly reduced in environments with a high concentration of NO2 --N (concentration > 100 mg/L); therefore, its activity was inhibited by high concentrations of NO2 --N. After the addition of N2H4, the consumption of NH4 + -N and NO2 --N in B5, B10, B15, and B20 were significantly greater than that of B0, indicating that N2H4 had a restorative effect on anammox bacteria activity (Xiao et al, 2015;Zekker et al, 2012). As shown in Figure 5 1-2 μm can be observed.…”

Section: Effect Of N2h4 On Anammox Inhibited By High Concentrations Of No2 --Nmentioning

confidence: 99%

Recovery of nitrogen removal by N2H4 after nitrite inhibited anammox reaction

2021

Global NEST: The International Journal

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<p>A pilot-scale sequencing batch reactor (SBR) for anaerobic ammonium oxidation (anammox) of bacteria culture was used along with a batch experimental reaction device to study the effect of NO2--N concentration on the activity of anammox bacteria and the recovery of N2H4 on anammox bacteria after inhibition by high concentrations of NO2--N. The optimal influent NO2--N concentration in the pilot-scale reactor was 72.0 mg/L, with its total nitrogen consumption being approximately 40.0 g/d. Influent water NO2--N concentrations greater than 100 mg/L had a serious inhibitory effect on the anammox bacteria. At an influent NO2--N concentration of 120.35 mg/L, the addition of 10.0-15.0 mg/L of N2H4, restored the activity of granular anammox bacteria; the total nitrogen consumption was increased by 69.96%. Microbiological analysis showed that a change in NO2--N concentration within the range of 18.87-115.39 mg/L did not affect the microbial population structure of the pilot-scale reactor, wherein Candidatus Kuenenia was the dominant bacterial species. In samples collected at stages A0 (sludge inoculation), A20 (the number indicates the NO2--N concentration, which, in this stage, was 20 mg/L), A40, A60, A80, and A100, the proportion of Candidatus Kuenenia was 27%, 23%, 36%, 26%, 34%, and 33%, respectively.</p>

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Effect of trace hydrazine addition on the functional bacterial community of a sequencing batch reactor performing completely autotrophic nitrogen removal over nitrite

Cited by 39 publications

References 32 publications

Recovery of anaerobic ammonium oxidation via hydrazine following sulfate inhibition

Recovery of anaerobic ammonium oxidation via hydrazine following sulfate inhibition

In-situ restoration of one-stage partial nitritation-anammox process deteriorated by nitrate build-up via elevated substrate levels

Recovery of nitrogen removal by N2H4 after nitrite inhibited anammox reaction

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