Assessment of Occurrence of Hepatitis B Virus Genotypes among Population in Provisional Capital and Federal Capital of Pakistan

Iqbal, Javed; Raza, Abida; Din, Ismail; Khattak, Jabar Zaman Khan

doi:10.9734/bmrj/2015/19015

Cited by 2 publications

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“…The results obtained by genotype specific primers in separate mixture correlate well with those obtained by sequence analysis of HBV samples. Among all the samples, genotype D was the pre dominant one and was identified with a very high viral load [33]. Mixed genotype A+D was also present with high viral load but its proportion was very low as compared to genotype D.…”

Section: Discussionmentioning

confidence: 97%

HBV Genotyping and Phylogenetic Analysis through Multiplex PCR Using Type Specific Primer Pairs in Pakistani Population

Iqbal¹,

Raza²

2016

J Bacteriol Parasitol

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Background: Hepatitis B virus causing acute and chronic hepatocellular carcinoma relics a serious health issue affecting more than 350 million people worldwide. Geographically, nine genotypes of HBV have been identified so far. Four open reading frames named (ORF P, S, F and X) renders it to specifically targets hepatocyte cells. Additionally, reverse transcription process present in the life cycle of HBV caused four order higher mutation rates as compared to adenovirus. The structural and functional differences between HBV genotypes are the mainstay to severity, complications, treatment and possibly vaccination against the virus. Hepatitis B genotyping is important for assessing its clinical implications and geographical distribution, but the sub-genotypes have been found useful for determination of specific genomic markers related to hepatocarcinogenesis and medical therapies. In Pakistan, there is no reported data available on molecular evolutionary analysis of HBV. A study was, therefore, much needed to evaluate the spectra of dominant strains prevalent here.Objectives: This study was aimed to figure out the prevalence of HBV genotypes among population in Pakistan. In this research, we focused to find out the new strains and sub genotypes of HBV. Pakistan is one of the HBV endemic countries but there is no data on subgenotypes and their recombination or the phylogenetic relatedness of the virus endemic in the country. The present study was carried out in this perspective. This study will help future researcher to study some other aspects of HBV related strains in Pakistan and also about its medical therapies. , for further analysis. All the samples were genotyped by type specific nested PCR primer pair method for 8 HBV genotypes from A through H. The patients were randomly selected irrespective of their age and gender and a written consent (parental consent in case of less than 18 years of age) was obtained. The study was approved by ethical review committee. In our research, we have used the type specific primer technique to find out the dominant strain in Pakistani populations and furthermore its phylogenetic analysis with reference sequences retrieved from NCBI databases. Results:Our results showed a direct relationship between viral load and identified genotypes and pinpointed that genotype D is the most pre-dominant genotype and prevailing in high proportions among all genotypes in Pakistani population. Mix HBV genotype infections of genotype A with B & C, D with B and C constitute about 17 percent of all the samples. Subgenotypes of different HBV strains have been identified on the basis of differences in the complete nucleotide sequences. Positive PCR results were repeated twice for confirmation. Furthermore, phylogenetic analysis revealed a homology between our reported sequences and reference sequences. The phylogenetic method showed that genotypes/sub-genotypes varies in the geographical areas and correlate strongly with ethnicity. Conclusion:Genotype D was identified and come out to be the...

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Section: Discussionmentioning

confidence: 97%

HBV Genotyping and Phylogenetic Analysis through Multiplex PCR Using Type Specific Primer Pairs in Pakistani Population

Iqbal¹,

Raza²

2016

J Bacteriol Parasitol

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“…In addition, only a single study is conducted showing the spread of disease in drug users, with a prevalence of 22.40% . However, about 4 to 4.8 million drug users are present in Pakistani population (Hussain, 2008).…”

Section: Prevalence Of Hepatitis B In Different Population Groups Gen...mentioning

confidence: 99%

Prevalence of Hepatitis B Virus and Genotypes in the Region of Khyber Pakhtunkhwa Pakistan

GOHAR,

REHMAN,

AHMAD

et al. 2023

Bull. Biol. All. Sci. Res.

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About 3% of Pakistani population is the carrier of hepatitis B virus, and infection is growing at a steady rate. The current study reviews the situation of HBV in the KP population. Approximately 85 studies related to the prevalence of HBV and its genotypes in this region were searched using various databases. The mean and standard deviation based on collected data indicates an HBV prevalence of 110.09%±3.71 in general population, 2.51% ± 0.01 in healthy blood donors, 1.74% ± 0.01 in health care workers, 3.40% ±0.00 in healthy children, 1.27% ± 0.00 in pregnant women, 22.40% in drug abusers. The percent prevalence of infection is 5.98% ± 0.02 in multi-transfused people, 26.61% ± 0.12 in patients with liver disease, and 4.11% ± 0.02 in patients with dental treatment and ophthalmic, respectively. Genotype D is the most prevalent genotype, with a value of 41.77%. The area must be vaccinated, and more preventive care and immunization services should be provided.

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Assessment of Occurrence of Hepatitis B Virus Genotypes among Population in Provisional Capital and Federal Capital of Pakistan

Cited by 2 publications

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HBV Genotyping and Phylogenetic Analysis through Multiplex PCR Using Type Specific Primer Pairs in Pakistani Population

HBV Genotyping and Phylogenetic Analysis through Multiplex PCR Using Type Specific Primer Pairs in Pakistani Population

Prevalence of Hepatitis B Virus and Genotypes in the Region of Khyber Pakhtunkhwa Pakistan

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